ERR1142843_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142843_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	107488
Sequences flagged as poor quality	0
Sequence length	125
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1136	1.0568621613575468	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1128	1.0494194700803812	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	706	0.6568175052098839	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	503	0.46795921405180113	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	402	0.3739952366775826	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.32840875260494196	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.3209660613277761	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	332	0.30887168800238163	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	326	0.30328966954450726	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	282	0.2623548675200953	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	230	0.21397737421851742	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	226	0.2102560285799345	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	204	0.1897886275677285	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	194	0.1804852634712712	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	190	0.1767639178326883	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	189	0.17583358142304256	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	182	0.16932122655552248	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	173	0.16094819886871092	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	172	0.1600178624590652	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	172	0.1600178624590652	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	169	0.15722685323012803	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	167	0.15536618041083655	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	158	0.146993152724025	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	150	0.13955046144685918	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	143	0.1330381065793391	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	143	0.1330381065793391	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	142	0.13210777016969336	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	140	0.1302470973504019	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	139	0.12931676094075617	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.12745608812146472	No Hit
GGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTG	133	0.12373474248288181	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	133	0.12373474248288181	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	133	0.12373474248288181	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	132	0.12280440607323607	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	131	0.12187406966359035	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	128	0.11908306043465318	No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGA	127	0.11815272402500746	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG	127	0.11815272402500746	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	125	0.116292051205716	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	124	0.11536171479607026	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	123	0.11443137838642453	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	122	0.11350104197677881	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	122	0.11350104197677881	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	122	0.11350104197677881	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	116	0.10791902351890442	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	116	0.10791902351890442	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	114	0.10605835069961299	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	113	0.10512801428996725	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	111	0.10326734147067579	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	111	0.10326734147067579	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	110	0.10233700506103006	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	109	0.10140666865138435	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCAACG	25	1.1962038E-7	119.05493	1
GCGCAGA	80	0.0	104.173065	1
GCAACGC	25	0.002048006	71.43296	1
TGTTATC	40	1.764759E-4	59.499767	2
GTTATCA	45	3.1580488E-4	52.888683	3
CGGATTA	30	0.001306166	39.66651	38-39
GGCCTTA	30	0.001306166	39.66651	38-39
AGAGCAC	30	0.001306166	39.66651	42-43
TCTCTGC	95	7.4094987E-6	37.578804	8
TTCTCTG	100	1.0532896E-5	35.69986	7
GTGTTAT	85	1.6955935E-4	35.016155	1
GGGCTGG	35	0.002784022	33.999866	16-17
TATTCTC	105	1.4712872E-5	33.999866	5
TCTGGAT	35	0.002784022	33.999866	110-111
AGTGGGT	40	0.0053527276	29.749884	34-35
TACGGGC	120	3.665361E-5	29.749884	4
TTATCAA	100	4.4122996E-4	29.749884	4
AGAACCT	40	0.0053527276	29.749884	22-23
GTTGTTT	40	0.0053527276	29.749884	112-113
CGGGCGT	120	3.665361E-5	29.749884	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content