FastQCFastQC Report
Sun 1 Jan 2017
ERR1142840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347221
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4520.13017645822113294No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3810.10972838624391956No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3550.10224035988606679No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT900.00951157226.4492234
GTACTAG1401.0484211E-425.5193371
CATGGGG15400.025.1181744
TTAGAGT1200.001284694824.7961464
GGTATCA20700.023.8755781
GTAAAAA2853.1523086E-922.9823271
GTATCCA1300.002040272622.9019681
TAGAGTG1300.002047134822.888755
ATGGGGA4200.022.6707635
ATGGGCG802.6181014E-422.30204818-19
ATGGGGG9350.021.0037945
TAGCCTG1450.003857968420.5209485
TAGGCAT2701.0989006E-619.8369185
ATACGGG1801.709238E-519.8369163
ATGGGGC4555.456968E-1219.6189295
GTATCAA24650.019.5552582
AGAGAGT1550.00569809919.1831766
GATTACT1401.5681235E-619.12017834-35
GTATAAG2501.2492968E-519.0544381
CGAATAG1101.0210062E-418.9311438-39
Produced by FastQC (version 0.11.5)