FastQCFastQC Report
Sun 1 Jan 2017
ERR1142853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425880
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20330.47736451582605427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.4167840706302245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.25523621677467834No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6040.14182398797783413No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5890.13830186907109984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.11223818916126609No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24850.032.1028751
GTATCAA32900.028.9527381
TACAACG850.00720174528.0028722
TACGGCA850.00720587527.9995845
CATGGGT5200.025.1756614
ATGGGTA1951.4613797E-624.4098975
GAGTACT19000.024.26937712-13
CAACGCA40550.024.2104915
TATCAAC40300.024.0682262
ATCAACG39800.023.9220523
TCAACGC40650.023.8609984
CTAACGC1250.001630869723.8024433
ATGGGAC2502.001434E-823.7996485
AACGCAG43050.023.3573786
CATGGGG16500.022.7205124
GTACATA1602.59744E-422.3252751
ACATGGG38300.021.7515773
ACGCAGA45950.021.6242717
GTACTTT21700.021.24968514-15
CGCAGAG47200.021.1776528
Produced by FastQC (version 0.11.5)