FastQCFastQC Report
Sun 1 Jan 2017
ERR1142774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.45362903225806456No Hit
GTCCTTGATCTCCCAGGCATAGCTCCCATCCCGGAGCATGAAGATGGCGC50.25201612903225806No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30.15120967741935484No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG20.10080645161290322No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10080645161290322No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20.10080645161290322No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC20.10080645161290322No Hit
AGAGTGACTTCAGCACACCGGTCTTGACTGACCAAAAAGTCCTTGATCTC20.10080645161290322No Hit
CATGAAGATGGCGCGGTCGGATCCCACGATGAACCTCTGAACGTCATAGT20.10080645161290322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10080645161290322No Hit
GACCAGATGGTCATACACAGACCATTTCTAGAGGACATGAGAATGTCATC20.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)