##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142840_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347221
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.539008297309206	27.0	14.0	33.0	14.0	33.0
2	24.322990256925703	27.0	14.0	27.0	14.0	33.0
3	24.106782135873118	27.0	14.0	27.0	14.0	33.0
4	29.77191759714994	33.0	27.0	33.0	27.0	33.0
5	31.576989871004347	33.0	33.0	33.0	27.0	33.0
6	35.55981349054348	37.0	37.0	37.0	33.0	37.0
7	35.598552506904824	37.0	37.0	37.0	33.0	37.0
8	35.96025298009049	37.0	37.0	37.0	33.0	37.0
9	36.144086907185915	37.0	37.0	37.0	37.0	37.0
10-11	36.05153490140285	37.0	37.0	37.0	35.0	37.0
12-13	36.14961796665524	37.0	37.0	37.0	37.0	37.0
14-15	36.133626710366016	37.0	37.0	37.0	37.0	37.0
16-17	36.138635047995365	37.0	37.0	37.0	37.0	37.0
18-19	36.1210482660899	37.0	37.0	37.0	37.0	37.0
20-21	36.06807623962836	37.0	37.0	37.0	37.0	37.0
22-23	36.11074934983772	37.0	37.0	37.0	37.0	37.0
24-25	36.132237105474616	37.0	37.0	37.0	37.0	37.0
26-27	36.05929652872378	37.0	37.0	37.0	37.0	37.0
28-29	36.04996097586263	37.0	37.0	37.0	37.0	37.0
30-31	36.026880574619625	37.0	37.0	37.0	37.0	37.0
32-33	35.97279686424496	37.0	37.0	37.0	37.0	37.0
34-35	35.90753007450586	37.0	37.0	37.0	35.0	37.0
36-37	35.84275288649016	37.0	37.0	37.0	33.0	37.0
38-39	35.78765973256226	37.0	37.0	37.0	33.0	37.0
40-41	35.71180026553694	37.0	37.0	37.0	33.0	37.0
42-43	35.63956241125969	37.0	37.0	37.0	33.0	37.0
44-45	35.551619861701916	37.0	37.0	37.0	33.0	37.0
46-47	35.41105232690419	37.0	37.0	37.0	33.0	37.0
48-49	35.20757241065488	37.0	37.0	37.0	33.0	37.0
50-51	35.14136241759571	37.0	37.0	37.0	33.0	37.0
52-53	35.10999622718672	37.0	37.0	37.0	33.0	37.0
54-55	35.03223451346548	37.0	37.0	37.0	33.0	37.0
56-57	35.00007200025344	37.0	37.0	37.0	33.0	37.0
58-59	34.95599344509693	37.0	37.0	37.0	27.0	37.0
60-61	34.91254849217069	37.0	37.0	37.0	27.0	37.0
62-63	34.896011473960385	37.0	37.0	37.0	27.0	37.0
64-65	34.8749341197681	37.0	37.0	37.0	27.0	37.0
66-67	34.846470979577845	37.0	37.0	37.0	27.0	37.0
68-69	34.773429602472206	37.0	37.0	37.0	27.0	37.0
70-71	34.75895323151538	37.0	37.0	37.0	27.0	37.0
72-73	34.73358322221294	37.0	37.0	37.0	27.0	37.0
74-75	34.69279219862854	37.0	37.0	37.0	27.0	37.0
76-77	34.646028034018684	37.0	37.0	37.0	27.0	37.0
78-79	34.55225490393726	37.0	37.0	37.0	27.0	37.0
80-81	34.45890513534607	37.0	37.0	37.0	27.0	37.0
82-83	34.46683524326006	37.0	37.0	37.0	27.0	37.0
84-85	34.432522802480264	37.0	37.0	37.0	27.0	37.0
86-87	34.35010411236648	37.0	37.0	37.0	27.0	37.0
88-89	34.29227350880275	37.0	37.0	37.0	27.0	37.0
90-91	34.2187569300244	37.0	37.0	37.0	27.0	37.0
92-93	34.12365899527966	37.0	37.0	37.0	27.0	37.0
94-95	34.07500122400431	37.0	37.0	37.0	27.0	37.0
96-97	34.020522952240796	37.0	37.0	37.0	24.5	37.0
98-99	33.92093076167628	37.0	37.0	37.0	22.0	37.0
100-101	33.85054331391247	37.0	37.0	37.0	22.0	37.0
102-103	33.76002891530178	37.0	33.0	37.0	22.0	37.0
104-105	33.60986086671025	37.0	33.0	37.0	22.0	37.0
106-107	33.54088174390374	37.0	33.0	37.0	22.0	37.0
108-109	33.46329715080597	37.0	33.0	37.0	22.0	37.0
110-111	33.41850579314039	37.0	33.0	37.0	22.0	37.0
112-113	33.35240236045631	37.0	33.0	37.0	22.0	37.0
114-115	33.19901302052583	37.0	33.0	37.0	14.0	37.0
116-117	33.14995924785656	37.0	33.0	37.0	14.0	37.0
118-119	32.9877297168086	37.0	33.0	37.0	14.0	37.0
120-121	32.858453261755486	37.0	33.0	37.0	14.0	37.0
122-123	32.78880597659703	37.0	33.0	37.0	14.0	37.0
124-125	31.097681303838193	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	213.0
16	99.0
17	21.0
18	37.0
19	228.0
20	908.0
21	2074.0
22	3370.0
23	4179.0
24	3359.0
25	2606.0
26	2618.0
27	3198.0
28	4013.0
29	5269.0
30	6895.0
31	9528.0
32	13607.0
33	20749.0
34	35091.0
35	79411.0
36	149743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.9865397469288	11.391661096641851	10.962096941480166	20.659702214949178
2	18.383422375185404	19.75807496795933	34.47806114367179	27.380441513183474
3	22.74478857662909	24.65137176068061	27.546958971021084	25.05688069166921
4	15.50542769174052	17.45645893877034	34.76718097010089	32.27093239938825
5	16.27215100556133	34.8506274138224	31.45939594319435	17.417825637421917
6	30.120010022435277	33.82283905639348	18.517025179928634	17.54012574124261
7	26.447997096949784	31.670895481552098	22.725871995069422	19.155235426428703
8	26.24207637210883	31.14270162230971	21.11306631799344	21.502155687588022
9	26.99030300586658	15.921848044905119	21.052010103075563	36.03583884615274
10-11	25.96890741055409	24.56432646642916	26.16604410447525	23.300722018541506
12-13	27.072605264673694	23.717095789413055	26.102471055814757	23.1078278900985
14-15	25.251784886282802	23.693843402328778	25.019224067668716	26.035147643719707
16-17	24.050388657368075	25.82749891279617	25.926859262544603	24.19525316729115
18-19	23.79352601588904	24.614474087791475	28.339772565271925	23.25222733104756
20-21	24.99719197394152	23.745244355867623	28.147077204876464	23.1104864653144
22-23	25.125496441747476	23.369554260831	27.93681833760055	23.56813095982098
24-25	24.20799493116756	24.16407465007776	28.041731467081387	23.58619895167329
26-27	23.91362261156815	24.317400614307047	27.958746675383512	23.81023009874129
28-29	23.61334711898186	24.2169972438303	28.55126850046512	23.61838713672272
30-31	24.06175929312624	23.88852568551836	28.544952897162883	23.504762124192514
32-33	23.724371509787716	24.233701302628585	28.00493057735563	24.036996610228066
34-35	23.910852166199625	24.16069304563952	28.185075211464746	23.74337957669611
36-37	24.032135199390588	24.274344400748227	27.76981773829598	23.9237026615652
38-39	23.95304431471599	24.16227705121522	28.011698601179074	23.872980032889714
40-41	23.67853002800838	24.086055570355757	28.23604801025294	23.999366391382924
42-43	24.085380780540348	24.039876620365703	27.828673956932327	24.046068642161618
44-45	24.081636767361424	24.164005057297803	27.582433090164475	24.171925085176298
46-47	23.985876430285035	24.115044884957996	27.413808496605906	24.485270188151063
48-49	24.33104564528067	24.43659801682502	27.17995167342989	24.05240466446442
50-51	23.984470962975976	24.633770183500108	27.01323798566041	24.368520867863506
52-53	23.91423305109153	24.65655780196993	26.741115143136916	24.688094003801623
54-55	23.972628383651912	24.641222737104034	26.708782014912693	24.67736686433136
56-57	24.16299705374963	24.442502037607174	26.365052804985872	25.029448103657327
58-59	23.887524084084777	24.516950299665055	26.98526298812572	24.610262628124453
60-61	24.11648489002681	24.478790165341383	27.078431316078234	24.32629362855357
62-63	23.770053899467342	24.585529944228522	26.977813809956498	24.666602346347638
64-65	23.565970952217754	24.356965736519392	27.263615967928207	24.813447343334648
66-67	24.058452685753455	24.226501277284495	27.02111911433928	24.693926922622765
68-69	23.879604056206276	24.138948969100372	27.192479717528606	24.788967257164746
70-71	23.77001967046924	24.136212959469617	27.30984013063726	24.783927239423882
72-73	23.86434000247681	24.152053015226613	27.681649439406026	24.301957542890552
74-75	23.961828345635777	24.07242073492099	27.64204930001354	24.3237016194297
76-77	23.698739419562756	24.16458105932533	27.6731534095	24.463526111611912
78-79	23.6327871874109	24.40837391747619	27.45744065019109	24.50139824492182
80-81	23.3663862496796	24.334069655925187	27.915506262582046	24.384037831813167
82-83	23.64488322998897	24.19194115563287	27.866114088721595	24.29706152565657
84-85	23.70766745098943	24.340981680255513	27.481200733826583	24.470150134928474
86-87	23.282865955688166	24.511334279896666	27.52195287727413	24.68384688714104
88-89	23.75317161116407	23.940804271631038	27.62923325490106	24.676790862303836
90-91	23.800403777421298	23.954196318771043	27.601153156059112	24.644246747748554
92-93	23.80472379262775	24.3268696305811	27.28953605916693	24.57887051762422
94-95	23.04209710818182	24.194101163236095	27.809089887996407	24.954711840585677
96-97	23.421250442801558	24.05874068676722	27.401856454534716	25.118152415896507
98-99	23.215885166912912	24.385038750982815	27.161112503132046	25.237963578972227
100-101	23.570748678425833	24.439221988459717	26.833679657045924	25.15634967606853
102-103	23.553078739703935	24.440844421404297	26.99513276884972	25.010944070042047
104-105	23.573764495638887	24.55384464735979	26.844346394828587	25.028044462172737
106-107	23.481433907706663	24.5055714117027	26.7463762063712	25.26661847421944
108-109	23.511716618114427	24.708218552764023	26.47913933653111	25.300925492590444
110-111	23.228899215340107	24.970011851258782	26.604419957922993	25.19666897547812
112-113	23.327928483176326	24.944919488852857	26.262387714957995	25.46476431301282
114-115	23.410364670034387	24.923967075439638	26.318192029215076	25.3474762253109
116-117	23.486676881249714	24.785580157596424	26.26103059766831	25.466712363485556
118-119	23.101503245102826	24.64910120659936	26.567074338559195	25.682321209738625
120-121	23.158040254191526	24.6527762777617	26.788895176956295	25.40028829109048
122-123	23.045308380099048	25.118408338481522	26.672225270333904	25.164058011085526
124-125	23.106842813159723	24.7302072802993	26.672686061213497	25.490263845327483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26.0
1	28.5
2	87.5
3	150.5
4	162.5
5	170.0
6	177.0
7	276.5
8	514.5
9	739.5
10	743.0
11	669.5
12	686.5
13	666.0
14	624.5
15	604.0
16	608.0
17	590.5
18	571.5
19	589.5
20	586.0
21	574.5
22	560.5
23	568.0
24	585.0
25	593.5
26	638.5
27	721.0
28	827.0
29	1091.5
30	1560.0
31	1972.0
32	2481.0
33	3086.5
34	3699.0
35	4285.5
36	4873.0
37	5745.0
38	6529.5
39	6849.0
40	6776.5
41	6826.5
42	7279.0
43	8142.0
44	9354.0
45	10574.5
46	11983.0
47	13347.5
48	14579.0
49	15565.5
50	16266.5
51	16109.0
52	15481.5
53	15258.5
54	14910.5
55	14474.0
56	13858.5
57	12473.0
58	11097.0
59	10020.0
60	8684.0
61	7315.0
62	5988.5
63	4839.5
64	3720.5
65	2817.0
66	2225.5
67	1711.0
68	1405.5
69	1243.0
70	1064.5
71	951.0
72	839.5
73	760.5
74	619.0
75	513.0
76	422.5
77	320.5
78	244.5
79	171.5
80	125.0
81	99.0
82	86.5
83	67.0
84	46.5
85	26.5
86	21.0
87	10.5
88	2.5
89	2.5
90	1.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03600012672044606
2	0.0017280060825814105
3	8.640030412907052E-4
4	0.005760020275271369
5	5.760020275271369E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	2.8800101376356843E-4
14-15	0.0
16-17	0.0
18-19	1.4400050688178422E-4
20-21	5.760020275271369E-4
22-23	0.0
24-25	2.8800101376356843E-4
26-27	1.4400050688178422E-4
28-29	0.0
30-31	5.760020275271369E-4
32-33	0.0
34-35	0.0
36-37	1.4400050688178422E-4
38-39	0.0
40-41	0.0010080035481724895
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.4400050688178422E-4
52-53	2.8800101376356843E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.4400050688178422E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0011520040550542737
100-101	7.200025344089212E-4
102-103	2.8800101376356843E-4
104-105	0.001296004561936058
106-107	5.760020275271369E-4
108-109	1.4400050688178422E-4
110-111	1.4400050688178422E-4
112-113	5.760020275271369E-4
114-115	8.640030412907052E-4
116-117	0.0014400050688178423
118-119	0.0015840055756996263
120-121	7.200025344089212E-4
122-123	0.003312011658281037
124-125	0.0028800101376356847
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	347221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.6330131037179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.52944567718656	62.69997929155069
2	8.361998083731898	11.979902366104621
3	1.8363501509432407	3.9462988327659447
4	0.6915217087442609	1.9814313449593213
5	0.33273836808548085	1.191752594058848
6	0.1924752499343442	0.8272549260412954
7	0.1484393076338374	0.7443208408189039
8	0.09875234305414873	0.5659142307216546
9	0.08204514624656643	0.528942693255907
>10	0.6480807304761605	9.356722329648662
>50	0.059608398785685496	2.9079799384258136
>100	0.017661747788341838	2.369652566747583
>500	0.0	0.0
>1k	8.830873894170921E-4	0.8998480449008108
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1436	0.41356945576448434	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1409	0.4057934283928679	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	484	0.13939249066156714	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.1270084470697337	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.1267204460559701	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	410	0.11808041564306307	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.8800101376356843E-4	0.0
14-15	0.0	0.0	0.0	2.8800101376356843E-4	0.0
16-17	0.0	0.0	0.0	5.760020275271369E-4	0.0
18-19	0.0	0.0	0.0	5.760020275271369E-4	0.0
20-21	0.0	0.0	0.0	0.0010080035481724895	0.0
22-23	0.0	0.0	0.0	0.0027360096307539002	0.0
24-25	0.0	0.0	0.0	0.00748802635785278	0.0
26-27	0.0	0.0	0.0	0.01267204460559701	0.0
28-29	0.0	0.0	0.0	0.02390408414237618	0.0
30-31	0.0	0.0	0.0	0.04046414243378137	0.0
32-33	0.0	0.0	0.0	0.07516826459229137	0.0
34-35	0.0	0.0	0.0	0.11448040297101847	0.0
36-37	0.0	0.0	0.0	0.1723686067374957	0.0
38-39	0.0	0.0	0.0	0.24926487741236847	0.0
40-41	0.0	0.0	0.0	0.32904115822487695	0.0
42-43	0.0	0.0	0.0	0.40233741622770514	0.0
44-45	0.0	0.0	0.0	0.47620967625806043	0.0
46-47	0.0	0.0	0.0	0.569234003703693	0.0
48-49	0.0	0.0	0.0	0.6675863499039516	0.0
50-51	0.0	0.0	0.0	0.7596026738014118	0.0
52-53	0.0	0.0	0.0	0.8405309586689744	0.0
54-55	0.0	0.0	0.0	0.9306752759769714	0.0
56-57	0.0	0.0	0.0	1.0334916378905654	0.0
58-59	0.0	0.0	0.0	1.1452360312308298	0.0
60-61	0.0	0.0	0.0	1.2607244377500209	0.0
62-63	0.0	0.0	0.0	1.369300819938886	0.0
64-65	0.0	0.0	0.0	1.4709651777974257	0.0
66-67	0.0	0.0	0.0	1.585013579247799	0.0
68-69	0.0	0.0	0.0	1.710438020741833	0.0
70-71	0.0	0.0	0.0	1.8482465058277004	0.0
72-73	0.0	0.0	0.0	1.981446974693351	2.8800101376356843E-4
74-75	0.0	0.0	0.0	2.1097514263250208	2.8800101376356843E-4
76-77	0.0	0.0	0.0	2.247559911410888	2.8800101376356843E-4
78-79	0.0	0.0	0.0	2.3994804461711707	2.8800101376356843E-4
80-81	0.0	0.0	0.0	2.5666650346609217	2.8800101376356843E-4
82-83	0.0	0.0	0.0	2.7388896408915357	2.8800101376356843E-4
84-85	0.0	0.0	0.0	2.8971461979546165	2.8800101376356843E-4
86-87	0.0	0.0	0.0	3.067210796582004	2.8800101376356843E-4
88-89	0.0	0.0	0.0	3.245195423087889	2.8800101376356843E-4
90-91	0.0	0.0	0.0	3.4499641438737862	2.8800101376356843E-4
92-93	0.0	0.0	0.0	3.6423488210678503	2.8800101376356843E-4
94-95	0.0	0.0	0.0	3.8445255327298753	5.760020275271369E-4
96-97	0.0	0.0	0.0	4.054910273284162	5.760020275271369E-4
98-99	0.0	0.0	0.0	4.279119062499101	5.760020275271369E-4
100-101	0.0	0.0	0.0	4.52607993180136	5.760020275271369E-4
102-103	0.0	0.0	0.0	4.772608799582975	5.760020275271369E-4
104-105	0.0	0.0	0.0	5.016257657226953	5.760020275271369E-4
106-107	0.0	0.0	0.0	5.243490457086409	5.760020275271369E-4
108-109	0.0	0.0	0.0	5.489587323347378	5.760020275271369E-4
110-111	0.0	0.0	0.0	5.760452276792014	5.760020275271369E-4
112-113	0.0	0.0	0.0	6.03938125862203	5.760020275271369E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	260	0.0	52.672096	1
GTCAACG	110	1.7644197E-10	48.71648	1
GCGTAAG	65	0.0019302792	36.64146	1
GGTATCA	1875	0.0	35.249084	1
GTATCAA	2615	0.0	32.10506	1
ATCAACG	2950	0.0	29.048006	3
CAACGCA	2985	0.0	28.503946	5
GTCCTAA	105	5.872768E-4	28.353508	1
TATCAAC	2985	0.0	28.308695	2
GTAGCAC	85	0.007198782	28.003796	3
AACGCAG	3180	0.0	27.130274	6
TCAACGC	3175	0.0	26.985598	4
TCTAACG	90	0.009513689	26.448029	2
ACGCAGA	3300	0.0	25.963415	7
ATGGGTA	120	0.0012861169	24.791456	5
CGCAGAG	3495	0.0	24.514812	8
GTAGGAC	175	1.7443588E-5	23.803226	3
TTTAGGG	280	2.586603E-9	23.37817	2
GGCTATT	255	2.466004E-8	23.333136	7
GCAGAGT	3720	0.0	23.032063	9
>>END_MODULE