FastQCFastQC Report
Sun 1 Jan 2017
ERR1142844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98021
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5820.5937503188092348No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4290.43766131747278647No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3490.35604615337529716No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2530.25810795645831No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2390.24382530274124933No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1890.1928158251803185No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1680.17139184460472756No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1620.16527070729741586No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1610.16425051774619726No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1600.16323032819497862No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1530.1560890013364483No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1480.15098805358035522No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1440.14690729537548075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.12854388345354567No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1200.12242274614623397No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA1180.12038236704379675No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1140.11630160883892228No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1100.1122208506340478No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1050.10711990287795473No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1000.10201895512186164No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA990.10099876557064302No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAA304.2273714E-579.39881
CACGGGT150.00422605559.518658108-109
TCGAACG150.00422605559.51865852-53
AACCTCG300.00422830159.4882479
TTCGATG150.0042346259.48824774-75
CTAGATA350.00778115350.989933
TACATGA558.498383E-443.264182
ATATAAA600.001297378339.69941
GTACAAA700.00276537534.028061
GATCTGC800.00534370929.7441236
ATCGGGC400.005356639629.74412322-23
CTACCAT450.00949548826.45273694-95
GGTTCTA450.00949548826.45273696-97
GGATCTG900.00949637426.4392195
TGGGGGA2251.8784704E-723.79536
GTATAAA3204.802132E-1022.3309141
ATGGGGG4000.022.3080925
TATAAAA2403.5011908E-722.3080922
GAGTACT953.2926167E-521.91672312-13
CATGGGG4350.021.8807354
Produced by FastQC (version 0.11.5)