FastQCFastQC Report
Sun 1 Jan 2017
ERR1142835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645808
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.5562024626514381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.5049488392835022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.16289671233555486No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.1467928548423061No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.129140549513168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA3900.071.726081
GGTATCA23950.057.1565741
GTCAACG1500.051.5817381
GTATCAA36650.040.4360961
GCGTAAG957.4522195E-637.589931
ATCAACG43650.033.8046723
TATCAAC44650.033.0475732
CAACGCA45050.033.0182885
TCAACGC45250.032.8723534
AACGCAG47750.031.6497086
GTGGTAT10950.028.80751
ACGCAGA52150.028.637087
CGCAGAG54900.027.202628
TGGTATC12300.025.6377582
GCAGAGT59700.024.915819
CAGAGTA58550.024.54141210-11
GTACATG50250.024.3991681
CATGGGT8400.024.082914
CATGGGG28350.023.9255074
ACATGGG50500.023.9174023
Produced by FastQC (version 0.11.5)