FastQCFastQC Report
Sun 1 Jan 2017
ERR1142835_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142835_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645808
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29630.45880509377400097No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.046.3701441
GTATCAA25400.040.354231
ATCAACG32200.030.5083033
CAACGCA32800.030.313265
TCAACGC32850.030.267124
TATCAAC33450.030.2581792
AACGCAG33300.029.8303456
ACGCAGA37700.026.5065967
CGCAGAG38600.026.1987958
GTACATG54650.026.0616591
TACATGG56250.025.08512
CATGGGT9850.024.7820724
GCAGAGT40800.024.6403129
ACATGGG55900.024.6031173
CATGGGG31650.024.0783234
AGAGTAC39150.021.57626310-11
CAGAGTA40450.021.32402410-11
ATGGGGG20650.020.7588085
ATGGGGC7000.020.4128285
AGTACAC6300.019.8458043
Produced by FastQC (version 0.11.5)