FastQCFastQC Report
Sun 1 Jan 2017
ERR1142834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277751
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.2120604426266692No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3850.13861336232812843No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3290.11845141871676429No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC700.002776868234.0211754
ACGCAAG700.002784242434.002788
TATATAC850.00717923628.0174372
AGGGCTA1556.774044E-626.8877035
GTACTAG1807.149538E-726.489571
AGTACTC1401.04864754E-425.515885
CATGGGT2901.4370016E-1024.6360234
CTAGTAC1451.3308608E-424.6360233
CATGGGG11050.023.1682644
GTATTTA1552.0765411E-423.071561
AATAGTG1350.002549234522.038847
GACGCTA700.003152133421.2517412-13
ATGGGGG7250.020.5300185
GTATCAA22600.020.0429941
CTATTGA1550.005674845519.1951189
AACGCAG24100.019.0119296
GTACTGT1908.295425E-418.7910126
ATCAACG23600.018.6683983
CCTAGTA1600.0067954518.605332
ACGCAGA24900.018.4011047
Produced by FastQC (version 0.11.5)