##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142845_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336894
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.077908778428824	33.0	14.0	33.0	14.0	33.0
2	30.147907056819058	33.0	27.0	33.0	27.0	33.0
3	29.80470711855955	33.0	27.0	33.0	27.0	33.0
4	31.103246124893882	33.0	33.0	33.0	27.0	33.0
5	31.846242438274352	33.0	33.0	33.0	27.0	33.0
6	34.48238911942629	37.0	33.0	37.0	27.0	37.0
7	35.694147120459256	37.0	37.0	37.0	33.0	37.0
8	35.54710086852244	37.0	37.0	37.0	33.0	37.0
9	35.92531478744056	37.0	37.0	37.0	33.0	37.0
10-11	35.93360819723711	37.0	37.0	37.0	33.0	37.0
12-13	35.999951023170496	37.0	37.0	37.0	33.0	37.0
14-15	36.0687293332621	37.0	37.0	37.0	37.0	37.0
16-17	36.0890858845809	37.0	37.0	37.0	37.0	37.0
18-19	36.10674277369143	37.0	37.0	37.0	37.0	37.0
20-21	36.0496135282908	37.0	37.0	37.0	37.0	37.0
22-23	36.109173805410606	37.0	37.0	37.0	37.0	37.0
24-25	36.14852446170012	37.0	37.0	37.0	37.0	37.0
26-27	36.07434682719194	37.0	37.0	37.0	37.0	37.0
28-29	36.07641572720203	37.0	37.0	37.0	37.0	37.0
30-31	36.02062221351523	37.0	37.0	37.0	37.0	37.0
32-33	35.94688685461897	37.0	37.0	37.0	37.0	37.0
34-35	35.875172903049624	37.0	37.0	37.0	35.0	37.0
36-37	35.798356456333444	37.0	37.0	37.0	33.0	37.0
38-39	35.74188023532625	37.0	37.0	37.0	33.0	37.0
40-41	35.66119610322535	37.0	37.0	37.0	33.0	37.0
42-43	35.565841184467516	37.0	37.0	37.0	33.0	37.0
44-45	35.45537765587989	37.0	37.0	37.0	33.0	37.0
46-47	35.301734373423095	37.0	37.0	37.0	33.0	37.0
48-49	35.0715788942516	37.0	37.0	37.0	30.0	37.0
50-51	35.01271468176934	37.0	37.0	37.0	30.0	37.0
52-53	34.98656253895884	37.0	37.0	37.0	30.0	37.0
54-55	34.917086680083344	37.0	37.0	37.0	27.0	37.0
56-57	34.88195396771685	37.0	37.0	37.0	27.0	37.0
58-59	34.84319993825952	37.0	37.0	37.0	27.0	37.0
60-61	34.81117354420085	37.0	37.0	37.0	27.0	37.0
62-63	34.79828076486966	37.0	37.0	37.0	27.0	37.0
64-65	34.77302504645378	37.0	37.0	37.0	27.0	37.0
66-67	34.75634027320166	37.0	37.0	37.0	27.0	37.0
68-69	34.678581393554055	37.0	37.0	37.0	27.0	37.0
70-71	34.674681056949666	37.0	37.0	37.0	27.0	37.0
72-73	34.66035013980658	37.0	37.0	37.0	27.0	37.0
74-75	34.614898157877555	37.0	37.0	37.0	27.0	37.0
76-77	34.56718285276675	37.0	37.0	37.0	27.0	37.0
78-79	34.46610951812736	37.0	37.0	37.0	27.0	37.0
80-81	34.38994906409731	37.0	37.0	37.0	27.0	37.0
82-83	34.406626416617684	37.0	37.0	37.0	27.0	37.0
84-85	34.371714842057145	37.0	37.0	37.0	27.0	37.0
86-87	34.29299275142923	37.0	37.0	37.0	27.0	37.0
88-89	34.22806134867347	37.0	37.0	37.0	27.0	37.0
90-91	34.176481326470636	37.0	37.0	37.0	27.0	37.0
92-93	34.094372413874986	37.0	37.0	37.0	24.5	37.0
94-95	34.042004309960994	37.0	37.0	37.0	22.0	37.0
96-97	33.98311189869811	37.0	37.0	37.0	22.0	37.0
98-99	33.89359115923703	37.0	37.0	37.0	22.0	37.0
100-101	33.83206735649789	37.0	37.0	37.0	22.0	37.0
102-103	33.74881119877469	37.0	37.0	37.0	22.0	37.0
104-105	33.60607193954181	37.0	33.0	37.0	22.0	37.0
106-107	33.53829839652828	37.0	33.0	37.0	22.0	37.0
108-109	33.448431850967964	37.0	33.0	37.0	22.0	37.0
110-111	33.41775454594027	37.0	33.0	37.0	22.0	37.0
112-113	33.360338860294334	37.0	33.0	37.0	14.0	37.0
114-115	33.23374117674996	37.0	33.0	37.0	14.0	37.0
116-117	33.197268280230574	37.0	33.0	37.0	14.0	37.0
118-119	33.023664713530074	37.0	33.0	37.0	14.0	37.0
120-121	32.93525411553782	37.0	33.0	37.0	14.0	37.0
122-123	32.84794623828266	37.0	33.0	37.0	14.0	37.0
124-125	31.25147672561696	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	6.0
16	4.0
17	5.0
18	42.0
19	255.0
20	1078.0
21	2510.0
22	3812.0
23	4911.0
24	4104.0
25	2929.0
26	2772.0
27	3045.0
28	3717.0
29	4704.0
30	6279.0
31	8468.0
32	11683.0
33	17432.0
34	28868.0
35	61420.0
36	168850.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.88925578605408	17.753698501594616	12.845723041471919	26.511322670879384
2	16.749879781291	20.750342845947888	38.18650819565075	24.313269177110357
3	19.75695619393637	25.88885524823832	28.725652579149525	25.62853597867579
4	13.857753502730944	17.670387081453338	35.92288055093802	32.548978864877704
5	15.065988280623847	34.57690914811891	33.188572717719765	17.168529853537482
6	29.07264599547632	35.34375797728662	18.876857409155402	16.70673861808165
7	25.389588416534576	32.06676283935006	23.589912554097133	18.953736190018226
8	25.666530125202584	31.50516186100079	21.78014449648851	21.048163517308115
9	26.65378427635992	15.7975505648661	21.62430912987468	35.9243560288993
10-11	25.03740048798732	25.15672585442305	27.08418671748384	22.72168694010579
12-13	26.380796048550113	24.21762064062644	27.092751618485618	22.308831692337833
14-15	24.33161270789242	24.322114143066194	26.225685900271007	25.12058724877038
16-17	23.04315303923489	26.373725860359638	26.930577570393062	23.65254353001241
18-19	22.912285336463896	25.046342538517365	29.71977791201077	22.32159421300797
20-21	24.212097960322538	24.05418361698054	29.404867739316664	22.328850683380256
22-23	23.896685604374078	24.17585353256514	29.203844532701677	22.723616330359103
24-25	23.168404117640073	24.77455683126937	29.37395367061254	22.683085380478015
26-27	22.965885108239757	24.6797925738592	29.3516300524502	23.002692265450843
28-29	22.704767671730576	24.639204022630263	29.793495877041444	22.86253242859772
30-31	23.2339828073425	24.321139369760868	29.783637291918975	22.661240530977654
32-33	22.85748633101213	24.54792902218502	29.425427582563064	23.16915706423979
34-35	23.04092681971184	24.290726459954765	29.66897599838525	22.99937072194815
36-37	23.158950825706047	24.636569123476708	29.112464324779197	23.09201572603805
38-39	23.07491377109714	24.52062072936888	29.269740630584103	23.134724868949878
40-41	22.604674219071182	24.4790221154945	29.567915984570654	23.348387680863663
42-43	23.23066967850326	24.415170395348078	29.12675537930441	23.227404546844248
44-45	23.159806942242962	24.614121949337182	28.78457318919304	23.44149791922682
46-47	23.171680113032586	24.53368121723747	28.616419407884976	23.678219261844973
48-49	23.459752919315868	24.573901583287324	28.592079407766242	23.374266089630567
50-51	23.020890844004345	24.92386329231153	28.129767820145208	23.925478043538916
52-53	22.99498653875266	24.939817686921366	27.87902984033506	24.18616593399091
54-55	23.15535450319685	24.93024512161095	27.687195378961928	24.227204996230267
56-57	23.35794047979483	24.800085486829683	27.515628061051846	24.32634597232364
58-59	23.171531698397715	24.520323900099143	28.171769161813508	24.136375239689638
60-61	23.180733405759675	24.912286950791643	28.14787440559939	23.759105237849294
62-63	22.932389412052807	24.86475656181126	27.964113181504484	24.238740844631447
64-65	22.688293647259968	24.739532315802595	28.106466722470568	24.465707314466865
66-67	23.217688649842383	24.49212511947378	28.12323757621091	24.166948654472918
68-69	23.03365450260319	24.452201582693668	28.15262367391524	24.361520240787904
70-71	22.899339258045558	24.456208777835165	28.5117870903014	24.132664873817877
72-73	23.21679816203316	24.349795484633148	28.780714408686414	23.652691944647277
74-75	23.218430723016738	24.472979631575512	28.698047457063648	23.61054218834411
76-77	22.90987669712135	24.53872731482306	28.683354408211486	23.868041579844103
78-79	22.898300355601464	24.632080120156488	28.71466989616912	23.754949628072925
80-81	22.59538608583115	24.786579755056486	28.880745872589003	23.73728828652336
82-83	23.04196572215593	24.51097377810231	28.895438921441162	23.551621578300594
84-85	22.98007681941501	24.77411292572738	28.4913058706893	23.754504384168314
86-87	22.633677061627694	24.856631462715274	28.640759408003703	23.86893206765333
88-89	23.010947063468034	24.29384316728704	28.674597944754137	24.02061182449079
90-91	23.18206913747351	24.296366216079836	28.508076724429642	24.013487922017017
92-93	22.98972377068158	24.630299144538046	28.347343674865087	24.032633409915285
94-95	22.181754498447585	24.61857438838329	28.739158310922726	24.460512802246402
96-97	22.569265110093976	24.536797924569743	28.187946357014372	24.70599060832191
98-99	22.440689537463733	24.76947394198446	27.95802815438159	24.83180836617022
100-101	22.575120290418617	24.894551088921407	27.72067095487291	24.80965766578706
102-103	22.921487057482555	24.754113688747335	27.735026054227816	24.58937319954229
104-105	22.682161223535697	25.15401797309231	27.638193192190386	24.52562761118161
106-107	22.589995324918185	24.99922081969768	27.690583791565558	24.720200063818577
108-109	22.675448881395578	25.137506567367083	27.439721217122347	24.747323334114988
110-111	22.49453090447115	25.365323708120975	27.40781197590926	24.732333411498605
112-113	22.567503187227935	25.43133323340007	27.158298740098815	24.84286483927318
114-115	22.48780393687582	25.389925242993666	27.34693514083911	24.775335679291405
116-117	22.59975719293663	25.36933091515704	27.224222527627468	24.806689364278867
118-119	22.338036655936143	25.063707832983813	27.45217645548466	25.146079055595383
120-121	22.292752573525046	25.219209717060657	27.780416275839144	24.707621433575152
122-123	22.1729476858778	25.638194296027166	27.716629859181687	24.47222815891335
124-125	22.178257791524494	25.366107777499085	27.66553325506807	24.79010117590835
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	53.5
2	114.5
3	189.5
4	178.0
5	174.5
6	254.0
7	419.5
8	664.0
9	839.5
10	812.5
11	711.5
12	700.5
13	717.0
14	729.0
15	730.0
16	738.5
17	740.5
18	723.5
19	733.5
20	743.5
21	740.5
22	731.5
23	735.0
24	791.5
25	852.5
26	908.0
27	1078.0
28	1321.5
29	1809.5
30	2554.5
31	3146.0
32	3968.0
33	4739.5
34	5492.5
35	6199.5
36	6512.0
37	7260.0
38	7623.0
39	7359.5
40	7061.5
41	6782.0
42	7163.5
43	7977.5
44	8915.5
45	9954.0
46	11230.0
47	12402.0
48	13598.0
49	14658.5
50	15147.5
51	14646.5
52	13541.0
53	13297.0
54	13209.5
55	12739.0
56	12008.5
57	10704.5
58	9614.0
59	8841.0
60	7717.0
61	6446.5
62	5198.0
63	4031.0
64	3017.0
65	2335.0
66	1791.5
67	1337.5
68	1113.5
69	939.0
70	798.5
71	711.0
72	638.5
73	618.5
74	501.0
75	406.0
76	337.0
77	225.5
78	156.0
79	88.5
80	58.5
81	45.5
82	30.0
83	22.0
84	16.5
85	8.5
86	3.5
87	1.0
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040368780684725755
2	0.0023746341579250446
3	0.0
4	0.004155609776368829
5	0.004749268315850089
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.001484146348703153
14-15	2.968292697406306E-4
16-17	0.0
18-19	1.484146348703153E-4
20-21	7.420731743515765E-4
22-23	0.0
24-25	5.936585394812612E-4
26-27	8.904878092218918E-4
28-29	0.0
30-31	0.0017809756184437836
32-33	0.0
34-35	0.0
36-37	1.484146348703153E-4
38-39	0.0
40-41	0.0010389024440922072
42-43	2.968292697406306E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	2.968292697406306E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	4.452439046109459E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	4.452439046109459E-4
100-101	2.968292697406306E-4
102-103	1.484146348703153E-4
104-105	4.452439046109459E-4
106-107	4.452439046109459E-4
108-109	2.968292697406306E-4
110-111	2.968292697406306E-4
112-113	7.420731743515765E-4
114-115	7.420731743515765E-4
116-117	2.968292697406306E-4
118-119	0.0013357317138328376
120-121	5.936585394812612E-4
122-123	0.0017809756184437836
124-125	0.0016325609835734682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	336894.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.62326577572915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.91486596052751	56.5730568319864
2	9.954213332282654	13.263644008499472
3	2.286910084500486	4.570842550946132
4	0.8296924209628338	2.2110727469566007
5	0.43406543475320164	1.4459428411809985
6	0.26419765312674304	1.0561026276952137
7	0.1875504489715229	0.8746656385730966
8	0.13203368801960502	0.703721239062388
9	0.09752450458191611	0.584766088956658
>10	0.795898266487777	10.66707495294408
>50	0.07367697804976596	3.3053457075240296
>100	0.02737995805903452	3.2197768541684892
>500	9.956348385103462E-4	0.47527196456772197
>1k	9.956348385103462E-4	1.0487159469386858
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1767	0.5244973196316943	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1395	0.4140768312881796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	929	0.2757543915890458	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	504	0.1496019519492778	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	470	0.13950975677809638	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	418	0.12407463475158358	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	361	0.10715536637636765	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.484146348703153E-4	0.0
12-13	0.0	0.0	0.0	2.968292697406306E-4	2.968292697406306E-4
14-15	0.0	0.0	0.0	2.968292697406306E-4	2.968292697406306E-4
16-17	0.0	0.0	0.0	4.4524390461094584E-4	2.968292697406306E-4
18-19	0.0	0.0	0.0	5.936585394812612E-4	2.968292697406306E-4
20-21	0.0	0.0	0.0	0.001484146348703153	2.968292697406306E-4
22-23	0.0	0.0	0.0	0.004155609776368828	2.968292697406306E-4
24-25	0.0	0.0	0.0	0.00949853663170018	2.968292697406306E-4
26-27	0.0	0.0	0.0	0.017512926914697205	2.968292697406306E-4
28-29	0.0	0.0	0.0	0.03428378065504283	2.968292697406306E-4
30-31	0.0	0.0	0.0	0.06085000029682927	2.968292697406306E-4
32-33	0.0	0.0	0.0	0.09394646387290959	2.968292697406306E-4
34-35	0.0	0.0	0.0	0.1357993909063385	2.968292697406306E-4
36-37	0.0	0.0	0.0	0.19368109850576146	2.968292697406306E-4
38-39	0.0	0.0	0.0	0.26848207448040035	2.968292697406306E-4
40-41	0.0	0.0	0.0	0.3530784163564801	2.968292697406306E-4
42-43	0.0	0.0	0.0	0.43144134356800656	2.968292697406306E-4
44-45	0.0	0.0	0.0	0.504609758559072	2.968292697406306E-4
46-47	0.0	0.0	0.0	0.5929164663069095	2.968292697406306E-4
48-49	0.0	0.0	0.0	0.6841914667521536	2.968292697406306E-4
50-51	0.0	0.0	0.0	0.7762085403717489	2.968292697406306E-4
52-53	0.0	0.0	0.0	0.8636247603103646	2.968292697406306E-4
54-55	0.0	0.0	0.0	0.9504473217094991	2.968292697406306E-4
56-57	0.0	0.0	0.0	1.0484009807239072	2.968292697406306E-4
58-59	0.0	0.0	0.0	1.1531817129423496	2.968292697406306E-4
60-61	0.0	0.0	0.0	1.2544004939239048	2.968292697406306E-4
62-63	0.0	0.0	0.0	1.3496826895106473	2.968292697406306E-4
64-65	0.0	0.0	0.0	1.4523856168409055	2.968292697406306E-4
66-67	0.0	0.0	0.0	1.5589473246777918	2.968292697406306E-4
68-69	0.0	0.0	0.0	1.6839124472385973	2.968292697406306E-4
70-71	0.0	0.0	0.0	1.8217896430331202	2.968292697406306E-4
72-73	0.0	0.0	0.0	1.9494262290215914	2.968292697406306E-4
74-75	0.0	0.0	0.0	2.0672674491086216	2.968292697406306E-4
76-77	0.0	0.0	0.0	2.2095970839492542	2.968292697406306E-4
78-79	0.0	0.0	0.0	2.36765867008614	2.968292697406306E-4
80-81	0.0	0.0	0.0	2.548872939262795	2.968292697406306E-4
82-83	0.0	0.0	0.0	2.719401354728787	5.936585394812612E-4
84-85	0.0	0.0	0.0	2.8880003799414653	5.936585394812612E-4
86-87	0.0	0.0	0.0	3.058677210042328	5.936585394812612E-4
88-89	0.0	0.0	0.0	3.2471637963276283	5.936585394812612E-4
90-91	0.0	0.0	0.0	3.435798797247799	5.936585394812612E-4
92-93	0.0	0.0	0.0	3.6538198958722923	5.936585394812612E-4
94-95	0.0	0.0	0.0	3.85521855539131	5.936585394812612E-4
96-97	0.0	0.0	0.0	4.058695019798512	5.936585394812612E-4
98-99	0.0	0.0	0.0	4.265881850077472	5.936585394812612E-4
100-101	0.0	0.0	0.0	4.517296241547786	5.936585394812612E-4
102-103	0.0	0.0	0.0	4.7862235599327985	5.936585394812612E-4
104-105	0.0	0.0	0.0	5.0419419758143516	8.904878092218918E-4
106-107	0.0	0.0	0.0	5.298847708774867	8.904878092218918E-4
108-109	0.0	0.0	0.0	5.567923441794749	8.904878092218918E-4
110-111	0.0	0.0	0.0	5.840264296781777	8.904878092218918E-4
112-113	0.0	0.0	0.0	6.1629177129898425	8.904878092218918E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2800	0.0	33.167503	1
GGTATCA	2005	0.0	31.472967	1
GTCTAGC	95	3.271281E-4	31.332321	1
CAACGCA	3380	0.0	27.987795	5
ATCAACG	3300	0.0	27.94927	3
GTACTGT	280	3.6379788E-12	27.623173	6
TATCAAC	3375	0.0	27.504486	2
GTAAGTA	130	6.361527E-5	27.4638	2
AACGCAG	3550	0.0	27.317913	6
GTGTAGA	110	7.715963E-4	27.05973	1
TCAACGC	3510	0.0	26.78568	4
ATGGGGG	750	0.0	26.17827	5
CTAACAC	160	8.71183E-6	26.033392	3
ACGCAGA	3750	0.0	25.384987	7
CGCAGAG	3805	0.0	25.018057	8
CGCACTA	120	1.3007593E-8	24.79003	58-59
TGTAAGT	145	1.3325454E-4	24.633688	1
GCAGAGT	3900	0.0	24.408644	9
CTAGGCA	220	1.5307523E-7	24.342913	4
GAGTACT	1600	0.0	23.984352	12-13
>>END_MODULE