FastQCFastQC Report
Sun 1 Jan 2017
ERR1142834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277751
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.5648944558255417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.5317712627497291No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5040.18145749250227722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.16849624303782884No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4410.15877530593949257No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3280.1180913840094185No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3130.11269086339923168No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.10296992630089541No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2810.10116975276416647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG753.6379788E-1271.451021
CTAGTGC505.325737E-447.5997124
GCGCAGA1304.1569365E-836.641551
GAGGTAC700.002785447533.9997946
GGTATCA19350.031.6944661
AGTACCT1203.686193E-529.749825
GTAACAC800.005355448529.749823
GTATCAA26050.029.4855441
TTGTACC850.00720131727.9998324
GGGCGAT850.00720131727.9998327
ATCAACG28900.026.9704273
CTAGTAC2006.0812454E-826.7748373
TATCAAC28750.026.697232
CAACGCA29450.026.062665
TGTTATC1150.00100292625.8694082
ATACGGG1150.00100292625.8694083
ACTGTGC3007.2759576E-1225.7831768
GTGATAC1401.0531429E-425.4998469
TCAACGC30950.025.376264
CATGGGG10100.025.3315284
Produced by FastQC (version 0.11.5)