##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142834_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277751
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.652224474439336	33.0	33.0	33.0	27.0	33.0
2	30.769750603958222	33.0	33.0	33.0	27.0	33.0
3	30.872842942059613	33.0	33.0	33.0	27.0	33.0
4	30.806070905235266	33.0	33.0	33.0	27.0	33.0
5	30.919683457485302	33.0	33.0	33.0	27.0	33.0
6	34.002797469676075	37.0	37.0	37.0	27.0	37.0
7	34.03801246440157	37.0	37.0	37.0	27.0	37.0
8	33.967686885015716	37.0	37.0	37.0	27.0	37.0
9	33.91734323188756	37.0	37.0	37.0	22.0	37.0
10-11	33.99339516329374	37.0	37.0	37.0	27.0	37.0
12-13	33.9365240809214	37.0	37.0	37.0	24.5	37.0
14-15	33.9201443019107	37.0	37.0	37.0	24.5	37.0
16-17	33.94909109238131	37.0	37.0	37.0	27.0	37.0
18-19	33.931204568120364	37.0	37.0	37.0	27.0	37.0
20-21	33.91263757826255	37.0	37.0	37.0	27.0	37.0
22-23	33.987688613182314	37.0	37.0	37.0	27.0	37.0
24-25	34.0914866193101	37.0	37.0	37.0	27.0	37.0
26-27	34.128730409611485	37.0	37.0	37.0	27.0	37.0
28-29	34.20700015481492	37.0	37.0	37.0	27.0	37.0
30-31	34.21952756245702	37.0	37.0	37.0	27.0	37.0
32-33	34.26589103189548	37.0	37.0	37.0	27.0	37.0
34-35	34.27090451519527	37.0	37.0	37.0	27.0	37.0
36-37	34.25512779431938	37.0	37.0	37.0	27.0	37.0
38-39	34.219435753606646	37.0	37.0	37.0	27.0	37.0
40-41	34.20280034995373	37.0	37.0	37.0	27.0	37.0
42-43	34.17096788130375	37.0	37.0	37.0	27.0	37.0
44-45	34.15767179956148	37.0	37.0	37.0	27.0	37.0
46-47	34.149855446065004	37.0	37.0	37.0	27.0	37.0
48-49	34.09880972525751	37.0	37.0	37.0	27.0	37.0
50-51	34.071043848627006	37.0	37.0	37.0	27.0	37.0
52-53	34.02926722136014	37.0	37.0	37.0	27.0	37.0
54-55	34.01040320286876	37.0	37.0	37.0	27.0	37.0
56-57	33.95999474349327	37.0	37.0	37.0	27.0	37.0
58-59	33.88136316340895	37.0	37.0	37.0	27.0	37.0
60-61	33.86005630942823	37.0	37.0	37.0	27.0	37.0
62-63	33.82205284589435	37.0	37.0	37.0	27.0	37.0
64-65	33.761772234843434	37.0	37.0	37.0	22.0	37.0
66-67	33.72545913426055	37.0	37.0	37.0	22.0	37.0
68-69	33.6566240985631	37.0	33.0	37.0	22.0	37.0
70-71	33.58187909314458	37.0	33.0	37.0	18.0	37.0
72-73	33.524151128168754	37.0	33.0	37.0	14.0	37.0
74-75	33.46405773516567	37.0	33.0	37.0	14.0	37.0
76-77	33.38433705009163	37.0	33.0	37.0	14.0	37.0
78-79	33.32054970099118	37.0	33.0	37.0	14.0	37.0
80-81	33.240623796133946	37.0	33.0	37.0	14.0	37.0
82-83	33.153581805286024	37.0	33.0	37.0	14.0	37.0
84-85	33.0478702146887	37.0	33.0	37.0	14.0	37.0
86-87	32.97990646298303	37.0	33.0	37.0	14.0	37.0
88-89	32.87222908288359	37.0	33.0	37.0	14.0	37.0
90-91	32.75498558060997	37.0	33.0	37.0	14.0	37.0
92-93	32.69614870873552	37.0	33.0	37.0	14.0	37.0
94-95	32.63856835798971	37.0	33.0	37.0	14.0	37.0
96-97	32.5138703371005	37.0	33.0	37.0	14.0	37.0
98-99	32.39222001000897	37.0	33.0	37.0	14.0	37.0
100-101	32.27013224074801	37.0	33.0	37.0	14.0	37.0
102-103	32.17931528599357	37.0	33.0	37.0	14.0	37.0
104-105	32.00874704321497	37.0	33.0	37.0	14.0	37.0
106-107	31.953136082318338	37.0	30.0	37.0	14.0	37.0
108-109	31.833257125986947	37.0	27.0	37.0	14.0	37.0
110-111	31.68203894855464	37.0	27.0	37.0	14.0	37.0
112-113	31.535935064140183	37.0	27.0	37.0	14.0	37.0
114-115	31.391339725149503	37.0	27.0	37.0	14.0	37.0
116-117	31.28854441568167	37.0	27.0	37.0	14.0	37.0
118-119	31.155210962336767	37.0	27.0	37.0	14.0	37.0
120-121	31.070997044115053	37.0	27.0	37.0	14.0	37.0
122-123	30.84046862117508	37.0	27.0	37.0	14.0	37.0
124-125	28.997951402515206	35.0	18.0	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	153.0
1	16.0
2	10.0
3	22.0
4	11.0
5	9.0
6	16.0
7	10.0
8	18.0
9	13.0
10	10.0
11	6.0
12	7.0
13	7.0
14	29.0
15	98.0
16	162.0
17	442.0
18	1123.0
19	2238.0
20	3365.0
21	4055.0
22	4454.0
23	4538.0
24	4487.0
25	4899.0
26	5545.0
27	6067.0
28	6943.0
29	7977.0
30	9328.0
31	10881.0
32	13179.0
33	16761.0
34	23548.0
35	40407.0
36	106917.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.579330474577496	20.467730892580445	11.980829519656949	23.972109113185113
2	17.18658755506211	23.74141175283151	35.2332614792332	23.83873921287318
3	19.4835960063604	28.588993333116992	27.769424643309137	24.157986017213467
4	14.370510342440065	20.597971900874132	33.44921097423766	31.582306782448143
5	15.046782233572115	38.50967746589622	30.816037974044335	15.627502326487328
6	29.170525678495363	37.10381312412384	17.739081549178525	15.98657964820227
7	25.705967347321163	34.438451033593125	21.321168751148537	18.534412867937174
8	25.129073516960602	34.72681090234368	20.254363999927943	19.889751580767776
9	25.880178287674443	19.443373485293428	19.650919363381846	35.02552886365029
10-11	24.8272768380717	28.32803979253404	24.962032431419672	21.882650937974596
12-13	25.444914872534007	27.32600666606612	24.685343662733086	22.543734798666787
14-15	24.223631580843932	27.15505747540629	23.090879607942057	25.53043133580772
16-17	23.711715412569976	28.91043762308093	23.919815646615174	23.458031317733923
18-19	23.772568380842554	28.0038920321453	25.454430790298748	22.769108796713393
20-21	25.564634552841863	26.696336069044424	24.69058837307772	23.04844100503599
22-23	25.6285427034273	26.431044982649087	24.396501709866776	23.543910604056833
24-25	24.46928481354711	27.09764006485318	24.695370203566924	23.737704918032787
26-27	24.28200503438844	26.892895818430475	24.77211013670689	24.052989010474192
28-29	23.938636531554828	26.775967116158455	25.330361149204485	23.95503520308223
30-31	24.557916418136376	26.460321951096443	25.277287725096365	23.704473905670813
32-33	23.827895527820925	26.98300002522786	24.999369303458742	24.18973514349247
34-35	24.115807779990448	26.79131711976355	25.231174027050653	23.861701073195345
36-37	24.033126771527265	27.212100353359435	24.680742180953906	24.074030694159386
38-39	24.23027509545544	27.048079989765235	24.84904616637626	23.87259874840307
40-41	23.81955608099828	26.892855340652687	25.217345857452838	24.0702427208962
42-43	24.112984330561353	27.003197329671004	24.997566857352957	23.886251482414686
44-45	24.0316644377075	27.132320869185932	24.9618793910796	23.87413530202697
46-47	23.91333340542333	26.960577585057184	24.95629543922633	24.169793570293155
48-49	24.092659639460724	27.118118693581263	25.077898181805075	23.711323485152942
50-51	23.695455880789186	27.228669421770608	25.064657231066473	24.011217466373736
52-53	23.524566377615116	27.178475963540212	24.82448725042943	24.47247040841524
54-55	23.68758717881253	26.957220658359976	24.834653104917336	24.52053905791016
56-57	23.998255484949954	26.60529626118706	24.709217455242737	24.687230798620245
58-59	23.81025334160022	26.63853762634637	25.1712254696985	24.37998356235491
60-61	24.051987932917402	26.786724767434734	25.13903456130676	24.022252738341102
62-63	23.414440719512637	26.910349302476476	25.086154067986016	24.589055910024875
64-65	23.29231501205679	26.767338170467532	25.094706185548432	24.84564063192725
66-67	24.03070318512204	26.323349451484248	24.99040300183289	24.655544361560825
68-69	23.755793920258355	26.271598797603858	24.897277308016697	25.07532997412109
70-71	23.61011233727496	26.26814238880268	25.19901882861401	24.922726445308346
72-73	24.030890396763176	26.121489010840477	25.358420516702264	24.489200075694086
74-75	24.098033843558262	26.063289040137853	25.22296805266156	24.615709063642328
76-77	23.832571722142607	26.160127626206638	25.355613840593428	24.65168681105733
78-79	23.74650304271335	26.165171747469213	25.38142070198714	24.7069045078303
80-81	23.586839159502997	26.14701307035206	25.489619966580563	24.77652780356438
82-83	23.957877052475226	25.892598020394512	25.70242466521378	24.44710026191648
84-85	23.950294121358638	26.30705080015792	25.069451580395274	24.673203498088167
86-87	23.53013281802056	26.38207894091689	25.377291146907403	24.710497094155144
88-89	24.022113748449804	25.783210278891357	25.371867158884438	24.822808813774408
90-91	24.147087614044636	25.834614767530113	25.325706621574607	24.692590996850647
92-93	23.832246596925653	26.146571507375803	25.492186133880395	24.528995761818145
94-95	22.995581690062135	26.300030464886966	25.837288661113284	24.86709918393761
96-97	23.375710288730566	26.504946782428107	25.21597104041074	24.903371888430588
98-99	23.13538601350182	26.731723501240552	25.19293462581501	24.939955859442616
100-101	23.402299347833143	26.821218315469945	25.020819588799604	24.75566274789731
102-103	23.394865878280935	27.041029708681858	25.23056677242573	24.333537640611482
104-105	23.336897007415207	27.142215836054167	25.29012694011405	24.230760216416577
106-107	23.372762396176444	27.188204814780757	25.21801369109754	24.221019097945256
108-109	23.285757295503263	27.31845297652206	24.976248037195482	24.41954169077919
110-111	23.12522082735447	27.813791164021545	24.750510156255633	24.31047785236835
112-113	23.037980160747704	27.769905058612693	24.743896238763348	24.448218541876255
114-115	23.04624537996935	27.713873613990803	24.810060398449473	24.429820607590372
116-117	23.2261518880544	27.649820262557284	24.792409563402067	24.33161828598625
118-119	22.93981055812159	27.46258216419498	25.04191590857464	24.555691369108786
120-121	22.965122043106696	27.53932760476095	25.28016468293668	24.215385669195673
122-123	22.908303696800697	27.868314656159143	25.24780227010132	23.975579376938843
124-125	23.03535593030866	27.554871199404275	24.936487702324307	24.473285167962754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	348.0
1	282.5
2	148.0
3	80.0
4	61.5
5	45.0
6	46.0
7	41.5
8	51.0
9	58.5
10	49.0
11	50.0
12	51.0
13	48.5
14	58.5
15	77.5
16	94.5
17	115.5
18	136.5
19	166.5
20	216.0
21	258.5
22	295.0
23	383.5
24	515.0
25	633.0
26	729.0
27	888.0
28	1117.5
29	1453.0
30	1900.0
31	2374.0
32	2916.5
33	3426.0
34	3882.0
35	4378.0
36	4823.0
37	5565.5
38	6126.0
39	6313.5
40	6414.0
41	6450.5
42	6743.0
43	7417.5
44	8251.5
45	9064.5
46	10109.5
47	11134.0
48	11919.5
49	12456.0
50	12906.0
51	12695.5
52	12056.0
53	11916.5
54	11471.5
55	10818.5
56	10275.5
57	9049.0
58	7925.0
59	7078.5
60	6085.0
61	5105.0
62	4084.0
63	3275.0
64	2579.0
65	1991.0
66	1558.5
67	1214.0
68	1011.0
69	822.5
70	683.0
71	616.0
72	539.0
73	487.5
74	378.5
75	293.0
76	247.0
77	184.5
78	130.0
79	79.0
80	60.0
81	56.0
82	37.0
83	20.0
84	13.0
85	9.0
86	7.5
87	3.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08676836447033494
2	0.12133169637553061
3	0.14761423001177315
4	0.1609355141835673
5	0.18325766603900617
6	0.09432909332459649
7	0.08064777444545654
8	0.07056680263977448
9	0.07992770503076496
10-11	0.07614734060363419
12-13	0.08136784386014811
14-15	0.08676836447033494
16-17	0.08658834711666205
18-19	0.0939690586172507
20-21	0.08946862477542836
22-23	0.0878484685923723
24-25	0.07236697617650341
26-27	0.09342900655623201
28-29	0.10423004777660566
30-31	0.1031499436545683
32-33	0.10080971805682068
34-35	0.11107070721617565
36-37	0.09774942304438149
38-39	0.09558921480030674
40-41	0.11269086339923168
42-43	0.11953152283880165
44-45	0.12313186991225955
46-47	0.11521110635065221
48-49	0.10999060309413829
50-51	0.11755133194839983
52-53	0.12583213021735296
54-55	0.10945055103311958
56-57	0.11161075927719431
58-59	0.12205176579022219
60-61	0.10873048161842802
62-63	0.12277183520491375
64-65	0.11305089810657748
66-67	0.1153911237043251
68-69	0.10981058574046539
70-71	0.11791136665574563
72-73	0.11467105428963353
74-75	0.12853239052244636
76-77	0.1362731367303808
78-79	0.13213273759590424
80-81	0.13231275494957714
82-83	0.13411292848630607
84-85	0.14383386558464237
86-87	0.1364531540840537
88-89	0.13213273759590424
90-91	0.14131362263322184
92-93	0.14095358792587606
94-95	0.13771327555976398
96-97	0.14365384823096947
98-99	0.1638157918423336
100-101	0.13249277230325002
102-103	0.14077357057220316
104-105	0.14959442090217498
106-107	0.15031449031686653
108-109	0.1459940738287171
110-111	0.13861336232812843
112-113	0.15193464649992258
114-115	0.15337478532930576
116-117	0.14473395235300682
118-119	0.14761423001177315
120-121	0.13303282436426872
122-123	0.1305125814128482
124-125	0.15931535800051125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	277751.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.86955031195282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64740784585892	63.86827195736065
2	8.33508042541621	12.147471248280794
3	1.7314212937142224	3.7850367322048495
4	0.6416120401648981	1.8701592336620296
5	0.32816623122794253	1.195666284857425
6	0.18150483421827476	0.7935705389358737
7	0.1466038244610275	0.7478068333741279
8	0.1304360732024494	0.7603854398975559
9	0.09963410393182132	0.653426311432157
>10	0.6934529574079048	9.896177986426869
>50	0.050247560473181914	2.4426993574004157
>100	0.01389826140747585	1.609898970520797
>500	5.34548515672148E-4	0.22942910564644312
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	589	0.2120604426266692	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	385	0.13861336232812843	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	329	0.11845141871676429	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.6003470734578813E-4
10-11	0.0	0.0	0.0	0.0	3.6003470734578813E-4
12-13	0.0	0.0	0.0	1.8001735367289407E-4	3.6003470734578813E-4
14-15	0.0	0.0	0.0	3.6003470734578813E-4	3.6003470734578813E-4
16-17	0.0	0.0	0.0	0.0010801041220373643	3.6003470734578813E-4
18-19	0.0	0.0	0.0	0.0014401388293831525	3.6003470734578813E-4
20-21	0.0	0.0	0.0	0.0016201561830560466	3.6003470734578813E-4
22-23	0.0	0.0	0.0	0.002880277658766305	3.6003470734578813E-4
24-25	0.0	0.0	0.0	0.008100780915280232	3.6003470734578813E-4
26-27	0.0	0.0	0.0	0.015301475062195997	3.6003470734578813E-4
28-29	0.0	0.0	0.0	0.026642568343588323	3.6003470734578813E-4
30-31	0.0	0.0	0.0	0.05202501521146638	3.6003470734578813E-4
32-33	0.0	0.0	0.0	0.09324898920255913	3.6003470734578813E-4
34-35	0.0	0.0	0.0	0.13195272024223134	3.6003470734578813E-4
36-37	0.0	0.0	0.0	0.1850578395757351	3.6003470734578813E-4
38-39	0.0	0.0	0.0	0.2592249892889674	3.6003470734578813E-4
40-41	0.0	0.0	0.0	0.3458133364056295	3.6003470734578813E-4
42-43	0.0	0.0	0.0	0.4185403472894787	3.6003470734578813E-4
44-45	0.0	0.0	0.0	0.4943276531857671	3.6003470734578813E-4
46-47	0.0	0.0	0.0	0.5899168679860738	3.6003470734578813E-4
48-49	0.0	0.0	0.0	0.6802855795298667	3.6003470734578813E-4
50-51	0.0	0.0	0.0	0.7668739266465288	3.6003470734578813E-4
52-53	0.0	0.0	0.0	0.8531022390558449	3.6003470734578813E-4
54-55	0.0	0.0	0.0	0.9440110026606565	3.6003470734578813E-4
56-57	0.0	0.0	0.0	1.0414003909976923	3.6003470734578813E-4
58-59	0.0	0.0	0.0	1.1526511155675407	3.6003470734578813E-4
60-61	0.0	0.0	0.0	1.255981076575782	3.6003470734578813E-4
62-63	0.0	0.0	0.0	1.354450569034855	3.6003470734578813E-4
64-65	0.0	0.0	0.0	1.4558003391526944	3.6003470734578813E-4
66-67	0.0	0.0	0.0	1.5641707860637766	3.6003470734578813E-4
68-69	0.0	0.0	0.0	1.6950434021839706	3.6003470734578813E-4
70-71	0.0	0.0	0.0	1.8394173198296315	3.6003470734578813E-4
72-73	0.0	0.0	0.0	1.9558885476559942	3.6003470734578813E-4
74-75	0.0	0.0	0.0	2.0723597754823566	3.6003470734578813E-4
76-77	0.0	0.0	0.0	2.201072183358476	3.6003470734578813E-4
78-79	0.0	0.0	0.0	2.3546269860414544	3.6003470734578813E-4
80-81	0.0	0.0	0.0	2.519882916713171	3.6003470734578813E-4
82-83	0.0	0.0	0.0	2.688739194458346	3.6003470734578813E-4
84-85	0.0	0.0	0.0	2.8503947780566046	3.6003470734578813E-4
86-87	0.0	0.0	0.0	3.0122303790085363	3.6003470734578813E-4
88-89	0.0	0.0	0.0	3.2021486871334397	3.6003470734578813E-4
90-91	0.0	0.0	0.0	3.393687151441399	3.6003470734578813E-4
92-93	0.0	0.0	0.0	3.5722643662849096	3.6003470734578813E-4
94-95	0.0	0.0	0.0	3.751741667896785	3.6003470734578813E-4
96-97	0.0	0.0	0.0	3.945800375156165	3.6003470734578813E-4
98-99	0.0	0.0	0.0	4.147959863330825	3.6003470734578813E-4
100-101	0.0	0.0	0.0	4.367761052165429	3.6003470734578813E-4
102-103	0.0	0.0	0.0	4.612224618453219	3.6003470734578813E-4
104-105	0.0	0.0	0.0	4.855248045911626	3.6003470734578813E-4
106-107	0.0	0.0	0.0	5.090710744515771	3.6003470734578813E-4
108-109	0.0	0.0	0.0	5.330313842254393	3.6003470734578813E-4
110-111	0.0	0.0	0.0	5.5864785365309215	5.400520610186821E-4
112-113	0.0	0.0	0.0	5.87810664948101	7.200694146915763E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACC	70	0.0027768682	34.021175	4
ACGCAAG	70	0.0027842424	34.00278	8
TATATAC	85	0.007179236	28.017437	2
AGGGCTA	155	6.774044E-6	26.887703	5
GTACTAG	180	7.149538E-7	26.48957	1
AGTACTC	140	1.04864754E-4	25.51588	5
CATGGGT	290	1.4370016E-10	24.636023	4
CTAGTAC	145	1.3308608E-4	24.636023	3
CATGGGG	1105	0.0	23.168264	4
GTATTTA	155	2.0765411E-4	23.07156	1
AATAGTG	135	0.0025492345	22.03884	7
GACGCTA	70	0.0031521334	21.25174	12-13
ATGGGGG	725	0.0	20.530018	5
GTATCAA	2260	0.0	20.042994	1
CTATTGA	155	0.0056748455	19.195118	9
AACGCAG	2410	0.0	19.011929	6
GTACTGT	190	8.295425E-4	18.791012	6
ATCAACG	2360	0.0	18.668398	3
CCTAGTA	160	0.00679545	18.60533	2
ACGCAGA	2490	0.0	18.401104	7
>>END_MODULE