FastQCFastQC Report
Sun 1 Jan 2017
ERR1142853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425880
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8660.2033436648821264No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6280.147459378228609No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4290.10073260073260075No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC700.002784652634.0058863
GTGTTAA1454.023859E-628.7561931
CATGGGG18350.027.8903874
GGGCTAT2052.2657005E-623.2098716
GTACATG46850.022.5042761
ATGGGGG11300.022.1188745
CTAGACA1350.0025500922.0408544
TACATGG47000.022.0314732
ACATGGG45300.021.8073063
GGTATCA23250.020.7521671
GTATCAA27900.020.2824671
ATGGGGC4800.019.8367675
TATATAC1500.00469639719.8367673
GTTAAGT1500.00469639719.8367673
GTACACT1550.00567670919.196874
TAGACAG2501.2567096E-519.0432955
TTTAGGG2601.7619754E-518.3108622
ATGGGTA2952.5676727E-618.1556845
ATTTAGG2301.4212848E-418.1289061
GGCTATT2351.6938524E-417.7181287
Produced by FastQC (version 0.11.5)