FastQCFastQC Report
Sun 1 Jan 2017
ERR1142854_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142854_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207757
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.9467791698955992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.7821637778751137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10490.5049168018406118No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.22574449958364823No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.1920512906905664No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3850.18531264891195No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3270.15739541868625365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.13766082490602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.12370220979317183No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2460.11840756268140183No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.11600090490332456No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCTC401.7650511E-459.5240823
GTAAGTA1801.4551915E-1136.3758282
GGTATCA17350.034.3243561
CTTACAC700.00277823734.013763
GTATCAA24050.033.4287831
ATGTAAG1251.2673299E-633.3334853
ATATGTA1301.7151142E-632.0668681
TAAGGTG1004.4220156E-429.7548755
ATAAGAG1055.875307E-428.3447993
TGTAAGT1903.655623E-828.2092021
CTTTACC850.00716590528.0248281
TATCAAC29200.027.5196952
TTATCCT1306.336048E-527.472654
TATGTAA1306.336048E-527.472652
CTAGAAC450.00952566226.44241174-75
CTAAATT450.00952566226.44241172-73
CAACGCA31500.026.2598575
ATCAACG30700.026.1750833
TCAACGC32000.026.0417844
AACGCAG32750.025.9781556
Produced by FastQC (version 0.11.5)