FastQCFastQC Report
Sun 1 Jan 2017
ERR1142769_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142769_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.6219458018658374No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC40.1776988005330964No Hit
GAAGGCAGGGAAATGTTAAATTCAGGAAGATAGTAGTTAAATGTGCATAA30.13327410039982232No Hit
GGGCTCATTGGCTCTTATTAGCCCCACTTGAGTGTGGAAGAGTCACTCAA30.13327410039982232No Hit
GAACTAGTACTGTATAAATGTGTATTATATGTTAACATTATCATTTGTAA30.13327410039982232No Hit
AGTGGGGAAAATGGAGAGTAAAGAAGAGGAAACTGCAGTAAAATATACAA30.13327410039982232No Hit
GTGGACTCGAATGGAATCATCATCAAATGAAATAGAATGCAATCATCAAA30.13327410039982232No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)