FastQCFastQC Report
Sun 1 Jan 2017
ERR1142774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCATCTGAAAA412.066532258064516No Hit
GGGTTTATTGTACACGAGCTACACTTGGCCACAAGAGAACAGAGCTAGTC170.8568548387096774No Hit
ATTTCTTAAAGAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAA160.8064516129032258No Hit
CCTTAATGGGCAGGGAGAACAGGTAGATCTCCTCCAAGGACTTGATTTTC140.7056451612903225No Hit
AAATTAAATCTTGCTTAAGGGAACATTGTAAAGTAACAATTCTTGATATC90.45362903225806456No Hit
TTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCATCTGAA90.45362903225806456No Hit
GCTTCTGCACTGGCATGATTTTCAGAACCTCATCCTTTAGGGACGCACCC80.4032258064516129No Hit
GTGTGTCACTGTTCCAAGAGACAAAACAAATTTAAACAGCTAAACCATCT70.35282258064516125No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC70.35282258064516125No Hit
TTCTGGAGCAGTGGGAGAAAGATGATGACATAGAAGAAGGAGACCTTCCA60.3024193548387097No Hit
CTTCTTGGCCGCCTGGCGGCCATTGTGGCCAAGCAGGTACTTCTGGGCCG60.3024193548387097No Hit
GTTTTGGTTGTAAGAAAGGCGGTCATTTCTCCAGGCGATCGCGGCGAGGT60.3024193548387097No Hit
GCAGCACACAGTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCT50.25201612903225806No Hit
GGTCACAAGCCTCGTGCTCTGCATTGTGAGCCATGTTGTAGGGGACGATT50.25201612903225806No Hit
GTGCCCAGAAGGTACGGGTTGATCTCTATTACTTTCTTCACCGTCTGGGA50.25201612903225806No Hit
CAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTA40.20161290322580644No Hit
GACCAACGTGACCATTGTAGTCCCCAATAGCGACGAAAGCCTTGAACCTG40.20161290322580644No Hit
GAACATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCAT40.20161290322580644No Hit
CCTTAATGGGCAGGGAGAACAGGTAGATCTCCTCCAAGGACTTGATCTTC40.20161290322580644No Hit
CCAGCCCGAGTCTGCTTCTGCACTGGCATGATTTTCAGAACCTCATCCTT40.20161290322580644No Hit
GTGTTCTGAGCTGGCACAGTGACCTCACACGGGGCGATGGCACCAGCCCG30.15120967741935484No Hit
GAAGGAGTGGTAACTGGTTCAGGAACCCTTGCCAGCCTCAGGAAATCCTG30.15120967741935484No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.15120967741935484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.15120967741935484No Hit
ACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTCCC30.15120967741935484No Hit
CTATTTCAAAAGTACTTTTGTCCATCTGTTCTCTGTTGTGTGATCCCAAT30.15120967741935484No Hit
GTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCTGCTCCTGCAG30.15120967741935484No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.15120967741935484No Hit
GTGGGAGAAAGATGATGACATAGAAGAAGGAGACCTTCCAGAACACAAGA30.15120967741935484No Hit
CCTATCGACTTCTCAAAGCTAGACCCAGGCAAACCTGAGAGCATCTTGAA30.15120967741935484No Hit
GCTTAAGGGAACATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTA20.10080645161290322No Hit
ACGCAGAGTACATGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCAT20.10080645161290322No Hit
GGTTACTTCCCCAGCTTGTCTCATGAAATCTTTGAGATCCTGCCAGCTGA20.10080645161290322No Hit
GCTGGGAACAGGACTTCTAACAGCAAGTATGTCTGGAAGGCTGTGGTGCA20.10080645161290322No Hit
GTTTCTCCAGAGCTGGGTTGTTCTCCAGGTGGCCCCTGATAGCCTTGCGC20.10080645161290322No Hit
GTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGAACCCTCCAGCTCG20.10080645161290322No Hit
GAGTATACTGTCTAAAAAGTCAAAACAAAGTCAAATTTATGTCTAGAATC20.10080645161290322No Hit
CTGTTTGTGGCCATGATTACCTTCACATTGACGTTCTGGTCAAATCCATC20.10080645161290322No Hit
ACCTCACACGGGGCGATGGCACCAGCCCGAGCAGCAGCTGGCACCTTATT20.10080645161290322No Hit
GAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGC20.10080645161290322No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.10080645161290322No Hit
GTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTG20.10080645161290322No Hit
CCCACTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGC20.10080645161290322No Hit
GTGCCAGATGCCGACGGCTCGTCTCTTCATCTTGGTCTTGCCACAGAAGG20.10080645161290322No Hit
CTGATGATGTTTGTCACCGTGTCTGGGAACCCCACCGAGAAGGAGACAGA20.10080645161290322No Hit
GTCTTACAGTCACCATTCGGGACCTTCCACATCCAAGTGTTGCCATCGGC20.10080645161290322No Hit
GGGTGTTCTTCTCCTGCGTCTCGGTTTTCTTCAGCTTGGCCTTATCGAAG20.10080645161290322No Hit
GCCTCAGGAAATCCTGGAAGTCTGCTGTTGGTTAATACCCTGCACAGATC20.10080645161290322No Hit
CCTGAGAGCATCTTGAAAATGACAAAGAAAGGGAAGACTCTGATGATGTT20.10080645161290322No Hit
CAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTGTGTACTGCGGACA20.10080645161290322No Hit
GTGTCTCATCTGCCGGGCAATGTTGACAATCTCATCAAAAGTAATGTTCC20.10080645161290322No Hit
ATCTTGCTTAAGGGAACATTGTAAAGTAACAATTCTTGATATCACGTGCC20.10080645161290322No Hit
GTTCCATATGAATAACCTGACATAAGTTAGCATCCATTAGTTCCATTACC20.10080645161290322No Hit
CTGCCAGTGTTCTCTGGAGACCCTAAGGTGGTTCCTAGAACTGCCAACCA20.10080645161290322No Hit
CTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTCCCG20.10080645161290322No Hit
GTGTGGGGCTTGCCAATCTTGTTCCCCCAGTAGCCTCTCCGCACAGGGAC20.10080645161290322No Hit
GGTTCCACTCCTGGAGCCTGGTCTGCTTCCCCAGTGAACGTAATCCTCTG20.10080645161290322No Hit
GGATCACTTGGCCCTTCCTCTTCTTGTCGCCTCCCAGTTCAAAATGCTTG20.10080645161290322No Hit
GGTCTAGGGGCTTGTATTTCTTGCCCTTGTAGAATTTCCTGAGGTTTTCT20.10080645161290322No Hit
GATTTGGAATTATTTCACATGTAGTTACTTACTACTCCGGAAAAAAAGAA20.10080645161290322No Hit
ACACAAGAGACCCTCAGCACCTATCGACTTCTCAAAGCTAGACCCAGGCA20.10080645161290322No Hit
AAATTAGACCTTTCCAACCCTTAACATCTGCATTTAATCATATTTCCTAA20.10080645161290322No Hit
GGTAGATCTCCTCCAAGGACTTGATTTTCATGTCCTTAACCAGGCGGCCC20.10080645161290322No Hit
GCACACAGTCCGATAGCTTGTCCCGGCCCTCAGCATCCCTCACTTCTGCT20.10080645161290322No Hit
ACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCATCCGGGGAAATTGTG20.10080645161290322No Hit
CAGTAAGACAAATCTATGAGAACACAGAATAGCACAGCACATAGCTAACA20.10080645161290322No Hit
CATTTGTGCGATGCCTCGGTTGGTGAGGATGTAGCGGGCGTGGATCAACC20.10080645161290322No Hit
CATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCATCTG20.10080645161290322No Hit
GCTCCTGTCTGGGCATCGAATCTCTTGGTGGCAATGGCATCAATTTCATC20.10080645161290322No Hit
GAAGTCAACATAATAGGTCACAACGATGGATGCCAAAATCCAGAATCCAG20.10080645161290322No Hit
GCCTCCATGAAGGCTTTGGTGCCCGACTTGGCAATGGTGCCAAGGTTATT20.10080645161290322No Hit
CTTAACCAGGCGGCCCAGCTTGGTGACGGGGATCCACTCCTTGTCTTCAG20.10080645161290322No Hit
AGGGAACATTGTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATC20.10080645161290322No Hit
GGCCCCTCGGATGGCAGTGGCAACCTCCTTGGAGCACTTAACACCAAGAC20.10080645161290322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTTT207.258981E-489.249991
ACTTTTT300.003630799559.53
TACTTTT350.006686063551.02
Produced by FastQC (version 0.11.5)