FastQCFastQC Report
Sun 1 Jan 2017
ERR1142845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336894
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17670.5244973196316943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13950.4140768312881796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.2757543915890458No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5040.1496019519492778No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4700.13950975677809638No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.12407463475158358No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3610.10715536637636765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28000.033.1675031
GGTATCA20050.031.4729671
GTCTAGC953.271281E-431.3323211
CAACGCA33800.027.9877955
ATCAACG33000.027.949273
GTACTGT2803.6379788E-1227.6231736
TATCAAC33750.027.5044862
GTAAGTA1306.361527E-527.46382
AACGCAG35500.027.3179136
GTGTAGA1107.715963E-427.059731
TCAACGC35100.026.785684
ATGGGGG7500.026.178275
CTAACAC1608.71183E-626.0333923
ACGCAGA37500.025.3849877
CGCAGAG38050.025.0180578
CGCACTA1201.3007593E-824.7900358-59
TGTAAGT1451.3325454E-424.6336881
GCAGAGT39000.024.4086449
CTAGGCA2201.5307523E-724.3429134
GAGTACT16000.023.98435212-13
Produced by FastQC (version 0.11.5)