##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	645808
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.400225763694472	27.0	14.0	33.0	14.0	33.0
2	24.105881314570276	27.0	14.0	27.0	14.0	33.0
3	23.898164779624903	27.0	14.0	27.0	14.0	33.0
4	29.664183472487178	33.0	27.0	33.0	27.0	33.0
5	31.565039454450858	33.0	33.0	33.0	27.0	33.0
6	35.58341643336719	37.0	37.0	37.0	33.0	37.0
7	35.637742177241535	37.0	37.0	37.0	33.0	37.0
8	35.977750353046105	37.0	37.0	37.0	33.0	37.0
9	36.15901785050665	37.0	37.0	37.0	37.0	37.0
10-11	36.078742443574555	37.0	37.0	37.0	37.0	37.0
12-13	36.16562352897456	37.0	37.0	37.0	37.0	37.0
14-15	36.15077623690013	37.0	37.0	37.0	37.0	37.0
16-17	36.158184785570946	37.0	37.0	37.0	37.0	37.0
18-19	36.14831652751282	37.0	37.0	37.0	37.0	37.0
20-21	36.0831578425786	37.0	37.0	37.0	37.0	37.0
22-23	36.12652522111836	37.0	37.0	37.0	37.0	37.0
24-25	36.146324449371946	37.0	37.0	37.0	37.0	37.0
26-27	36.08597059807249	37.0	37.0	37.0	37.0	37.0
28-29	36.08232555186681	37.0	37.0	37.0	37.0	37.0
30-31	36.05445116814904	37.0	37.0	37.0	37.0	37.0
32-33	36.00924423358026	37.0	37.0	37.0	37.0	37.0
34-35	35.946168210985306	37.0	37.0	37.0	37.0	37.0
36-37	35.88695711418873	37.0	37.0	37.0	35.0	37.0
38-39	35.83626635160915	37.0	37.0	37.0	33.0	37.0
40-41	35.77248113990536	37.0	37.0	37.0	33.0	37.0
42-43	35.7083823675148	37.0	37.0	37.0	33.0	37.0
44-45	35.627369125188906	37.0	37.0	37.0	33.0	37.0
46-47	35.508212193097634	37.0	37.0	37.0	33.0	37.0
48-49	35.29376532963357	37.0	37.0	37.0	33.0	37.0
50-51	35.239546428659914	37.0	37.0	37.0	33.0	37.0
52-53	35.21197012115056	37.0	37.0	37.0	33.0	37.0
54-55	35.149035007308676	37.0	37.0	37.0	33.0	37.0
56-57	35.124670180610956	37.0	37.0	37.0	33.0	37.0
58-59	35.08531018507048	37.0	37.0	37.0	33.0	37.0
60-61	35.04654169660333	37.0	37.0	37.0	33.0	37.0
62-63	35.02255314272973	37.0	37.0	37.0	33.0	37.0
64-65	35.005671190198946	37.0	37.0	37.0	33.0	37.0
66-67	34.97169901890345	37.0	37.0	37.0	27.0	37.0
68-69	34.89208634764512	37.0	37.0	37.0	27.0	37.0
70-71	34.87812399350891	37.0	37.0	37.0	27.0	37.0
72-73	34.864273127616876	37.0	37.0	37.0	27.0	37.0
74-75	34.82340107276466	37.0	37.0	37.0	27.0	37.0
76-77	34.76543028268463	37.0	37.0	37.0	27.0	37.0
78-79	34.68807215147536	37.0	37.0	37.0	27.0	37.0
80-81	34.59979514035131	37.0	37.0	37.0	27.0	37.0
82-83	34.60555149518123	37.0	37.0	37.0	27.0	37.0
84-85	34.57006726457399	37.0	37.0	37.0	27.0	37.0
86-87	34.50299237544286	37.0	37.0	37.0	27.0	37.0
88-89	34.45608911626985	37.0	37.0	37.0	27.0	37.0
90-91	34.381506577806405	37.0	37.0	37.0	27.0	37.0
92-93	34.29796472016451	37.0	37.0	37.0	27.0	37.0
94-95	34.246784648068775	37.0	37.0	37.0	27.0	37.0
96-97	34.1765842169809	37.0	37.0	37.0	27.0	37.0
98-99	34.0944057676585	37.0	37.0	37.0	27.0	37.0
100-101	34.02696621906201	37.0	37.0	37.0	22.0	37.0
102-103	33.94342126452444	37.0	37.0	37.0	22.0	37.0
104-105	33.784289603101854	37.0	33.0	37.0	22.0	37.0
106-107	33.730601045511975	37.0	33.0	37.0	22.0	37.0
108-109	33.66466503976414	37.0	33.0	37.0	22.0	37.0
110-111	33.6178895275376	37.0	33.0	37.0	22.0	37.0
112-113	33.53749953546565	37.0	33.0	37.0	22.0	37.0
114-115	33.385345179991575	37.0	33.0	37.0	22.0	37.0
116-117	33.346191902237194	37.0	33.0	37.0	22.0	37.0
118-119	33.190798968114365	37.0	33.0	37.0	14.0	37.0
120-121	33.089717841835345	37.0	33.0	37.0	14.0	37.0
122-123	33.00466160220994	37.0	33.0	37.0	14.0	37.0
124-125	31.348351212744348	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	279.0
16	146.0
17	40.0
18	98.0
19	349.0
20	1366.0
21	3064.0
22	5032.0
23	6530.0
24	5984.0
25	5218.0
26	5199.0
27	5858.0
28	7478.0
29	9649.0
30	12714.0
31	17281.0
32	24600.0
33	37035.0
34	62857.0
35	146464.0
36	288561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	58.09128759247098	10.999708801625754	10.880751167891352	20.028252438011922
2	18.35941189677999	20.004025983075387	35.07962929986606	26.55693282027857
3	22.495764982053434	25.16312948470593	27.740374044217614	24.60073148902302
4	15.327023787771331	17.616298695868963	34.96811676788573	32.088560748473974
5	15.86355549155235	35.9716073527294	31.283166176577616	16.881670979140633
6	30.047785100215545	33.9679904863365	18.777872061046008	17.20635235240195
7	26.71304784084434	32.25246512895474	22.348747615390334	18.68573941481059
8	26.369292421276914	31.40763198969353	21.16077843569606	21.062297153333496
9	27.72712632856824	15.92826350865892	20.70878651239997	35.63582365037286
10-11	25.84374922577608	24.86420112479251	26.346065703738574	22.945983945692838
12-13	26.930941281856533	23.924231057183626	26.491799775629037	22.6530278853308
14-15	25.364718379266854	24.145158031082065	25.140967147280186	25.349156442370894
16-17	23.978256695488444	26.180226940514828	26.067964472412854	23.773551891583878
18-19	23.697054533404767	25.020516997364535	28.500741708810384	22.78168676042031
20-21	25.140754344183858	23.832002180221306	28.448249164609805	22.57899431098503
22-23	25.238306121943367	23.58046044644848	28.31228476575081	22.868948665857346
24-25	24.224225231725935	24.17955237331335	28.39893094830336	23.197291446657356
26-27	23.6982932955098	24.436982623264573	28.51924571775425	23.345478363471383
28-29	23.460378316775266	24.561402150484355	28.792381017268294	23.185838515472092
30-31	24.213866911529593	24.10121654651918	28.779264900236683	22.90565164171455
32-33	23.748621881426057	24.221595272898448	28.49747912692317	23.532303718752324
34-35	23.919802789683622	24.52609753982608	28.60966417263335	22.94443549785695
36-37	23.961895589145012	24.5027713495338	28.338529694357966	23.196803366963223
38-39	23.87698820702128	24.418015880881004	28.33535663850556	23.369639273592153
40-41	23.569079876958714	24.402465471094356	28.5958722482752	23.432582403671727
42-43	23.81073307448427	24.430112688378504	28.208018643326376	23.551135593810848
44-45	23.671664023982363	24.56566038203305	28.106031514010354	23.656644079974235
46-47	23.6585022173773	24.45285595718851	27.960941951787525	23.927699873646656
48-49	23.841761018754802	24.849258603176178	27.81763310457597	23.49134727349305
50-51	23.60951212242038	24.924300197814365	27.649338231593777	23.816849448171475
52-53	23.7704841879025	24.73525208151712	27.538970862631484	23.955292867948895
54-55	23.50868988925501	24.60483611228105	27.73595248123281	24.150521517231127
56-57	23.625752545648243	24.510922751034364	27.307884077001216	24.55544062631618
58-59	23.60732601640116	24.772378168124273	27.517156802021653	24.103139013452914
60-61	23.851593662512695	24.6782325397022	27.819026707628275	23.651147090156826
62-63	23.431226357100496	24.51297369028208	27.946971773300692	24.10882817931673
64-65	23.332786215097986	24.345006565418824	28.07560451403513	24.24660270544806
66-67	23.56505339048138	24.394556896167284	27.931211753338452	24.109177960012886
68-69	23.530213314173874	24.293365830091915	27.907597923841145	24.26882293189307
70-71	23.31188216931348	24.39192453484627	28.154033396922923	24.142159898917328
72-73	23.53067784852464	24.70331739464361	28.02311213239848	23.742892624433267
74-75	23.355703243069147	24.38604043306989	28.328853157594825	23.929403166266138
76-77	23.314764848658463	24.246466632858862	28.242936169059668	24.19583234942301
78-79	23.23538884622055	24.554434135222852	28.177492381636647	24.032684636919953
80-81	23.193503332259745	24.685897356489857	28.261495676733645	23.85910363451676
82-83	23.356942001337856	24.417628768922032	28.55121026682853	23.674218962911578
84-85	23.209607189752994	24.65670911478334	28.073281842281293	24.06040185318237
86-87	22.85385129945742	25.037472437628523	28.013202066248795	24.09547419666526
88-89	23.02960012883086	24.399976463592896	28.21380348338825	24.356619924187996
90-91	23.47555310556698	24.219814557887172	27.97038748358645	24.334244852959394
92-93	23.45944924807373	24.53933676882293	27.78426405371256	24.21694992939078
94-95	22.89976277779154	24.53894965686396	28.029847880484603	24.531439684859897
96-97	22.953493917696903	24.550950127592102	27.81678145826623	24.678774496444763
98-99	22.739396398147125	24.592583072483787	27.689563359922825	24.978457169446266
100-101	23.155000762612328	24.858994141434003	27.325754695872522	24.66025040008114
102-103	23.153106769432068	24.76016460013239	27.60660103823508	24.48012759220046
104-105	23.279204590852363	24.659650111643437	27.484492246897673	24.576653050606527
106-107	23.240243013967806	24.478539624485045	27.525969466086202	24.755247895460947
108-109	23.242005738556564	24.545723412722374	27.303590701246655	24.9086801474744
110-111	22.921923328546047	24.99816121677125	27.252780433797994	24.827135020884704
112-113	22.946322806419893	25.143580492563544	27.17987627844318	24.730220422573375
114-115	22.972551333180036	25.225164542575122	27.082534911827167	24.71974921241767
116-117	22.832106570786408	25.093004008172738	27.104910069672677	24.969979351368174
118-119	22.726171203291422	24.68482865037833	27.438237409598493	25.150762736731757
120-121	22.88793951096542	24.716360871659006	27.666563952164978	24.729135665210595
122-123	22.76082915306256	25.08603783109564	27.380896156112307	24.772236859729492
124-125	22.695958501083926	24.807680396407555	27.25332920408795	25.243031898420565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	202.0
1	194.0
2	487.5
3	776.0
4	610.0
5	521.5
6	640.5
7	1101.0
8	1989.5
9	2539.5
10	2213.5
11	1627.5
12	1363.5
13	1189.5
14	1096.5
15	1071.0
16	1030.0
17	1029.5
18	965.0
19	895.5
20	901.0
21	892.5
22	895.5
23	933.5
24	987.5
25	1101.5
26	1289.5
27	1507.5
28	1816.5
29	2464.0
30	3402.5
31	4404.0
32	5577.5
33	6983.5
34	8451.5
35	9864.5
36	11153.0
37	12429.0
38	13460.0
39	14231.5
40	14417.5
41	13999.0
42	14355.5
43	15542.5
44	17195.5
45	18785.5
46	20516.5
47	22425.0
48	24071.5
49	25490.0
50	26262.5
51	26549.0
52	26668.0
53	26995.0
54	27105.5
55	26462.5
56	25641.0
57	23767.5
58	21425.5
59	18998.0
60	16185.0
61	13384.0
62	10856.0
63	8728.5
64	6779.5
65	5363.0
66	4133.0
67	3191.5
68	2659.5
69	2249.0
70	1931.5
71	1749.5
72	1517.5
73	1416.5
74	1193.0
75	998.0
76	803.0
77	540.0
78	385.0
79	249.0
80	180.0
81	141.0
82	105.0
83	69.5
84	50.0
85	29.5
86	20.5
87	13.5
88	7.0
89	5.5
90	4.0
91	1.5
92	2.0
93	2.5
94	1.0
95	0.5
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030968956717786093
2	4.645343507667914E-4
3	3.096895671778609E-4
4	0.0021678269702450266
5	0.0010839134851225133
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	3.871119589723261E-4
14-15	2.322671753833957E-4
16-17	0.0
18-19	3.096895671778609E-4
20-21	3.096895671778609E-4
22-23	0.0
24-25	3.096895671778609E-4
26-27	3.096895671778609E-4
28-29	0.0
30-31	6.96801526150187E-4
32-33	0.0
34-35	0.0
36-37	5.419567425612566E-4
38-39	0.0
40-41	0.0013161806605059088
42-43	7.742239179446523E-5
44-45	0.0
46-47	0.0
48-49	0.0
50-51	7.742239179446523E-5
52-53	7.742239179446522E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	1.5484478358893046E-4
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	7.742239179446523E-5
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	2.322671753833957E-4
100-101	2.322671753833957E-4
102-103	7.742239179446523E-5
104-105	3.096895671778609E-4
106-107	2.322671753833957E-4
108-109	1.5484478358893046E-4
110-111	7.742239179446523E-5
112-113	4.645343507667914E-4
114-115	3.871119589723261E-4
116-117	3.871119589723261E-4
118-119	0.0013161806605059088
120-121	7.742239179446522E-4
122-123	0.0025549389292173525
124-125	0.0012387582687114437
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	645808.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.66904717147237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.62978427014147	66.30862279563283
2	8.526688332089494	12.90412763234646
3	1.8954626279961029	4.302835530288003
4	0.6602237187136253	1.9983399886026492
5	0.33108424728043057	1.2526414762597162
6	0.20242345270246814	0.9190313874693221
7	0.13397279726211006	0.7096315741004497
8	0.09329765249413952	0.5647795574053346
9	0.07379857038852099	0.5025840753524596
>10	0.41259608073543846	5.634988767820263
>50	0.02598918941481853	1.300854102583472
>100	0.01323521873734588	1.7234842437148516
>500	7.219210220343591E-4	0.43865541923927565
>1k	7.219210220343591E-4	1.4394234491849205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3592	0.5562024626514381	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3261	0.5049488392835022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1052	0.16289671233555486	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	948	0.1467928548423061	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	834	0.129140549513168	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
2	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
3	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
4	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
5	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
6	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
7	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
8	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
9	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
10-11	0.0	0.0	0.0	1.5484478358893046E-4	1.5484478358893046E-4
12-13	0.0	0.0	0.0	3.096895671778609E-4	1.5484478358893046E-4
14-15	0.0	0.0	0.0	3.096895671778609E-4	4.645343507667914E-4
16-17	0.0	0.0	0.0	3.871119589723261E-4	4.645343507667914E-4
18-19	0.0	0.0	0.0	5.419567425612566E-4	6.193791343557218E-4
20-21	0.0	0.0	0.0	8.516463097391176E-4	6.193791343557218E-4
22-23	0.0	0.0	0.0	0.0026323613210118176	6.193791343557218E-4
24-25	0.0	0.0	0.0	0.004490498724078983	6.193791343557218E-4
26-27	0.0	0.0	0.0	0.007045437653296335	6.96801526150187E-4
28-29	0.0	0.0	0.0	0.013858608131209276	8.516463097391176E-4
30-31	0.0	0.0	0.0	0.029188241706513393	9.290687015335828E-4
32-33	0.0	0.0	0.0	0.055047320565864775	9.290687015335828E-4
34-35	0.0	0.0	0.0	0.08268711443648886	9.290687015335828E-4
36-37	0.0	0.0	0.0	0.1286760151624012	9.290687015335828E-4
38-39	0.0	0.0	0.0	0.1957238064564081	0.0010839134851225133
40-41	0.0	0.0	0.0	0.2678814756088497	0.0012387582687114437
42-43	0.0	0.0	0.0	0.3332259742833783	0.0013161806605059088
44-45	0.0	0.0	0.0	0.40027376557738525	0.001393603052300374
46-47	0.0	0.0	0.0	0.48776106830513094	0.001393603052300374
48-49	0.0	0.0	0.0	0.582293808686173	0.001393603052300374
50-51	0.0	0.0	0.0	0.672336050343136	0.001393603052300374
52-53	0.0	0.0	0.0	0.7539392512945025	0.0016258702276837698
54-55	0.0	0.0	0.0	0.8395684166191809	0.0017032926194782351
56-57	0.0	0.0	0.0	0.9313139508956223	0.0017032926194782351
58-59	0.0	0.0	0.0	1.0418731263781185	0.0017032926194782351
60-61	0.0	0.0	0.0	1.1480192255283304	0.0017032926194782351
62-63	0.0	0.0	0.0	1.2490554468201074	0.0017032926194782351
64-65	0.0	0.0	0.0	1.3418848945816713	0.0017032926194782351
66-67	0.0	0.0	0.0	1.4559280776949186	0.0017032926194782351
68-69	0.0	0.0	0.0	1.5790296806481183	0.0017032926194782351
70-71	0.0	0.0	0.0	1.7313195253078315	0.0017032926194782351
72-73	0.0	0.0	0.0	1.865724797463023	0.0017032926194782351
74-75	0.0	0.0	0.0	1.9891360899834005	0.0017032926194782351
76-77	0.0	0.0	0.0	2.1342256522062284	0.0017032926194782351
78-79	0.0	0.0	0.0	2.2896898149295146	0.0017032926194782351
80-81	0.0	0.0	0.0	2.4620320590639944	0.0017032926194782351
82-83	0.0	0.0	0.0	2.646065084359438	0.0017032926194782351
84-85	0.0	0.0	0.0	2.816471768699056	0.0017032926194782351
86-87	0.0	0.0	0.0	2.9890462800089193	0.0017032926194782351
88-89	0.0	0.0	0.0	3.183221638629438	0.0017032926194782351
90-91	0.0	0.0	0.0	3.3965977504149842	0.0018581374030671655
92-93	0.0	0.0	0.0	3.607573768054902	0.0018581374030671655
94-95	0.0	0.0	0.0	3.8180078289522585	0.0018581374030671655
96-97	0.0	0.0	0.0	4.025809528528603	0.0018581374030671655
98-99	0.0	0.0	0.0	4.2528894036617695	0.0018581374030671655
100-101	0.0	0.0	0.0	4.507531650273766	0.0018581374030671655
102-103	0.0	0.0	0.0	4.767980576270347	0.002012982186656096
104-105	0.0	0.0	0.0	5.027113321606421	0.002012982186656096
106-107	0.0	0.0	0.0	5.311485766667493	0.002012982186656096
108-109	0.0	0.0	0.0	5.597793771523425	0.002012982186656096
110-111	0.0	0.0	0.0	5.890140722939326	0.002012982186656096
112-113	0.0	0.0	0.0	6.207262839729455	0.002012982186656096
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAGA	390	0.0	71.72608	1
GGTATCA	2395	0.0	57.156574	1
GTCAACG	150	0.0	51.581738	1
GTATCAA	3665	0.0	40.436096	1
GCGTAAG	95	7.4522195E-6	37.58993	1
ATCAACG	4365	0.0	33.804672	3
TATCAAC	4465	0.0	33.047573	2
CAACGCA	4505	0.0	33.018288	5
TCAACGC	4525	0.0	32.872353	4
AACGCAG	4775	0.0	31.649708	6
GTGGTAT	1095	0.0	28.8075	1
ACGCAGA	5215	0.0	28.63708	7
CGCAGAG	5490	0.0	27.20262	8
TGGTATC	1230	0.0	25.637758	2
GCAGAGT	5970	0.0	24.91581	9
CAGAGTA	5855	0.0	24.541412	10-11
GTACATG	5025	0.0	24.399168	1
CATGGGT	840	0.0	24.08291	4
CATGGGG	2835	0.0	23.925507	4
ACATGGG	5050	0.0	23.917402	3
>>END_MODULE