FastQCFastQC Report
Sun 1 Jan 2017
ERR1142854_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142854_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207757
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15110.7272919805349519No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3350.16124607113117728No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2770.13332884090548092No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2110.10156095823486092No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC150.004230400559.51506876-77
TATTGTC600.001304409639.6767125
GATTCAA350.002790071533.9922252-53
ATGTAAG1152.7483908E-531.0513423
GTAAGTA1055.8804813E-428.340512
ATTTAGG850.007171155428.0206051
CTATTGA850.00722199427.9801039
TATGTAA1107.7182794E-427.0523052
GCTAGCT557.7532046E-427.03926528-29
GGTATCA16600.026.9023151
ACGCATC450.00949914926.45752396-97
TTGGCTC900.00949956926.4511434
TAGGGAC900.00949956926.4511434
GACACTC1859.255E-725.7114478
ACGGATT600.001287288724.78897938-39
GTATCAA20800.024.6190661
CTATAGA1250.001622067223.8175141
TGTAAGT1350.002537424222.0532551
GAGTCAT700.003158698321.24257756-57
TTATTGT1754.7571165E-420.4051694
Produced by FastQC (version 0.11.5)