##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142854_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207757
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07168470857781	33.0	33.0	33.0	27.0	33.0
2	31.139167392675095	33.0	33.0	33.0	27.0	33.0
3	31.213369465288775	33.0	33.0	33.0	27.0	33.0
4	31.12674903854022	33.0	33.0	33.0	27.0	33.0
5	31.35299893625726	33.0	33.0	33.0	27.0	33.0
6	34.6622207675313	37.0	37.0	37.0	27.0	37.0
7	34.69006579802365	37.0	37.0	37.0	27.0	37.0
8	34.584124722632694	37.0	37.0	37.0	27.0	37.0
9	34.3707023108728	37.0	37.0	37.0	27.0	37.0
10-11	34.52393661826076	37.0	37.0	37.0	27.0	37.0
12-13	34.474080295730104	37.0	37.0	37.0	27.0	37.0
14-15	34.459327483550496	37.0	37.0	37.0	27.0	37.0
16-17	34.51317645133497	37.0	37.0	37.0	27.0	37.0
18-19	34.48563225306488	37.0	37.0	37.0	27.0	37.0
20-21	34.4497321389893	37.0	37.0	37.0	27.0	37.0
22-23	34.537654567595794	37.0	37.0	37.0	27.0	37.0
24-25	34.659108959024245	37.0	37.0	37.0	27.0	37.0
26-27	34.727460446579414	37.0	37.0	37.0	27.0	37.0
28-29	34.84115817998912	37.0	37.0	37.0	27.0	37.0
30-31	34.866690412356746	37.0	37.0	37.0	27.0	37.0
32-33	34.914840414522736	37.0	37.0	37.0	27.0	37.0
34-35	34.95161173871398	37.0	37.0	37.0	27.0	37.0
36-37	34.94832665084691	37.0	37.0	37.0	27.0	37.0
38-39	34.93306122056056	37.0	37.0	37.0	27.0	37.0
40-41	34.91650341504739	37.0	37.0	37.0	27.0	37.0
42-43	34.90881895676199	37.0	37.0	37.0	27.0	37.0
44-45	34.912888615064716	37.0	37.0	37.0	27.0	37.0
46-47	34.91588490399842	37.0	37.0	37.0	27.0	37.0
48-49	34.89409502447571	37.0	37.0	37.0	27.0	37.0
50-51	34.894513782929096	37.0	37.0	37.0	27.0	37.0
52-53	34.86497927867653	37.0	37.0	37.0	27.0	37.0
54-55	34.876646755584645	37.0	37.0	37.0	27.0	37.0
56-57	34.83249661864582	37.0	37.0	37.0	27.0	37.0
58-59	34.78723941912908	37.0	37.0	37.0	27.0	37.0
60-61	34.77033264823808	37.0	37.0	37.0	27.0	37.0
62-63	34.718572659404984	37.0	37.0	37.0	27.0	37.0
64-65	34.671000255105724	37.0	37.0	37.0	27.0	37.0
66-67	34.628327806042634	37.0	37.0	37.0	27.0	37.0
68-69	34.56220007027441	37.0	37.0	37.0	27.0	37.0
70-71	34.48597399846936	37.0	37.0	37.0	27.0	37.0
72-73	34.43503949325414	37.0	37.0	37.0	27.0	37.0
74-75	34.3873780426171	37.0	37.0	37.0	27.0	37.0
76-77	34.30583085046473	37.0	37.0	37.0	27.0	37.0
78-79	34.271762202958264	37.0	37.0	37.0	27.0	37.0
80-81	34.212426055439764	37.0	37.0	37.0	27.0	37.0
82-83	34.152141684756714	37.0	37.0	37.0	27.0	37.0
84-85	34.08695735883749	37.0	37.0	37.0	27.0	37.0
86-87	34.03941383443157	37.0	37.0	37.0	24.5	37.0
88-89	33.9708650009386	37.0	37.0	37.0	22.0	37.0
90-91	33.89741380555168	37.0	37.0	37.0	22.0	37.0
92-93	33.863205571894085	37.0	37.0	37.0	22.0	37.0
94-95	33.80905336522957	37.0	37.0	37.0	22.0	37.0
96-97	33.7060002791723	37.0	37.0	37.0	22.0	37.0
98-99	33.60810466073345	37.0	33.0	37.0	22.0	37.0
100-101	33.53166680304394	37.0	33.0	37.0	22.0	37.0
102-103	33.45241796906963	37.0	33.0	37.0	22.0	37.0
104-105	33.33193346072575	37.0	33.0	37.0	22.0	37.0
106-107	33.263211347872755	37.0	33.0	37.0	14.0	37.0
108-109	33.157592764624056	37.0	33.0	37.0	14.0	37.0
110-111	33.0266874281011	37.0	33.0	37.0	14.0	37.0
112-113	32.92561261473741	37.0	33.0	37.0	14.0	37.0
114-115	32.81086798519424	37.0	33.0	37.0	14.0	37.0
116-117	32.705523760932245	37.0	33.0	37.0	14.0	37.0
118-119	32.57523452880048	37.0	33.0	37.0	14.0	37.0
120-121	32.48938663919868	37.0	33.0	37.0	14.0	37.0
122-123	32.29852183079271	37.0	33.0	37.0	14.0	37.0
124-125	30.43287350125387	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	153.0
1	9.0
2	18.0
3	5.0
4	8.0
5	9.0
6	8.0
7	12.0
8	8.0
9	9.0
10	4.0
11	6.0
12	7.0
13	13.0
14	24.0
15	77.0
16	119.0
17	270.0
18	572.0
19	1024.0
20	1677.0
21	2207.0
22	2363.0
23	2544.0
24	2523.0
25	2483.0
26	2829.0
27	3145.0
28	3592.0
29	4323.0
30	5190.0
31	5920.0
32	7477.0
33	9804.0
34	15247.0
35	28709.0
36	105369.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46487572447884	21.58718075957661	12.580276831612572	22.367666684331986
2	16.745636571340434	25.5173211608817	35.757701373256914	21.979340894520952
3	19.04054699140263	31.115440066734497	27.89636865986142	21.947644282001455
4	13.691819532048685	22.857473525837623	33.157321142680786	30.293385799432905
5	14.109458879365647	41.17079617419657	29.448847001625435	15.27089794481235
6	26.97234618832352	38.71244469719606	18.26751616914224	16.04769294533818
7	24.482037543846126	37.672975369078365	20.3956751339475	17.44931195312801
8	25.168140910755238	36.254841880094816	19.680676803299225	18.896340405850726
9	26.82611879890529	21.398354083953283	18.910688817792852	32.86483829934857
10-11	24.178860691795112	30.909384380405402	23.89747864339162	21.014276284407867
12-13	25.264651604292247	29.872504661819338	24.096426179428246	20.76641755446017
14-15	24.429239146147545	29.175059027610466	22.80585939382258	23.58984243241941
16-17	23.080333157454724	31.27362615917255	23.33114087394804	22.314899809424684
18-19	23.291760839288898	30.055081948252848	25.408054590403307	21.245102622054947
20-21	26.132598948055737	27.747138234535868	24.758276893126222	21.36198592428218
22-23	26.21512677121909	27.55403709049029	24.127130221497257	22.103705916793363
24-25	24.47665483067465	28.27364749300199	24.36897460481116	22.880723071512197
26-27	23.742449395590228	28.113427125071983	24.841157209185777	23.302966270152012
28-29	23.72010650217462	28.07681650060841	25.181740419020997	23.02133657819597
30-31	24.543818264617933	27.683900332754245	25.068370034287422	22.703911368340396
32-33	23.781676413255358	27.980540556169984	25.0558410860278	23.18194194454685
34-35	23.558936497303655	28.310193105641844	25.29818845992588	22.832681937128623
36-37	23.746030522212212	28.520005204292577	24.79363534292915	22.94032893056606
38-39	23.810361361018884	27.95885717590027	24.880614492027313	23.350166971053532
40-41	23.208041431397923	28.232249108930933	25.053801533193077	23.505907926478066
42-43	23.682885036667862	28.21509564010305	24.80497227316655	23.297047050062538
44-45	23.668399476541996	28.21804966102961	25.042597238590325	23.070953623838065
46-47	23.59670516107887	28.18494669262951	25.027955040583006	23.190393105708612
48-49	23.092494324836252	28.663071191374716	25.268094253505108	22.976340230283927
50-51	23.19544024967766	28.533384747008565	25.127309096605494	23.14386590670828
52-53	22.97626594101073	28.394823675730134	24.81820438719754	23.8107059960616
54-55	22.65495469442838	28.082465779834198	25.09784075573549	24.16473877000193
56-57	23.23408070019857	27.932274295849318	24.605511750303638	24.228133253648473
58-59	23.197211851559054	28.192403452406488	25.05260316366152	23.55778153237294
60-61	23.65902321950091	27.946775390606177	25.42458240561355	22.969618984279368
62-63	23.040512857590436	27.74492083001952	25.532740462246643	23.681825850143397
64-65	22.93321958660777	27.620934217280347	25.452757755622873	23.993088440489014
66-67	23.38868846651581	27.674433736507638	25.12043416546775	23.816443631508807
68-69	23.313275223454735	27.803094748663128	24.917281383069735	23.966348644812406
70-71	23.385302815221138	28.05267483364383	24.87304873434348	23.68897361679155
72-73	23.681109335801096	28.233213078170678	24.81039930015833	23.2752782858699
74-75	23.87084800586178	27.652082113133332	25.22373346252389	23.253336418481
76-77	23.518465398920593	27.318570013281686	25.370428846566405	23.792535741231315
78-79	23.51489324778373	27.623009694229257	25.373717117473234	23.488379940513777
80-81	23.695203110349432	27.781754898487005	25.2973801621219	23.22566182904166
82-83	23.65685222903836	27.46869463560435	25.538428163378445	23.336024971978837
84-85	23.489423250768397	28.055204001687457	24.766106189356957	23.68926655818719
86-87	23.082059205657828	28.167642500222968	25.109736079005156	23.64056221511405
88-89	23.280763788960154	27.259996288074355	25.46260475945999	23.996635163505502
90-91	23.82784346337877	27.24277157078979	25.196032348651936	23.733352617179495
92-93	23.510027962587987	27.841577475653263	25.104859704946485	23.543534856812265
94-95	22.795599394482853	28.032165688004397	25.196455603444118	23.97577931406863
96-97	22.76367300497551	28.064373047402324	25.129932117098008	24.042021830524163
98-99	22.431914354949544	28.457207614500824	24.910605567410514	24.200272463139115
100-101	22.771897770632318	28.784610155927986	24.818075980244462	23.625416093195234
102-103	22.845544525814894	28.616471972191555	25.111248246303376	23.426735255690172
104-105	23.135114546142702	28.561682308328372	25.11023092444511	23.192972221083817
106-107	22.895963818533357	28.47144875336911	25.114393029860317	23.518194398237213
108-109	22.549539559326938	28.88939781109879	25.087507834723493	23.473554794850777
110-111	22.229293221482983	29.634557901841674	24.866695593481825	23.26945328319352
112-113	22.336791299714793	29.854312171672	24.440742446195795	23.368154082417412
114-115	22.428038604434736	30.022590779008173	24.395148166096472	23.154222450460615
116-117	22.322080764882152	29.748149148162167	24.585773211285193	23.343996875670488
118-119	22.205267281383716	29.3244139094799	25.030072922316638	23.440245886819746
120-121	22.506989973004245	29.224354030080985	25.13208638642499	23.13656961048978
122-123	22.376612765218983	29.832993084964077	24.977765351548474	22.81262879826847
124-125	22.204755165172188	29.80597903792414	24.607172048636645	23.382093748267025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	980.0
1	687.0
2	280.0
3	144.0
4	116.0
5	101.0
6	89.0
7	95.0
8	98.0
9	94.5
10	97.0
11	88.5
12	95.0
13	116.5
14	124.5
15	140.0
16	150.5
17	173.0
18	228.0
19	275.0
20	326.0
21	371.5
22	431.5
23	521.5
24	604.0
25	723.0
26	852.0
27	974.5
28	1137.5
29	1499.5
30	1946.0
31	2258.0
32	2766.5
33	3358.0
34	3776.0
35	4181.5
36	4604.0
37	4960.5
38	5067.5
39	5079.0
40	4907.0
41	4703.0
42	4795.5
43	5238.0
44	5794.5
45	6136.5
46	6625.0
47	7287.0
48	7906.5
49	8505.5
50	9162.5
51	9031.5
52	8336.5
53	8099.0
54	7964.5
55	7751.5
56	7521.5
57	6919.0
58	5909.5
59	4955.0
60	4206.5
61	3454.0
62	2718.5
63	2100.0
64	1604.5
65	1291.0
66	1062.0
67	825.5
68	661.5
69	556.5
70	461.0
71	389.0
72	334.0
73	309.0
74	234.0
75	182.0
76	156.5
77	134.5
78	103.0
79	68.5
80	56.0
81	36.0
82	36.5
83	41.0
84	27.0
85	17.0
86	11.0
87	6.0
88	3.5
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09337832178939819
2	0.14151147735094366
3	0.1771300124664873
4	0.18531264891195
5	0.20600990580341458
6	0.12659019912686456
7	0.10252362134609183
8	0.09289699023378274
9	0.10252362134609183
10-11	0.10107962667924547
12-13	0.10589294223540002
14-15	0.10926226312470819
16-17	0.10950292890251592
18-19	0.11937022579263275
20-21	0.11383491290305502
22-23	0.11431624445867046
24-25	0.09530364801186002
26-27	0.11576023912551682
28-29	0.12009222312605591
30-31	0.11864822845920955
32-33	0.11961089157044047
34-35	0.12225821512632545
36-37	0.11359424712524728
38-39	0.11359424712524728
40-41	0.13549483290575046
42-43	0.13790149068382773
44-45	0.13982681690628956
46-47	0.13525416712794272
48-49	0.13188484623863456
50-51	0.13934548535067412
52-53	0.15041611112982956
54-55	0.13332884090548092
56-57	0.1314035146830191
58-59	0.14800945335175228
60-61	0.12466487290440273
62-63	0.14054881423971274
64-65	0.1340508382389041
66-67	0.1340508382389041
68-69	0.13308817512767318
70-71	0.14223347468436684
72-73	0.13549483290575046
74-75	0.1499347795742141
76-77	0.15811741601967683
78-79	0.15113810846325273
80-81	0.15426676357475322
82-83	0.15619208979721502
84-85	0.1658187209095241
86-87	0.15763608446406138
88-89	0.15282276890790683
90-91	0.15859874757529227
92-93	0.1622087342424082
94-95	0.15932074490871548
96-97	0.16413406046487
98-99	0.18747864091221955
100-101	0.15474809513036866
102-103	0.16269006579802367
104-105	0.1691880417988323
106-107	0.17231669691033275
108-109	0.16702204979856275
110-111	0.1622087342424082
112-113	0.17400135735498684
114-115	0.1788146729111414
116-117	0.16966937335444776
118-119	0.1658187209095241
120-121	0.1525821031300991
122-123	0.14945344801859864
124-125	0.1841093200229114
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	207757.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.41347158968858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.9556348168743	53.90086916162872
2	13.542953583425927	18.259550332735103
3	3.1511143927144283	6.372826817673389
4	1.1230955435948697	3.0284707808255447
5	0.5851367126562247	1.972304857736709
6	0.38728678121474713	1.5665007853489379
7	0.2522743093046557	1.1904680888182404
8	0.17464090242107508	0.9418519611008578
9	0.1373337349420378	0.8332329454938799
>10	0.6316777324815871	7.2550569274088845
>50	0.04217690143193477	1.8970704355551398
>100	0.01569373076537108	1.782677551152547
>500	0.0	0.0
>1k	9.808581728356924E-4	0.9991193545220692
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1511	0.7272919805349519	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	335	0.16124607113117728	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	277	0.13332884090548092	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	211	0.10156095823486092	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	4.8133155561545457E-4	0.0
22-23	0.0	0.0	0.0	0.0019253262224618183	0.0
24-25	0.0	0.0	0.0	0.007219973334231819	0.0
26-27	0.0	0.0	0.0	0.017568601779964093	0.0
28-29	0.0	0.0	0.0	0.035137203559928186	0.0
30-31	0.0	0.0	0.0	0.06546109156370183	0.0
32-33	0.0	0.0	0.0	0.11239091823620864	0.0
34-35	0.0	0.0	0.0	0.16774404713198593	0.0
36-37	0.0	0.0	0.0	0.2604003715879609	0.0
38-39	0.0	0.0	0.0	0.3761606107134778	0.0
40-41	0.0	0.0	0.0	0.5027508098403423	0.0
42-43	0.0	0.0	0.0	0.6074404231867037	0.0
44-45	0.0	0.0	0.0	0.7227193307566051	0.0
46-47	0.0	0.0	0.0	0.8644714738853565	0.0
48-49	0.0	0.0	0.0	1.0141655876817628	0.0
50-51	0.0	0.0	0.0	1.146291099698205	0.0
52-53	0.0	0.0	0.0	1.2776946143812242	0.0
54-55	0.0	0.0	0.0	1.4042848135080888	0.0
56-57	0.0	0.0	0.0	1.5429083015253398	0.0
58-59	0.0	0.0	0.0	1.707764359323633	0.0
60-61	0.0	0.0	0.0	1.8627531202318093	0.0
62-63	0.0	0.0	0.0	2.003301934471522	0.0
64-65	0.0	0.0	0.0	2.153477379823544	0.0
66-67	0.0	0.0	0.0	2.3161674456215673	0.0
68-69	0.0	0.0	0.0	2.502442757644748	0.0
70-71	0.0	0.0	0.0	2.6937720510018917	0.0
72-73	0.0	0.0	0.0	2.8923213176932667	0.0
74-75	0.0	0.0	0.0	3.0706546590487926	0.0
76-77	0.0	0.0	0.0	3.2607806235168972	0.0
78-79	0.0	0.0	0.0	3.4696785186540042	0.0
80-81	0.0	0.0	0.0	3.6877217133478055	0.0
82-83	0.0	0.0	0.0	3.9192421915988387	0.0
84-85	0.0	0.0	0.0	4.134878728514563	2.4066577780772728E-4
86-87	0.0	0.0	0.0	4.361585891209442	7.219973334231819E-4
88-89	0.0	0.0	0.0	4.600567008572515	9.626631112309091E-4
90-91	0.0	0.0	0.0	4.86048604860486	9.626631112309091E-4
92-93	0.0	0.0	0.0	5.128587725082669	9.626631112309091E-4
94-95	0.0	0.0	0.0	5.378639468224898	9.626631112309091E-4
96-97	0.0	0.0	0.0	5.64096516603532	9.626631112309091E-4
98-99	0.0	0.0	0.0	5.950461356296057	9.626631112309091E-4
100-101	0.0	0.0	0.0	6.278729477225798	9.626631112309091E-4
102-103	0.0	0.0	0.0	6.592557651487073	0.0014439946668463638
104-105	0.0	0.0	0.0	6.933821724418431	0.0014439946668463638
106-107	0.0	0.0	0.0	7.287119086240175	0.0014439946668463638
108-109	0.0	0.0	0.0	7.625254504060032	0.0014439946668463638
110-111	0.0	0.0	0.0	7.9917884836612005	0.0014439946668463638
112-113	0.0	0.0	0.0	8.39755098504503	0.0014439946668463638
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTC	15	0.0042304005	59.515068	76-77
TATTGTC	60	0.0013044096	39.676712	5
GATTCAA	35	0.0027900715	33.99222	52-53
ATGTAAG	115	2.7483908E-5	31.051342	3
GTAAGTA	105	5.8804813E-4	28.34051	2
ATTTAGG	85	0.0071711554	28.020605	1
CTATTGA	85	0.007221994	27.980103	9
TATGTAA	110	7.7182794E-4	27.052305	2
GCTAGCT	55	7.7532046E-4	27.039265	28-29
GGTATCA	1660	0.0	26.902315	1
ACGCATC	45	0.009499149	26.457523	96-97
TTGGCTC	90	0.009499569	26.451143	4
TAGGGAC	90	0.009499569	26.451143	4
GACACTC	185	9.255E-7	25.711447	8
ACGGATT	60	0.0012872887	24.788979	38-39
GTATCAA	2080	0.0	24.619066	1
CTATAGA	125	0.0016220672	23.817514	1
TGTAAGT	135	0.0025374242	22.053255	1
GAGTCAT	70	0.0031586983	21.242577	56-57
TTATTGT	175	4.7571165E-4	20.405169	4
>>END_MODULE