FastQCFastQC Report
Sun 1 Jan 2017
ERR1142841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106672
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4720.4424778761061947No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3900.3656067196640168No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2300.21561421928903554No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1930.18092845357732112No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1830.1715539223038848No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1790.16780410979451027No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1770.16592920353982302No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1730.16217939103044848No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1580.148117594120294No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1500.14061796910154492No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1390.13030598470076496No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1340.1256187190640468No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1260.11811909404529773No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1190.11155692215389232No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC1190.11155692215389232No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1160.10874456277186141No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1160.10874456277186141No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA1150.10780710964451777No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1150.10780710964451777No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1130.1059322033898305No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1130.1059322033898305No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1110.10405729713514325No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1110.10405729713514325No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1070.1003074846257687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA358.891475E-785.017461
TACAAAA401.972534E-674.3553162
TGGGATG300.00423043959.4842576
TAGAACG350.00778507850.9865072
GAAAACT350.00778507850.9865079
TTAGTAT400.005347131829.75611116-117
GGGTCCG400.00535943829.74212644-45
TATTCTC800.0053475429.7421265
GGGTGAG850.00717424828.005751
GTAAAAA850.00717424828.005751
TGGGCAA656.365719E-527.45427318-19
AGTTAGC450.00948055726.46231896-97
GATGTCT450.00952413126.43744924-25
AACAGTA900.0095031426.4374495
TGGGGGA2109.66229E-825.4932546
GAGTACT600.001286399924.78510712-13
TAAGGTG1200.001282175124.7851074
GTATAAG1451.3270881E-424.6257481
ATGGGCA902.170713E-523.13276718-19
GAAAAAA7800.022.8893171
Produced by FastQC (version 0.11.5)