##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142853_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425880
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32581478350709	33.0	33.0	33.0	27.0	33.0
2	31.37280454588147	33.0	33.0	33.0	27.0	33.0
3	31.45109655301963	33.0	33.0	33.0	27.0	33.0
4	31.404149056072132	33.0	33.0	33.0	27.0	33.0
5	31.48537616229924	33.0	33.0	33.0	27.0	33.0
6	34.8503498638114	37.0	37.0	37.0	27.0	37.0
7	34.81033154879309	37.0	37.0	37.0	27.0	37.0
8	34.78762327416174	37.0	37.0	37.0	27.0	37.0
9	34.75470085470086	37.0	37.0	37.0	27.0	37.0
10-11	34.80383676152907	37.0	37.0	37.0	27.0	37.0
12-13	34.7484690523152	37.0	37.0	37.0	27.0	37.0
14-15	34.757176904292294	37.0	37.0	37.0	27.0	37.0
16-17	34.76753310791773	37.0	37.0	37.0	27.0	37.0
18-19	34.758644453836766	37.0	37.0	37.0	27.0	37.0
20-21	34.76401568516953	37.0	37.0	37.0	27.0	37.0
22-23	34.82212947309101	37.0	37.0	37.0	27.0	37.0
24-25	34.888554287592754	37.0	37.0	37.0	27.0	37.0
26-27	34.916272189349115	37.0	37.0	37.0	27.0	37.0
28-29	34.986932938856015	37.0	37.0	37.0	27.0	37.0
30-31	34.98704212454213	37.0	37.0	37.0	27.0	37.0
32-33	35.034944115713344	37.0	37.0	37.0	27.0	37.0
34-35	35.05083943833944	37.0	37.0	37.0	33.0	37.0
36-37	35.034887761810836	37.0	37.0	37.0	27.0	37.0
38-39	35.022224570301496	37.0	37.0	37.0	27.0	37.0
40-41	34.99267281863436	37.0	37.0	37.0	27.0	37.0
42-43	34.98038297172913	37.0	37.0	37.0	27.0	37.0
44-45	34.9755905419367	37.0	37.0	37.0	27.0	37.0
46-47	34.974883770076076	37.0	37.0	37.0	27.0	37.0
48-49	34.933649149995304	37.0	37.0	37.0	27.0	37.0
50-51	34.91471189067343	37.0	37.0	37.0	27.0	37.0
52-53	34.8861040668733	37.0	37.0	37.0	27.0	37.0
54-55	34.87175260636799	37.0	37.0	37.0	27.0	37.0
56-57	34.831740161547856	37.0	37.0	37.0	27.0	37.0
58-59	34.78016342631727	37.0	37.0	37.0	27.0	37.0
60-61	34.75685991359069	37.0	37.0	37.0	27.0	37.0
62-63	34.71466258100874	37.0	37.0	37.0	27.0	37.0
64-65	34.66630154034	37.0	37.0	37.0	27.0	37.0
66-67	34.64087536395229	37.0	37.0	37.0	27.0	37.0
68-69	34.55765356438434	37.0	37.0	37.0	27.0	37.0
70-71	34.47975955668264	37.0	37.0	37.0	27.0	37.0
72-73	34.44472621395698	37.0	37.0	37.0	27.0	37.0
74-75	34.39896801916033	37.0	37.0	37.0	27.0	37.0
76-77	34.31840189724805	37.0	37.0	37.0	27.0	37.0
78-79	34.27271179675026	37.0	37.0	37.0	27.0	37.0
80-81	34.21655630694092	37.0	37.0	37.0	27.0	37.0
82-83	34.1454987320372	37.0	37.0	37.0	27.0	37.0
84-85	34.06429628064244	37.0	37.0	37.0	27.0	37.0
86-87	33.99268808114962	37.0	37.0	37.0	27.0	37.0
88-89	33.93800014088475	37.0	37.0	37.0	22.0	37.0
90-91	33.85352211890674	37.0	37.0	37.0	22.0	37.0
92-93	33.79878369493754	37.0	37.0	37.0	22.0	37.0
94-95	33.749902554710246	37.0	33.0	37.0	22.0	37.0
96-97	33.64380576688269	37.0	33.0	37.0	22.0	37.0
98-99	33.525184324222785	37.0	33.0	37.0	22.0	37.0
100-101	33.425348689771766	37.0	33.0	37.0	22.0	37.0
102-103	33.366512867474405	37.0	33.0	37.0	22.0	37.0
104-105	33.21700713816098	37.0	33.0	37.0	14.0	37.0
106-107	33.14397600262985	37.0	33.0	37.0	14.0	37.0
108-109	33.03569550108011	37.0	33.0	37.0	14.0	37.0
110-111	32.89839508781816	37.0	33.0	37.0	14.0	37.0
112-113	32.76767986287217	37.0	33.0	37.0	14.0	37.0
114-115	32.65085117873579	37.0	33.0	37.0	14.0	37.0
116-117	32.54634521461445	37.0	33.0	37.0	14.0	37.0
118-119	32.410706067436834	37.0	33.0	37.0	14.0	37.0
120-121	32.33319127453743	37.0	33.0	37.0	14.0	37.0
122-123	32.145121865314174	37.0	33.0	37.0	14.0	37.0
124-125	30.128617216117217	35.0	23.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	303.0
1	36.0
2	23.0
3	23.0
4	17.0
5	23.0
6	14.0
7	12.0
8	9.0
9	23.0
10	25.0
11	11.0
12	9.0
13	17.0
14	34.0
15	175.0
16	247.0
17	429.0
18	859.0
19	1678.0
20	2671.0
21	3327.0
22	3895.0
23	4488.0
24	4544.0
25	5090.0
26	5725.0
27	6801.0
28	8237.0
29	9473.0
30	11284.0
31	13765.0
32	17386.0
33	23167.0
34	34533.0
35	65604.0
36	201923.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.87244014168095	20.029238735213166	12.18977913473087	23.908541988375017
2	17.497854329551465	23.21979895361825	35.673387807889014	23.608958908941275
3	19.77443255321651	27.940962013407034	27.984946489474304	24.299658943902152
4	14.649591629342467	19.733524031766343	33.80725657721405	31.809627761677145
5	15.453428892768573	37.87499470595709	30.88426986913126	15.787306532143075
6	29.417586421712095	36.26949704385439	18.28860914230638	16.02430739212713
7	26.14010553159484	33.54964568178346	21.484023362673778	18.826225423947914
8	25.7410362404499	33.9975418253944	20.318246658786094	19.943175275369608
9	26.550993881815383	18.65870025596247	19.74037085177446	35.04993501044768
10-11	25.226004401988767	27.61055208177875	25.281822465654262	21.881621050578218
12-13	25.92538185185664	26.51744760664646	24.92881226062645	22.628358280870447
14-15	24.602136434206923	26.629034020992954	23.149600931766443	25.619228613033684
16-17	24.108812388421583	28.328195388425108	24.101760691254366	23.46123153189894
18-19	24.167781306491083	27.49160200188529	25.47843318860646	22.862183503017167
20-21	26.00994786304294	26.066245433902658	24.79996144968008	23.123845253374327
22-23	25.690061654831663	26.028540334200684	24.580603761344324	23.700794249623325
24-25	24.667715624974296	26.628225752435657	24.783813923100155	23.920244699489896
26-27	24.395208919280094	26.578956337980163	24.83548436763588	24.19035037510387
28-29	24.246552478031305	26.27486611907527	25.305728081847207	24.172853321046222
30-31	24.733562696073992	26.16275037818993	25.20361134652071	23.90007557921536
32-33	24.132682147034103	26.497875416541778	24.945959765149777	24.42348267127434
34-35	24.271969060227935	26.425292558350034	25.170421483863002	24.132316897559026
36-37	24.434443387921238	26.53470443665308	24.78270425799748	24.248147917428206
38-39	24.391806280691775	26.467739698494825	24.839268545530196	24.301185475283205
40-41	24.24886498026206	26.529014419629316	25.03567876649041	24.186441833618215
42-43	24.29874568384273	26.42105473539913	25.16203592124461	24.118163659513534
44-45	24.410443075924633	26.571505160166197	24.838046731894742	24.180005032014428
46-47	24.327120327755146	26.493972150217193	24.96252799379287	24.21637952823479
48-49	24.40632857675204	26.649002387498953	25.039938590376416	23.90473044537259
50-51	24.005219752767115	26.890543900589574	24.94583330884125	24.158403037802064
52-53	24.049888775495806	26.69493916748576	24.884072556940833	24.371099500077598
54-55	24.045469506747544	26.56662904970142	25.0	24.38790144355104
56-57	24.340321084686465	26.255026866773484	24.661006090428938	24.74364595811111
58-59	24.211215627906103	26.373267213822082	25.051699955677353	24.36381720259446
60-61	24.32615109731871	26.431921571393307	25.182847268751985	24.059080062535998
62-63	23.819141794685798	26.731359093522254	25.01760544751931	24.43189366427264
64-65	23.806344142019626	26.435531709542076	25.130283797080562	24.627840351357737
66-67	24.453660795944806	26.21370179930454	24.875685054698575	24.456952350052077
68-69	24.38920022053801	26.091198816900548	24.81969606664104	24.699904895920398
70-71	24.261573116135583	26.216799593694745	24.969668184041232	24.551959106128436
72-73	24.497578856166776	26.279785241779337	24.999206471431208	24.22342943062268
74-75	24.47287199957672	26.272508686015954	25.05076395788385	24.203855356523476
76-77	24.400786917323117	26.088449413045755	25.190761600033866	24.320002069597262
78-79	24.347535709750396	26.268595643810233	25.073018620336097	24.310850026103275
80-81	24.004030793000908	26.266472103690617	25.294519509014606	24.434977594293862
82-83	24.455100466586394	26.16623080975316	25.160271485550872	24.218397238109574
84-85	24.30524767181089	26.301863139289157	24.898422864292947	24.494466324607007
86-87	23.971566990428258	26.444580325015878	25.0506808400555	24.533171844500366
88-89	24.431897015974847	25.95659770806926	25.149271807558716	24.462233468397176
90-91	24.516777694808823	25.980702858117205	25.161662070709152	24.340857376364823
92-93	24.110077459215436	26.42127135655346	25.09492781487871	24.373723369352398
94-95	23.505894111767645	26.583296587000614	25.433871299644295	24.476938001587442
96-97	23.965646601704265	26.359871015995996	25.058976548149086	24.615505834150657
98-99	23.817124682263074	26.65496681185622	25.001382104162428	24.52652640171828
100-101	23.895372422672835	26.896859879239692	24.785964994796842	24.42180270329063
102-103	23.987163446160793	26.928893299968017	24.976363619259065	24.10757963461213
104-105	23.8973939203628	26.97308301608527	25.052306237584986	24.077216825966946
106-107	23.778973972378804	26.947661324561412	25.0640664527002	24.209298250359584
108-109	23.881932757791397	26.927224638933872	24.969394199186432	24.2214484040883
110-111	23.639859358647207	27.367090393819304	24.87999623702066	24.113054010512826
112-113	23.75052923742767	27.246906901256057	24.694688808392527	24.30787505292374
114-115	23.517387704409025	27.4243286727762	24.908498146440603	24.149785476374173
116-117	23.775537084338826	27.338615975877655	24.82936076732382	24.0564861724597
118-119	23.5825824342537	27.089992638013722	24.993002584927453	24.33442234280513
120-121	23.59422934689179	27.20953011437595	25.165884523441367	24.03035601529089
122-123	23.627991962252214	27.30000576140494	25.042828403020856	24.02917387332199
124-125	23.729983791782033	27.19334357422964	24.740821420253923	24.335851213734404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	580.0
1	471.0
2	245.0
3	112.0
4	90.0
5	69.0
6	57.5
7	78.0
8	88.5
9	79.0
10	81.5
11	82.0
12	83.0
13	94.0
14	107.5
15	127.5
16	165.0
17	201.0
18	244.5
19	275.5
20	307.0
21	392.0
22	501.0
23	586.5
24	681.5
25	826.5
26	1037.0
27	1299.5
28	1561.0
29	2058.0
30	2872.5
31	3610.5
32	4453.5
33	5399.0
34	6251.5
35	7118.0
36	8000.0
37	9016.0
38	9505.0
39	9536.5
40	9468.0
41	9311.0
42	9654.0
43	10610.5
44	11961.0
45	13095.5
46	14404.5
47	15779.5
48	17367.0
49	18793.0
50	19546.0
51	18918.0
52	17530.0
53	17280.5
54	17041.0
55	16395.0
56	15566.5
57	14219.0
58	13000.5
59	11902.0
60	10468.0
61	8879.5
62	7292.5
63	5762.0
64	4418.5
65	3535.5
66	2818.0
67	2156.0
68	1787.5
69	1574.5
70	1293.0
71	1082.5
72	935.0
73	870.5
74	720.0
75	615.5
76	513.0
77	348.5
78	251.5
79	176.5
80	136.5
81	106.5
82	82.0
83	57.5
84	40.0
85	27.0
86	16.5
87	9.0
88	6.5
89	3.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09791490560721329
2	0.14205879590494974
3	0.17140978679440216
4	0.18221095144172067
5	0.20522212829905137
6	0.11622992392223161
7	0.09744528975298207
8	0.08476566168873861
9	0.10026298487836949
10-11	0.09169249553864939
12-13	0.09873673335211797
14-15	0.10601577909270217
16-17	0.10578097116558656
18-19	0.11294261294261296
20-21	0.10848126232741617
22-23	0.10495914342068188
24-25	0.08887480041326196
26-27	0.10906828214520521
28-29	0.11787357941204096
30-31	0.11705175166713629
32-33	0.11799098337559877
34-35	0.12620926082464543
36-37	0.11282520897905512
38-39	0.11294261294261296
40-41	0.13078801540340002
42-43	0.13818446510754204
44-45	0.14158918005071852
46-47	0.13207945900253593
48-49	0.12667887667887667
50-51	0.1344275382736921
52-53	0.14417206724899032
54-55	0.12679628064243448
56-57	0.1269136846059923
58-59	0.13841927303465765
60-61	0.1232741617357002
62-63	0.13748004132619518
64-65	0.13114022729407343
66-67	0.12890955198647508
68-69	0.1306706114398422
70-71	0.13712782943552174
72-73	0.13254907485676717
74-75	0.1473419742650512
76-77	0.15884756269371653
78-79	0.15168592091669014
80-81	0.1534469803700573
82-83	0.1559124636047713
84-85	0.1668310322156476
86-87	0.157321311167465
88-89	0.152390344698037
90-91	0.16143044989198835
92-93	0.16166525781910396
94-95	0.15673429134967595
96-97	0.1669484361792054
98-99	0.18866816943740022
100-101	0.15438621207851977
102-103	0.16154785385554615
104-105	0.17364046210200057
106-107	0.17270123039353807
108-109	0.1680050718512257
110-111	0.16084343007419932
112-113	0.17375786606555838
114-115	0.1765755611909458
116-117	0.16765285996055226
118-119	0.16976613130459284
120-121	0.15485582793275102
122-123	0.14945524560909176
124-125	0.1847938386399925
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	425880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.62884307805166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.48439767747573	62.08632904539147
2	9.699487414190655	14.089250986855802
3	2.3250310740560804	5.065929510876389
4	0.8714297313293047	2.531637328410591
5	0.4163683743425887	1.5120176661395672
6	0.2500928414608957	1.0898372242444467
7	0.17069357744882274	0.8678093935673262
8	0.12412046635693168	0.7211780699049744
9	0.08574797598028225	0.5604998662559215
>10	0.5117156274466049	7.090360661430534
>50	0.046250459935028576	2.2705448810517503
>100	0.013912739978289552	1.706595496749224
>500	7.520399988264623E-4	0.40800986912199405
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	866	0.2033436648821264	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	628	0.147459378228609	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	429	0.10073260073260075	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	2.3480792711561943E-4	0.0
20-21	0.0	0.0	0.0	9.392317084624777E-4	0.0
22-23	0.0	0.0	0.0	0.002700291161829623	0.0
24-25	0.0	0.0	0.0	0.007161641777026393	0.0
26-27	0.0	0.0	0.0	0.01174039635578097	0.0
28-29	0.0	0.0	0.0	0.021367521367521368	0.0
30-31	0.0	1.1740396355780971E-4	0.0	0.039799943646097485	0.0
32-33	0.0	2.3480792711561943E-4	0.0	0.06856391471776087	0.0
34-35	0.0	2.3480792711561943E-4	0.0	0.10131962055038979	0.0
36-37	0.0	2.3480792711561943E-4	0.0	0.15321217244294166	0.0
38-39	0.0	2.3480792711561943E-4	0.0	0.22060204752512447	0.0
40-41	0.0	2.3480792711561943E-4	0.0	0.2976190476190476	0.0
42-43	0.0	2.3480792711561943E-4	0.0	0.36829623368084907	1.1740396355780971E-4
44-45	0.0	2.3480792711561943E-4	0.0	0.4335728374189913	2.3480792711561943E-4
46-47	0.0	2.3480792711561943E-4	0.0	0.5203343664882126	2.3480792711561943E-4
48-49	0.0	2.3480792711561943E-4	0.0	0.607682915375223	2.3480792711561943E-4
50-51	0.0	2.3480792711561943E-4	0.0	0.6905701136470368	2.3480792711561943E-4
52-53	0.0	2.3480792711561943E-4	0.0	0.7704048088663473	2.3480792711561943E-4
54-55	0.0	2.3480792711561943E-4	0.0	0.848948060486522	2.3480792711561943E-4
56-57	0.0	2.3480792711561943E-4	0.0	0.9441626749319056	2.3480792711561943E-4
58-59	0.0	2.3480792711561943E-4	0.0	1.0478303747534516	2.3480792711561943E-4
60-61	0.0	2.3480792711561943E-4	0.0	1.1573682727528882	2.3480792711561943E-4
62-63	0.0	2.3480792711561943E-4	0.0	1.2550483704329858	2.3480792711561943E-4
64-65	0.0	2.3480792711561943E-4	0.0	1.3519066403681788	2.3480792711561943E-4
66-67	0.0	2.3480792711561943E-4	0.0	1.4681365642904105	2.3480792711561943E-4
68-69	0.0	2.3480792711561943E-4	0.0	1.5965765004226542	2.3480792711561943E-4
70-71	0.0	2.3480792711561943E-4	0.0	1.7457969381046303	2.3480792711561943E-4
72-73	0.0	2.3480792711561943E-4	0.0	1.8802244763783225	2.3480792711561943E-4
74-75	0.0	2.3480792711561943E-4	0.0	2.0042030618953697	2.3480792711561943E-4
76-77	0.0	2.3480792711561943E-4	0.0	2.1307645346106883	2.3480792711561943E-4
78-79	0.0	2.3480792711561943E-4	0.0	2.290081713158636	2.3480792711561943E-4
80-81	0.0	2.3480792711561943E-4	0.0	2.4605522682445757	2.3480792711561943E-4
82-83	0.0	2.3480792711561943E-4	0.0	2.6273833004602234	2.3480792711561943E-4
84-85	0.0	2.3480792711561943E-4	0.0	2.7857612472997086	2.3480792711561943E-4
86-87	0.0	2.3480792711561943E-4	0.0	2.948483140790833	2.3480792711561943E-4
88-89	0.0	2.3480792711561943E-4	0.0	3.131868131868132	2.3480792711561943E-4
90-91	0.0	2.3480792711561943E-4	0.0	3.330163426317273	2.3480792711561943E-4
92-93	0.0	2.3480792711561943E-4	0.0	3.5367944021790176	2.3480792711561943E-4
94-95	0.0	2.3480792711561943E-4	0.0	3.728397670705363	2.3480792711561943E-4
96-97	0.0	2.3480792711561943E-4	0.0	3.9281018127171974	2.3480792711561943E-4
98-99	0.0	2.3480792711561943E-4	0.0	4.155865502019348	7.044237813468582E-4
100-101	0.0	2.3480792711561943E-4	0.0	4.392904104442566	7.044237813468582E-4
102-103	0.0	2.3480792711561943E-4	0.0	4.639452427913966	7.044237813468582E-4
104-105	0.0	2.3480792711561943E-4	0.0	4.884239691932	7.044237813468582E-4
106-107	0.0	2.3480792711561943E-4	0.0	5.145698318775242	7.044237813468582E-4
108-109	0.0	2.3480792711561943E-4	0.0	5.415727434958204	7.044237813468582E-4
110-111	0.0	2.3480792711561943E-4	0.0	5.698905795059641	7.044237813468582E-4
112-113	0.0	2.3480792711561943E-4	0.0	5.999107729876961	7.044237813468582E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACAC	70	0.0027846526	34.005886	3
GTGTTAA	145	4.023859E-6	28.756193	1
CATGGGG	1835	0.0	27.890387	4
GGGCTAT	205	2.2657005E-6	23.209871	6
GTACATG	4685	0.0	22.504276	1
ATGGGGG	1130	0.0	22.118874	5
CTAGACA	135	0.00255009	22.040854	4
TACATGG	4700	0.0	22.031473	2
ACATGGG	4530	0.0	21.807306	3
GGTATCA	2325	0.0	20.752167	1
GTATCAA	2790	0.0	20.282467	1
ATGGGGC	480	0.0	19.836767	5
TATATAC	150	0.004696397	19.836767	3
GTTAAGT	150	0.004696397	19.836767	3
GTACACT	155	0.005676709	19.19687	4
TAGACAG	250	1.2567096E-5	19.043295	5
TTTAGGG	260	1.7619754E-5	18.310862	2
ATGGGTA	295	2.5676727E-6	18.155684	5
ATTTAGG	230	1.4212848E-4	18.128906	1
GGCTATT	235	1.6938524E-4	17.718128	7
>>END_MODULE