##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142853_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425880
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.061716915563068	33.0	14.0	33.0	14.0	33.0
2	30.098729689114304	33.0	27.0	33.0	27.0	33.0
3	29.772287968441816	33.0	27.0	33.0	27.0	33.0
4	31.082276697661314	33.0	33.0	33.0	27.0	33.0
5	31.829294636986944	33.0	33.0	33.0	27.0	33.0
6	34.50016671362825	37.0	33.0	37.0	27.0	37.0
7	35.7013431013431	37.0	37.0	37.0	33.0	37.0
8	35.553590213205595	37.0	37.0	37.0	33.0	37.0
9	35.93241288625904	37.0	37.0	37.0	33.0	37.0
10-11	35.94996595285056	37.0	37.0	37.0	33.0	37.0
12-13	36.01005447543909	37.0	37.0	37.0	33.0	37.0
14-15	36.086593641401336	37.0	37.0	37.0	37.0	37.0
16-17	36.10920094862402	37.0	37.0	37.0	37.0	37.0
18-19	36.11994106321029	37.0	37.0	37.0	37.0	37.0
20-21	36.06841363764441	37.0	37.0	37.0	37.0	37.0
22-23	36.11141401333709	37.0	37.0	37.0	37.0	37.0
24-25	36.14551164647318	37.0	37.0	37.0	37.0	37.0
26-27	36.09549168779938	37.0	37.0	37.0	37.0	37.0
28-29	36.10410679064525	37.0	37.0	37.0	37.0	37.0
30-31	36.05920799286184	37.0	37.0	37.0	37.0	37.0
32-33	36.0102364515826	37.0	37.0	37.0	37.0	37.0
34-35	35.9542007138161	37.0	37.0	37.0	37.0	37.0
36-37	35.89540363482671	37.0	37.0	37.0	35.0	37.0
38-39	35.84119470273316	37.0	37.0	37.0	33.0	37.0
40-41	35.76895486991641	37.0	37.0	37.0	33.0	37.0
42-43	35.682761576030806	37.0	37.0	37.0	33.0	37.0
44-45	35.603485723678034	37.0	37.0	37.0	33.0	37.0
46-47	35.478677092138625	37.0	37.0	37.0	33.0	37.0
48-49	35.26123203719358	37.0	37.0	37.0	33.0	37.0
50-51	35.22358410819949	37.0	37.0	37.0	33.0	37.0
52-53	35.200213675213675	37.0	37.0	37.0	33.0	37.0
54-55	35.12118906734291	37.0	37.0	37.0	33.0	37.0
56-57	35.105085939701326	37.0	37.0	37.0	33.0	37.0
58-59	35.064832816755896	37.0	37.0	37.0	33.0	37.0
60-61	35.036203860242324	37.0	37.0	37.0	33.0	37.0
62-63	35.010460693153	37.0	37.0	37.0	33.0	37.0
64-65	34.9898692119846	37.0	37.0	37.0	27.0	37.0
66-67	34.95813609467456	37.0	37.0	37.0	27.0	37.0
68-69	34.887934394665166	37.0	37.0	37.0	27.0	37.0
70-71	34.86303536207382	37.0	37.0	37.0	27.0	37.0
72-73	34.8460270498732	37.0	37.0	37.0	27.0	37.0
74-75	34.79734080022541	37.0	37.0	37.0	27.0	37.0
76-77	34.75068798722645	37.0	37.0	37.0	27.0	37.0
78-79	34.6664435991359	37.0	37.0	37.0	27.0	37.0
80-81	34.575	37.0	37.0	37.0	27.0	37.0
82-83	34.58808819385743	37.0	37.0	37.0	27.0	37.0
84-85	34.55301258570489	37.0	37.0	37.0	27.0	37.0
86-87	34.487838123415045	37.0	37.0	37.0	27.0	37.0
88-89	34.41393819855358	37.0	37.0	37.0	27.0	37.0
90-91	34.36951958298112	37.0	37.0	37.0	27.0	37.0
92-93	34.26945735888044	37.0	37.0	37.0	27.0	37.0
94-95	34.23004250023481	37.0	37.0	37.0	27.0	37.0
96-97	34.167632901286744	37.0	37.0	37.0	27.0	37.0
98-99	34.084650605804455	37.0	37.0	37.0	22.0	37.0
100-101	34.024540950502484	37.0	37.0	37.0	22.0	37.0
102-103	33.94104325162017	37.0	37.0	37.0	22.0	37.0
104-105	33.80582558467174	37.0	35.0	37.0	22.0	37.0
106-107	33.73789095519865	37.0	33.0	37.0	22.0	37.0
108-109	33.65294918756457	37.0	33.0	37.0	22.0	37.0
110-111	33.610559312482394	37.0	33.0	37.0	22.0	37.0
112-113	33.5490725086879	37.0	33.0	37.0	22.0	37.0
114-115	33.40389663755049	37.0	33.0	37.0	22.0	37.0
116-117	33.35898140321217	37.0	33.0	37.0	22.0	37.0
118-119	33.185808208885135	37.0	33.0	37.0	14.0	37.0
120-121	33.09443505212736	37.0	33.0	37.0	14.0	37.0
122-123	33.01308936789706	37.0	33.0	37.0	14.0	37.0
124-125	31.369774349582045	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	11.0
16	7.0
17	10.0
18	68.0
19	218.0
20	1006.0
21	2424.0
22	3736.0
23	4944.0
24	4356.0
25	3375.0
26	3343.0
27	3620.0
28	4654.0
29	6150.0
30	8058.0
31	11018.0
32	15263.0
33	22276.0
34	37397.0
35	79064.0
36	214881.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.334977567942126	17.097691024827945	12.686914241420618	26.880417165809316
2	17.02632258670486	20.76549181675159	37.44076830957804	24.767417286965507
3	20.050390136119116	25.31341208846686	28.601685463173638	26.034512312240388
4	14.222748225326578	17.46236377341477	35.31922987509775	32.995658126160905
5	15.651761457161708	34.25536960896426	32.63374417954817	17.45912475432586
6	29.648492533107916	34.37588052972668	18.995726495726494	16.979900441438904
7	26.090213205597824	31.775617544848313	22.708274631351554	19.42589461820231
8	26.090917629379167	31.276415891800507	21.423405654174886	21.20926082464544
9	26.865079365079364	15.832863717479102	21.121677467831315	36.180379449610214
10-11	25.321804264111957	24.99683009298394	26.730064806987887	22.95130083591622
12-13	26.678649754154947	24.00006574652835	26.612433607746823	22.708850891569888
14-15	24.900734715729115	24.003414115276875	25.55831585966906	25.537535309324948
16-17	23.683666760589837	26.301657743965436	26.255165774396545	23.759509721048182
18-19	23.59056541748849	24.771884098807178	28.837231144923454	22.800319338780877
20-21	24.883329126333276	23.760823241425058	28.587680729787323	22.768166902454347
22-23	24.6805438151592	23.82560815253123	28.320418897341977	23.173429134967595
24-25	23.865022535770176	24.568157350042323	28.309247825377426	23.25757228881007
26-27	23.52437027079383	24.564927708085072	28.416387341430344	23.494314679690756
28-29	23.264652014652015	24.278787451864375	29.056189536958765	23.400370996524842
30-31	23.705698717820777	24.191989931353337	28.850030466578925	23.25228088424696
32-33	23.346599981215366	24.312599793369024	28.62015121630506	23.72064900911055
34-35	23.551352493660186	24.30590776744623	28.720531605146988	23.422208133746594
36-37	23.643328942394167	24.411978106354653	28.321440228047067	23.623252723204118
38-39	23.752582887198272	24.32199211045365	28.309617732694658	23.615807269653423
40-41	23.367846873998378	24.480858662765648	28.473025313973814	23.67826914926216
42-43	23.821499013806708	24.290997464074387	28.186930590776743	23.700572931342162
44-45	23.741546914623836	24.437987226448765	27.93627312858082	23.884192730346577
46-47	23.691532826148208	24.446909927679158	27.923593500516574	23.937963745656056
48-49	23.964731849347235	24.655654174884944	27.620221658683196	23.759392317084625
50-51	23.614453870700363	24.92574181868559	27.474705256657405	23.98509905395664
52-53	23.642763302334373	24.810478204259706	27.30440540581791	24.242353087588008
54-55	23.718183525875833	24.753803888419274	27.245585610970224	24.282426974734665
56-57	23.93491124260355	24.496454400300554	26.97661313045928	24.59202122663661
58-59	23.68413637644407	24.661524373062836	27.3113318305626	24.343007419930494
60-61	23.785629251501014	24.694690275876482	27.52953303637888	23.990147436243628
62-63	23.336937647709384	24.86871255534149	27.48612573773975	24.308224059209376
64-65	23.359514417206725	24.500211327134405	27.695242791396634	24.445031464262236
66-67	23.74251402098481	24.421227131148598	27.595599224663314	24.240659623203275
68-69	23.541725368648443	24.385625058701983	27.566333239410163	24.50631633323941
70-71	23.304686766225228	24.437987226448765	27.78482201559125	24.47250399173476
72-73	23.723584108199493	24.442331173100403	27.823095707711094	24.01098901098901
74-75	23.786395228702922	24.42413355874894	27.87827557058326	23.911195641964873
76-77	23.58428098121767	24.311717647500817	27.965337572409066	24.13866379887245
78-79	23.535268150652765	24.514769418615572	27.844932844932845	24.105029585798817
80-81	23.244928148774303	24.650370996524842	28.059782098243634	24.044918756457218
82-83	23.54430825584672	24.4865924673617	27.936625340471494	24.03247393632009
84-85	23.64002024046708	24.529004096229098	27.558147316318347	24.272828346985474
86-87	23.3576359537898	24.630881938574245	27.667300648069883	24.344181459566077
88-89	23.560627406781254	24.23405654174885	27.687963745656052	24.517352305813844
90-91	23.75786606555837	24.289823424438808	27.609303090072324	24.343007419930494
92-93	23.45578566732413	24.698741429510658	27.499765192072882	24.345707711092327
94-95	23.020451770451768	24.53214520522213	27.74619611158073	24.701206912745374
96-97	23.36092326476942	24.38186813186813	27.463604771297078	24.79360383206537
98-99	23.18592839750258	24.65489023877674	27.240483799389025	24.91869756433165
100-101	23.397201089508783	24.835751854982625	26.96874706490091	24.798299990607685
102-103	23.52763689302151	24.689349112426033	27.1535409035409	24.62947309101155
104-105	23.47444573328512	24.72304274933197	27.04506924518289	24.75744227220002
106-107	23.43235989565111	24.786852603673815	27.00802340570913	24.77276409496594
108-109	23.56654620935153	24.80845408416045	26.88957140207149	24.735428304416534
110-111	23.305978209824364	25.068916126608435	26.78301399455246	24.842091669014746
112-113	23.25537982522979	25.058380011904944	26.67693575674467	25.0093044061206
114-115	23.218640189445036	25.03266799804168	26.748545056008155	25.000146756505124
116-117	23.405149590787044	25.03683562330571	26.588804083793853	24.969210702113394
118-119	23.19206240372694	24.774932374046664	26.906910996731416	25.12609422549498
120-121	23.20657469914881	24.76172585852656	27.05570883475198	24.975990607572644
122-123	23.027015603536334	25.096568161271765	27.050356333579888	24.826059901612012
124-125	22.974697588574877	25.02544799627115	27.01444225154009	24.985412163613884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	85.5
2	166.5
3	247.0
4	219.5
5	214.5
6	312.5
7	558.0
8	897.5
9	1075.5
10	960.5
11	788.5
12	730.0
13	738.5
14	748.5
15	744.0
16	714.0
17	697.0
18	708.0
19	721.5
20	698.0
21	707.5
22	737.0
23	750.0
24	795.5
25	831.0
26	914.0
27	1062.5
28	1315.0
29	1791.0
30	2536.0
31	3228.5
32	4125.5
33	5062.0
34	5878.0
35	6676.5
36	7357.5
37	8395.5
38	9113.5
39	9142.5
40	9001.0
41	8897.5
42	9187.5
43	10120.5
44	11317.5
45	12578.0
46	14042.0
47	15265.0
48	16838.0
49	18298.0
50	19287.0
51	18803.0
52	17417.5
53	17309.5
54	17206.0
55	16613.0
56	15705.0
57	14421.0
58	13444.5
59	12242.5
60	10598.0
61	8910.0
62	7171.0
63	5659.0
64	4432.5
65	3532.5
66	2744.0
67	2079.0
68	1702.5
69	1452.5
70	1208.5
71	1056.0
72	946.0
73	878.0
74	711.0
75	604.5
76	507.5
77	367.0
78	268.0
79	154.5
80	100.0
81	91.5
82	81.0
83	64.5
84	42.0
85	19.5
86	13.0
87	6.5
88	1.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03522118906734291
2	0.002348079271156194
3	7.044237813468583E-4
4	0.006339814032121725
5	0.0025828871982718133
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	4.696158542312388E-4
14-15	2.348079271156194E-4
16-17	0.0
18-19	0.0
20-21	5.870198177890485E-4
22-23	0.0
24-25	3.5221189067342917E-4
26-27	5.870198177890485E-4
28-29	0.0
30-31	8.218277449046679E-4
32-33	0.0
34-35	0.0
36-37	7.044237813468583E-4
38-39	0.0
40-41	0.001995867380482765
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.348079271156194E-4
52-53	0.0012914435991359067
54-55	0.0
56-57	0.0
58-59	0.0
60-61	2.348079271156194E-4
62-63	3.5221189067342917E-4
64-65	0.0
66-67	1.174039635578097E-4
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	2.348079271156194E-4
78-79	0.0
80-81	0.0
82-83	0.0
84-85	1.174039635578097E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.348079271156194E-4
100-101	0.0
102-103	0.0
104-105	4.696158542312388E-4
106-107	2.348079271156194E-4
108-109	7.044237813468583E-4
110-111	0.0
112-113	0.0015262515262515263
114-115	0.0010566356720202875
116-117	3.5221189067342917E-4
118-119	0.0018784634169249552
120-121	0.001174039635578097
122-123	0.0035221189067342912
124-125	0.0027002911618296235
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	425880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.49495923034978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.93047841794417	59.87170613487076
2	9.996724622281338	14.094373893695131
3	2.434574908564856	5.14875776767536
4	0.8877137059490496	2.5031736603640384
5	0.4606087743204918	1.6235298383432222
6	0.27388828275624355	1.158464599594314
7	0.18233755959918133	0.8997715181074032
8	0.12102212811007104	0.68251599896717
9	0.08488126607840672	0.5385331252455977
>10	0.5656826632747026	7.6236267478733595
>50	0.042924809665788384	2.066313905825721
>100	0.017246575310105722	2.1443950665603304
>500	7.665144582269209E-4	0.32232119370120627
>1k	0.0011497716873403814	1.3225165491763662
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	0.47736451582605427	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1775	0.4167840706302245	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.25523621677467834	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	604	0.14182398797783413	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	589	0.13830186907109984	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	478	0.11223818916126609	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	2.3480792711561943E-4	0.0
20-21	0.0	0.0	0.0	9.392317084624777E-4	0.0
22-23	0.0	0.0	0.0	0.0028176951253874333	0.0
24-25	0.0	0.0	0.0	0.0073964497041420114	0.0
26-27	0.0	0.0	0.0	0.011975204282896591	0.0
28-29	0.0	0.0	0.0	0.02160232929463699	0.0
30-31	0.0	0.0	0.0	0.04015215553677092	0.0
32-33	0.0	0.0	0.0	0.06938574246266555	0.0
34-35	0.0	0.0	0.0	0.10261106414952569	0.0
36-37	0.0	0.0	0.0	0.15485582793275102	0.0
38-39	0.0	0.0	0.0	0.2214238752700291	0.0
40-41	0.0	0.0	0.0	0.29808866347327884	0.0
42-43	0.0	0.0	0.0	0.36817882971729127	0.0
44-45	0.0	0.0	0.0	0.43239879778341317	0.0
46-47	0.0	0.0	0.0	0.520099558561097	0.0
48-49	0.0	0.0	0.0	0.6062740678125293	0.0
50-51	0.0	0.0	0.0	0.689748285902132	0.0
52-53	0.0	0.0	0.0	0.7689959613036537	0.0
54-55	0.0	0.0	0.0	0.8475392129238284	0.0
56-57	0.0	0.0	0.0	0.9431060392598853	0.0
58-59	0.0	0.0	0.0	1.0473607588992204	0.0
60-61	0.0	0.0	0.0	1.1577204846435616	4.6961585423123885E-4
62-63	0.0	0.0	0.0	1.2551657743965436	4.6961585423123885E-4
64-65	0.0	0.0	0.0	1.351789236404621	4.6961585423123885E-4
66-67	0.0	0.0	0.0	1.4679017563632948	4.6961585423123885E-4
68-69	0.0	0.0	0.0	1.5969287123133276	4.6961585423123885E-4
70-71	0.0	0.0	0.0	1.7459143420681882	4.6961585423123885E-4
72-73	0.0	0.0	0.0	1.8803418803418803	5.870198177890485E-4
74-75	0.0	0.0	0.0	2.0043204658589273	7.044237813468582E-4
76-77	0.0	0.0	0.0	2.1298253029022263	7.044237813468582E-4
78-79	0.0	0.0	0.0	2.2886728655959425	7.044237813468582E-4
80-81	0.0	0.0	0.0	2.4591434206818823	7.044237813468582E-4
82-83	0.0	0.0	0.0	2.628557340095802	7.044237813468582E-4
84-85	0.0	0.0	0.0	2.787522306753076	7.044237813468582E-4
86-87	0.0	0.0	0.0	2.952709683478914	7.044237813468582E-4
88-89	0.0	0.0	0.0	3.1359772705926554	8.218277449046679E-4
90-91	0.0	0.0	0.0	3.3356814126044894	9.392317084624777E-4
92-93	0.0	0.0	0.0	3.542194984502677	9.392317084624777E-4
94-95	0.0	0.0	0.0	3.735794120409505	9.392317084624777E-4
96-97	0.0	0.0	0.0	3.935146050530666	0.0010566356720202873
98-99	0.0	0.0	0.0	4.164083779468395	0.001174039635578097
100-101	0.0	0.0	0.0	4.400652766037382	0.001174039635578097
102-103	0.0	0.0	0.0	4.648257725180802	0.001174039635578097
104-105	0.0	0.0	0.0	4.893514605053067	0.001174039635578097
106-107	0.0	0.0	0.0	5.156851695313234	0.001174039635578097
108-109	0.0	0.0	0.0	5.428054851131774	0.001174039635578097
110-111	0.0	0.0	0.0	5.717103409411102	0.001174039635578097
112-113	0.0	0.0	0.0	6.01894899971823	0.001174039635578097
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2485	0.0	32.102875	1
GTATCAA	3290	0.0	28.952738	1
TACAACG	85	0.007201745	28.002872	2
TACGGCA	85	0.007205875	27.999584	5
CATGGGT	520	0.0	25.175661	4
ATGGGTA	195	1.4613797E-6	24.409897	5
GAGTACT	1900	0.0	24.269377	12-13
CAACGCA	4055	0.0	24.210491	5
TATCAAC	4030	0.0	24.068226	2
ATCAACG	3980	0.0	23.922052	3
TCAACGC	4065	0.0	23.860998	4
CTAACGC	125	0.0016308697	23.802443	3
ATGGGAC	250	2.001434E-8	23.799648	5
AACGCAG	4305	0.0	23.357378	6
CATGGGG	1650	0.0	22.720512	4
GTACATA	160	2.59744E-4	22.325275	1
ACATGGG	3830	0.0	21.751577	3
ACGCAGA	4595	0.0	21.624271	7
GTACTTT	2170	0.0	21.249685	14-15
CGCAGAG	4720	0.0	21.177652	8
>>END_MODULE