FastQCFastQC Report
Sun 1 Jan 2017
ERR1142840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347221
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14360.41356945576448434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.4057934283928679No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4840.13939249066156714No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.1270084470697337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.1267204460559701No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4100.11808041564306307No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA2600.052.6720961
GTCAACG1101.7644197E-1048.716481
GCGTAAG650.001930279236.641461
GGTATCA18750.035.2490841
GTATCAA26150.032.105061
ATCAACG29500.029.0480063
CAACGCA29850.028.5039465
GTCCTAA1055.872768E-428.3535081
TATCAAC29850.028.3086952
GTAGCAC850.00719878228.0037963
AACGCAG31800.027.1302746
TCAACGC31750.026.9855984
TCTAACG900.00951368926.4480292
ACGCAGA33000.025.9634157
ATGGGTA1200.001286116924.7914565
CGCAGAG34950.024.5148128
GTAGGAC1751.7443588E-523.8032263
TTTAGGG2802.586603E-923.378172
GGCTATT2552.466004E-823.3331367
GCAGAGT37200.023.0320639
Produced by FastQC (version 0.11.5)