##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142854_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207757
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.778139846070168	33.0	14.0	33.0	14.0	33.0
2	30.166271172571804	33.0	27.0	33.0	27.0	33.0
3	29.721000977103056	33.0	27.0	33.0	27.0	33.0
4	31.137853357528265	33.0	33.0	33.0	27.0	33.0
5	31.9111558214645	33.0	33.0	33.0	27.0	33.0
6	34.6247972390822	37.0	37.0	37.0	27.0	37.0
7	35.79323921696982	37.0	37.0	37.0	33.0	37.0
8	35.65683948073952	37.0	37.0	37.0	33.0	37.0
9	35.99072474092329	37.0	37.0	37.0	33.0	37.0
10-11	36.00744379250759	37.0	37.0	37.0	33.0	37.0
12-13	36.061882391447696	37.0	37.0	37.0	37.0	37.0
14-15	36.13757177856823	37.0	37.0	37.0	37.0	37.0
16-17	36.157881563557424	37.0	37.0	37.0	37.0	37.0
18-19	36.18046323348912	37.0	37.0	37.0	37.0	37.0
20-21	36.118616941908094	37.0	37.0	37.0	37.0	37.0
22-23	36.17023734459008	37.0	37.0	37.0	37.0	37.0
24-25	36.20108107067391	37.0	37.0	37.0	37.0	37.0
26-27	36.13786298415938	37.0	37.0	37.0	37.0	37.0
28-29	36.13124467526967	37.0	37.0	37.0	37.0	37.0
30-31	36.083313678961474	37.0	37.0	37.0	37.0	37.0
32-33	36.003634053244895	37.0	37.0	37.0	37.0	37.0
34-35	35.905375029481554	37.0	37.0	37.0	37.0	37.0
36-37	35.80736148481158	37.0	37.0	37.0	33.0	37.0
38-39	35.73810509393185	37.0	37.0	37.0	33.0	37.0
40-41	35.63847427523501	37.0	37.0	37.0	33.0	37.0
42-43	35.5262807029366	37.0	37.0	37.0	33.0	37.0
44-45	35.39108910891089	37.0	37.0	37.0	33.0	37.0
46-47	35.18470617115187	37.0	37.0	37.0	33.0	37.0
48-49	34.89954851100083	37.0	37.0	37.0	30.0	37.0
50-51	34.81349124217235	37.0	37.0	37.0	27.0	37.0
52-53	34.78496512752879	37.0	37.0	37.0	27.0	37.0
54-55	34.723990046063435	37.0	37.0	37.0	27.0	37.0
56-57	34.68693473625437	37.0	37.0	37.0	27.0	37.0
58-59	34.65317173428573	37.0	37.0	37.0	27.0	37.0
60-61	34.62353374374871	37.0	37.0	37.0	27.0	37.0
62-63	34.61817411687693	37.0	37.0	37.0	27.0	37.0
64-65	34.604444134253	37.0	37.0	37.0	27.0	37.0
66-67	34.580704861930045	37.0	37.0	37.0	27.0	37.0
68-69	34.501862753120236	37.0	37.0	37.0	27.0	37.0
70-71	34.492796873270215	37.0	37.0	37.0	27.0	37.0
72-73	34.490469635198814	37.0	37.0	37.0	27.0	37.0
74-75	34.44420885938861	37.0	37.0	37.0	27.0	37.0
76-77	34.3988939000852	37.0	37.0	37.0	27.0	37.0
78-79	34.322607661835704	37.0	37.0	37.0	27.0	37.0
80-81	34.24912277323989	37.0	37.0	37.0	27.0	37.0
82-83	34.239346929345345	37.0	37.0	37.0	27.0	37.0
84-85	34.197086018762306	37.0	37.0	37.0	27.0	37.0
86-87	34.133239794567686	37.0	37.0	37.0	27.0	37.0
88-89	34.08767935617091	37.0	37.0	37.0	24.5	37.0
90-91	34.01964795410022	37.0	37.0	37.0	22.0	37.0
92-93	33.911925951953485	37.0	37.0	37.0	22.0	37.0
94-95	33.877551658909205	37.0	37.0	37.0	22.0	37.0
96-97	33.8070413993271	37.0	37.0	37.0	22.0	37.0
98-99	33.706019532434524	37.0	37.0	37.0	22.0	37.0
100-101	33.64713583657831	37.0	37.0	37.0	22.0	37.0
102-103	33.55968511289632	37.0	35.0	37.0	18.0	37.0
104-105	33.44411499973527	37.0	33.0	37.0	14.0	37.0
106-107	33.35970147816921	37.0	33.0	37.0	14.0	37.0
108-109	33.28194477201731	37.0	33.0	37.0	14.0	37.0
110-111	33.24339974104362	37.0	33.0	37.0	14.0	37.0
112-113	33.1831442502539	37.0	33.0	37.0	14.0	37.0
114-115	33.03720933590685	37.0	33.0	37.0	14.0	37.0
116-117	32.97387332316119	37.0	33.0	37.0	14.0	37.0
118-119	32.82404684318699	37.0	33.0	37.0	14.0	37.0
120-121	32.71682301920032	37.0	33.0	37.0	14.0	37.0
122-123	32.64787708717395	37.0	33.0	37.0	14.0	37.0
124-125	31.164591325442704	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	5.0
17	7.0
18	33.0
19	169.0
20	699.0
21	1846.0
22	2863.0
23	3717.0
24	3154.0
25	2195.0
26	1858.0
27	1954.0
28	2210.0
29	2744.0
30	3618.0
31	4793.0
32	6520.0
33	10051.0
34	16601.0
35	36037.0
36	106677.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	44.17890179951557	17.317147163487697	13.417634963667796	25.086316073328934
2	16.832488580188976	21.43555087050488	38.75900709015032	22.972953459155825
3	19.735360089336424	27.28659857331267	29.194624411563673	23.78341692578723
4	13.653626767173533	18.601471982748247	35.715557866058234	32.02934338401999
5	14.576173285198557	35.78628158844766	32.17761732851985	17.459927797833934
6	27.700631025669413	35.01446401324625	19.637364805999315	17.64754015508503
7	24.478597592379558	33.791400530427374	23.20451296466545	18.525488912527617
8	25.956766799674618	31.35008688034579	21.71045981603508	20.98268650394451
9	27.80026665768181	16.102465861559416	21.147783227520613	34.949484253238154
10-11	25.006136977334098	25.97866738545512	26.37817257661595	22.637023060594828
12-13	26.73881861414352	25.1323668149175	27.159263751708735	20.96955081923025
14-15	24.94982118532709	24.51156880393922	27.025563518918737	23.513046491814958
16-17	22.316696910332745	26.918467247794297	27.745876191897263	23.01895964997569
18-19	22.296053312411406	25.02304380368364	31.452686197543763	21.228216686361197
20-21	24.3133772309825	23.239280694661044	31.381043146768324	21.066298927588132
22-23	24.167657407451976	23.31281256467893	31.028557401194668	21.49097262667443
24-25	22.840626308620166	24.160437434117743	31.205446747883343	21.793489509378748
26-27	22.34857236373438	24.194006430620536	31.605104064383223	21.852317141261864
28-29	22.078197124525285	24.201831947900672	31.753924055507156	21.966046872066887
30-31	22.902031002938564	23.7383546445444	31.856831019304032	21.502783333213
32-33	22.50249089080031	24.104362307888543	31.3192816607864	22.07386514052475
34-35	22.33306218322367	24.25381575590714	31.779675293732584	21.633446767136608
36-37	22.587608992301046	24.088652285980395	31.30915908363437	22.01457963808419
38-39	22.531130118359428	23.78475815495988	31.27475849189197	22.40935323478872
40-41	22.20588341471985	23.998632995352665	31.21632126534571	22.579162324581777
42-43	22.572765297920167	23.85166324119043	30.94769369985127	22.627877761038135
44-45	22.55327136991774	24.12457823322439	30.851427388728176	22.47072300812969
46-47	22.365311397449904	24.163084757673627	30.639882170035186	22.831721674841283
48-49	22.550624046361854	24.588100521282076	30.262036898877053	22.599238533479017
50-51	22.414888547678295	24.658134262624124	29.937138098836623	22.989839090860958
52-53	22.355766916641798	24.70999355006402	29.36525891198244	23.568980621311745
54-55	22.15376617875691	24.497128857270752	29.49238774144794	23.85671722252439
56-57	22.4305318232358	24.261757726574796	29.25773860808541	24.049971842103997
58-59	22.58527991836617	24.33997410436231	29.577583426791875	23.497162550479647
60-61	22.892610116626635	24.52312076127399	29.6998416419182	22.884427480181174
62-63	22.19088211439835	24.16193957830441	30.006040725560933	23.641137581736313
64-65	21.817556087159517	24.02614593010103	30.238692318429706	23.917605664309747
66-67	22.531130118359428	24.033125237657455	29.984789922842552	23.450954721140562
68-69	22.13403158497668	24.104362307888543	30.089238870411105	23.672367236723673
70-71	22.14654620542268	24.345750083029692	30.289954129102746	23.217749582444874
72-73	22.360257416115946	24.60326246528396	30.17034323753231	22.866136881067785
74-75	22.343892143225016	24.389310588812894	30.541690532689632	22.72510673527246
76-77	22.106408223088085	23.923198552151195	30.579789320671075	23.390603904089645
78-79	22.16796545964757	24.518307445717834	30.385979774448035	22.927747320186565
80-81	21.956901572510194	24.721188696409747	30.490669387794394	22.831240343285668
82-83	22.065682504079284	24.42757644748432	30.67526003937292	22.831481009063474
84-85	22.113093662307406	24.726724009299325	29.913552852611463	23.246629475781802
86-87	21.628152120024836	25.021058255558177	29.991047233065554	23.359742391351435
88-89	21.920561040061227	24.214346568346674	30.15084930952988	23.714243082062218
90-91	22.444971769904264	23.900037062529783	30.084184889077143	23.570806278488813
92-93	22.251235818769043	24.504589496382792	29.597558686349917	23.64661599849825
94-95	21.663529989362573	24.721429362187557	29.68203237436043	23.93300827408944
96-97	21.683264583142805	24.58665652661523	29.405988727214964	24.324090163026998
98-99	21.649194247097554	24.783640424343943	29.20998671518512	24.35717861337338
100-101	21.909595872080708	25.027436030728357	28.910356379599143	24.15261171759179
102-103	22.11218421565631	24.915706608457498	28.904992142243447	24.06711703364275
104-105	22.431415774792963	24.862639015573595	28.668795771953086	24.03714943768035
106-107	22.08095073066482	24.721548354800824	28.92070505785633	24.276795856678028
108-109	22.10092608637055	24.95836462003504	28.64105007797609	24.299659215618323
110-111	21.542956434680903	25.717785682311543	28.409632407090978	24.329625475916576
112-113	21.63889764651237	25.780785586903836	28.120795839339998	24.4595209272438
114-115	21.675719776371743	25.90737670001516	27.985781363188938	24.431122160424152
116-117	21.579986041250514	25.702149166085054	28.078746600563164	24.63911819210127
118-119	21.546551786380427	25.284894285267324	28.506516167073805	24.662037761278444
120-121	21.73171154346005	25.48591122192593	28.79631679775119	23.98606043686283
122-123	21.415506211822922	25.919499010825565	28.60663589235087	24.05835888500065
124-125	21.287262155176148	25.654509484912225	28.41189693431079	24.646331425600838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	129.5
2	242.0
3	324.5
4	251.5
5	202.5
6	273.5
7	629.0
8	1033.5
9	1168.5
10	1000.5
11	758.5
12	630.0
13	565.5
14	564.0
15	572.5
16	585.5
17	581.0
18	580.0
19	550.5
20	531.0
21	572.5
22	565.5
23	566.5
24	630.0
25	677.5
26	715.5
27	818.0
28	960.5
29	1201.5
30	1643.5
31	2073.0
32	2532.0
33	3049.0
34	3451.5
35	3818.0
36	4249.0
37	4669.5
38	4794.0
39	4728.5
40	4648.5
41	4490.0
42	4508.5
43	4847.0
44	5350.0
45	5822.0
46	6365.0
47	7009.5
48	7691.5
49	8315.0
50	9149.0
51	9119.5
52	8171.5
53	7895.0
54	7883.0
55	7812.5
56	7506.5
57	6690.0
58	5795.0
59	4950.5
60	4180.0
61	3382.0
62	2676.5
63	2094.0
64	1590.5
65	1305.5
66	1036.0
67	820.5
68	677.5
69	557.0
70	438.5
71	353.5
72	301.0
73	277.0
74	240.5
75	187.5
76	142.5
77	123.5
78	107.5
79	71.5
80	51.0
81	48.5
82	37.5
83	32.0
84	26.0
85	15.0
86	9.0
87	3.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.043319840005390915
2	0.0019253262224618183
3	0.0014439946668463638
4	0.0048133155561545455
5	0.003369320889308182
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.8133155561545457E-4
14-15	0.0
16-17	0.0
18-19	2.4066577780772728E-4
20-21	4.8133155561545457E-4
22-23	0.0
24-25	0.0
26-27	4.8133155561545457E-4
28-29	0.0
30-31	0.0012033288890386364
32-33	0.0
34-35	0.0
36-37	7.219973334231819E-4
38-39	0.0
40-41	0.001684660444654091
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0014439946668463638
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	2.4066577780772728E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.4066577780772728E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	4.8133155561545457E-4
100-101	4.8133155561545457E-4
102-103	2.4066577780772728E-4
104-105	7.219973334231819E-4
106-107	4.8133155561545457E-4
108-109	4.8133155561545457E-4
110-111	0.0
112-113	7.219973334231819E-4
114-115	7.219973334231819E-4
116-117	9.626631112309091E-4
118-119	0.0021659920002695455
120-121	0.0014439946668463638
122-123	0.0038506524449236365
124-125	0.0028879893336927276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	207757.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.88001197880321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10362951621774	50.531408010628
2	13.946016644588246	17.817434206257722
3	3.312158816125019	6.3474223454929435
4	1.1951115837994801	3.0537496915646902
5	0.6557079114029072	2.0943314617506865
6	0.4156977611883792	1.5932866778565202
7	0.26673758311273993	1.1927440003107188
8	0.20664274206407982	1.0560272670708928
9	0.12889468429011838	0.7410414578811136
>10	0.6995466931352131	7.911043374208317
>50	0.04329108196263113	1.7849624839884017
>100	0.023613317434162437	2.9596437806695013
>500	0.0	0.0
>1k	0.0029516646792703046	2.9169052423204835
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1967	0.9467791698955992	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1625	0.7821637778751137	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1049	0.5049168018406118	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.22574449958364823	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	399	0.1920512906905664	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	385	0.18531264891195	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	327	0.15739541868625365	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	286	0.13766082490602	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	257	0.12370220979317183	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	246	0.11840756268140183	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	241	0.11600090490332456	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	2.4066577780772728E-4
12-13	0.0	0.0	0.0	0.0	4.8133155561545457E-4
14-15	0.0	0.0	0.0	0.0	4.8133155561545457E-4
16-17	0.0	0.0	0.0	0.0	4.8133155561545457E-4
18-19	0.0	0.0	0.0	0.0	4.8133155561545457E-4
20-21	0.0	0.0	0.0	4.8133155561545457E-4	4.8133155561545457E-4
22-23	0.0	0.0	0.0	0.0019253262224618183	4.8133155561545457E-4
24-25	0.0	0.0	0.0	0.007219973334231819	4.8133155561545457E-4
26-27	0.0	0.0	0.0	0.01780926755777182	4.8133155561545457E-4
28-29	0.0	0.0	0.0	0.035137203559928186	4.8133155561545457E-4
30-31	0.0	0.0	0.0	0.06546109156370182	4.8133155561545457E-4
32-33	0.0	0.0	0.0	0.11166892090278546	4.8133155561545457E-4
34-35	0.0	0.0	0.0	0.1665407182429473	4.8133155561545457E-4
36-37	0.0	0.0	0.0	0.25967837425453777	4.8133155561545457E-4
38-39	0.0	0.0	0.0	0.37519794760224684	4.8133155561545457E-4
40-41	0.0	0.0	0.0	0.5010661493956883	4.8133155561545457E-4
42-43	0.0	0.0	0.0	0.6052744311864342	4.8133155561545457E-4
44-45	0.0	0.0	0.0	0.7207940045341432	4.8133155561545457E-4
46-47	0.0	0.0	0.0	0.8618241503294715	4.8133155561545457E-4
48-49	0.0	0.0	0.0	1.010555601014647	4.8133155561545457E-4
50-51	0.0	0.0	0.0	1.1426811130310892	4.8133155561545457E-4
52-53	0.0	0.0	0.0	1.2760099539365701	4.8133155561545457E-4
54-55	0.0	0.0	0.0	1.4033221503968578	4.8133155561545457E-4
56-57	0.0	0.0	0.0	1.5419456384141088	4.8133155561545457E-4
58-59	0.0	0.0	0.0	1.707764359323633	4.8133155561545457E-4
60-61	0.0	0.0	0.0	1.8629937860096168	4.8133155561545457E-4
62-63	0.0	0.0	0.0	2.0018579398046756	4.8133155561545457E-4
64-65	0.0	0.0	0.0	2.1527553824901204	4.8133155561545457E-4
66-67	0.0	0.0	0.0	2.314001453621298	4.8133155561545457E-4
68-69	0.0	0.0	0.0	2.497870107866402	4.8133155561545457E-4
70-71	0.0	0.0	0.0	2.687514740778891	4.8133155561545457E-4
72-73	0.0	0.0	0.0	2.8810100261363036	4.8133155561545457E-4
74-75	0.0	0.0	0.0	3.058862035936214	4.8133155561545457E-4
76-77	0.0	0.0	0.0	3.2461000110706255	4.8133155561545457E-4
78-79	0.0	0.0	0.0	3.4540352430965022	4.8133155561545457E-4
80-81	0.0	0.0	0.0	3.6706344431234568	7.219973334231819E-4
82-83	0.0	0.0	0.0	3.9035989160413367	9.626631112309091E-4
84-85	0.0	0.0	0.0	4.121642110735138	9.626631112309091E-4
86-87	0.0	0.0	0.0	4.348830604985633	9.626631112309091E-4
88-89	0.0	0.0	0.0	4.589977714348975	9.626631112309091E-4
90-91	0.0	0.0	0.0	4.84989675438132	9.626631112309091E-4
92-93	0.0	0.0	0.0	5.119442425525975	9.626631112309091E-4
94-95	0.0	0.0	0.0	5.372141492224088	9.626631112309091E-4
96-97	0.0	0.0	0.0	5.637114513590396	9.626631112309091E-4
98-99	0.0	0.0	0.0	5.948295364295788	9.626631112309091E-4
100-101	0.0	0.0	0.0	6.27632281944772	9.626631112309091E-4
102-103	0.0	0.0	0.0	6.589188330597766	9.626631112309091E-4
104-105	0.0	0.0	0.0	6.9367097137521245	9.626631112309091E-4
106-107	0.0	0.0	0.0	7.294098393796599	9.626631112309091E-4
108-109	0.0	0.0	0.0	7.6341591378389175	9.626631112309091E-4
110-111	0.0	0.0	0.0	8.002137112106933	9.626631112309091E-4
112-113	0.0	0.0	0.0	8.4091029423798	9.626631112309091E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACCTC	40	1.7650511E-4	59.524082	3
GTAAGTA	180	1.4551915E-11	36.375828	2
GGTATCA	1735	0.0	34.324356	1
CTTACAC	70	0.002778237	34.01376	3
GTATCAA	2405	0.0	33.428783	1
ATGTAAG	125	1.2673299E-6	33.333485	3
ATATGTA	130	1.7151142E-6	32.066868	1
TAAGGTG	100	4.4220156E-4	29.754875	5
ATAAGAG	105	5.875307E-4	28.344799	3
TGTAAGT	190	3.655623E-8	28.209202	1
CTTTACC	85	0.007165905	28.024828	1
TATCAAC	2920	0.0	27.519695	2
TTATCCT	130	6.336048E-5	27.47265	4
TATGTAA	130	6.336048E-5	27.47265	2
CTAGAAC	45	0.009525662	26.442411	74-75
CTAAATT	45	0.009525662	26.442411	72-73
CAACGCA	3150	0.0	26.259857	5
ATCAACG	3070	0.0	26.175083	3
TCAACGC	3200	0.0	26.041784	4
AACGCAG	3275	0.0	25.978155	6
>>END_MODULE