##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142769_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2251
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.257219013771657	27.0	14.0	33.0	14.0	33.0
2	23.645490892936472	27.0	14.0	33.0	14.0	33.0
3	24.080408707241226	27.0	14.0	33.0	14.0	33.0
4	24.033762772101287	27.0	14.0	33.0	14.0	33.0
5	23.928476232785428	27.0	14.0	33.0	14.0	33.0
6	25.776099511328297	27.0	14.0	37.0	14.0	37.0
7	25.629942247889826	27.0	14.0	37.0	14.0	37.0
8	25.84406930253221	27.0	14.0	37.0	14.0	37.0
9	25.192358951577077	27.0	14.0	37.0	14.0	37.0
10-11	25.478231896934695	27.0	14.0	37.0	14.0	37.0
12-13	25.749222567747665	27.0	14.0	37.0	14.0	37.0
14-15	25.46090626388272	27.0	14.0	37.0	14.0	37.0
16-17	25.537316748111948	27.0	14.0	37.0	14.0	37.0
18-19	25.314971123944915	27.0	14.0	37.0	14.0	37.0
20-21	25.469791203909374	27.0	14.0	37.0	14.0	37.0
22-23	25.404931141714794	27.0	14.0	37.0	14.0	37.0
24-25	24.91426032874278	27.0	14.0	37.0	14.0	37.0
26-27	25.196579298089738	27.0	14.0	37.0	14.0	37.0
28-29	24.806308307418924	27.0	14.0	37.0	14.0	37.0
30-31	24.926699244780096	27.0	14.0	37.0	14.0	37.0
32-33	25.005108840515327	27.0	14.0	37.0	14.0	37.0
34-35	24.803198578409596	27.0	14.0	37.0	14.0	37.0
36-37	24.905375388716127	27.0	14.0	37.0	14.0	37.0
38-39	24.709684584629052	27.0	14.0	37.0	14.0	37.0
40-41	24.74855619724567	27.0	14.0	37.0	14.0	37.0
42-43	24.745224344735675	27.0	14.0	37.0	14.0	37.0
44-45	24.863394047090182	27.0	14.0	37.0	14.0	37.0
46-47	24.843847179031542	27.0	14.0	37.0	14.0	37.0
48-49	24.729231452687692	27.0	14.0	37.0	14.0	37.0
50-51	24.83784984451355	27.0	14.0	37.0	14.0	37.0
52-53	24.804975566414925	27.0	14.0	37.0	14.0	37.0
54-55	24.762105730786317	27.0	14.0	37.0	14.0	37.0
56-57	24.638605064415813	27.0	14.0	37.0	14.0	37.0
58-59	24.548645046645937	27.0	14.0	37.0	14.0	37.0
60-61	24.89338071968014	27.0	14.0	37.0	14.0	37.0
62-63	24.414482452243448	27.0	14.0	37.0	14.0	37.0
64-65	24.462461128387382	27.0	14.0	37.0	14.0	37.0
66-67	24.438471790315415	27.0	14.0	37.0	14.0	37.0
68-69	24.429808973789427	27.0	14.0	37.0	14.0	37.0
70-71	24.434695690804087	27.0	14.0	37.0	14.0	37.0
72-73	24.3758329631275	27.0	14.0	37.0	14.0	37.0
74-75	24.13638382940915	24.5	14.0	37.0	14.0	37.0
76-77	24.185250999555755	27.0	14.0	37.0	14.0	37.0
78-79	24.15104398045313	27.0	14.0	37.0	14.0	37.0
80-81	24.228120835184363	27.0	14.0	37.0	14.0	37.0
82-83	24.094402487783206	24.5	14.0	37.0	14.0	37.0
84-85	23.90515326521546	22.0	14.0	37.0	14.0	37.0
86-87	23.719680142159042	22.0	14.0	37.0	14.0	37.0
88-89	23.662372278987117	22.0	14.0	37.0	14.0	37.0
90-91	23.533318525099954	22.0	14.0	37.0	14.0	37.0
92-93	23.463349622390048	22.0	14.0	37.0	14.0	37.0
94-95	23.307418924922256	22.0	14.0	35.0	14.0	37.0
96-97	23.36294980008885	22.0	14.0	35.0	14.0	37.0
98-99	23.229675699689025	22.0	14.0	33.0	14.0	37.0
100-101	23.04220346512661	22.0	14.0	33.0	14.0	37.0
102-103	22.845402043536208	22.0	14.0	33.0	14.0	37.0
104-105	22.936472678809416	22.0	14.0	33.0	14.0	37.0
106-107	22.751221679253664	22.0	14.0	33.0	14.0	37.0
108-109	22.47045757441137	22.0	14.0	33.0	14.0	37.0
110-111	22.446023989338073	22.0	14.0	33.0	14.0	37.0
112-113	22.145935139937805	18.0	14.0	33.0	14.0	37.0
114-115	22.33851621501555	18.0	14.0	33.0	14.0	37.0
116-117	22.264993336294978	18.0	14.0	33.0	14.0	37.0
118-119	22.163927143491783	18.0	14.0	33.0	14.0	37.0
120-121	21.917814304753442	14.0	14.0	33.0	14.0	37.0
122-123	21.67125721901377	14.0	14.0	30.0	14.0	37.0
124-125	21.004442470013327	14.0	14.0	27.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
0	1.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	9.0
15	104.0
16	177.0
17	236.0
18	227.0
19	190.0
20	151.0
21	116.0
22	80.0
23	80.0
24	48.0
25	59.0
26	46.0
27	49.0
28	37.0
29	40.0
30	43.0
31	38.0
32	48.0
33	70.0
34	79.0
35	105.0
36	217.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.79402585822559	27.819884083816316	15.470352206865803	22.91573785109229
2	20.14260249554367	28.25311942959002	28.698752228163993	22.905525846702318
3	18.003565062388592	31.951871657754012	25.35650623885918	24.688057040998217
4	16.577540106951872	25.757575757575758	28.520499108734406	29.144385026737968
5	17.46880570409982	37.29946524064171	25.935828877005346	19.29590017825312
6	24.442462087421944	36.663693131132916	20.115967885816236	18.777876895628903
7	22.757697456492636	35.475234270414994	20.39268183846497	21.3743864346274
8	23.50579839429081	33.58608385370205	20.829616413916145	22.078501338090987
9	23.293172690763054	27.755466309683175	19.27710843373494	29.674252565818833
10-11	21.5305667112896	32.1508255243195	23.22623828647925	23.09236947791165
12-13	21.9892952720785	30.709188224799288	23.840321141837645	23.461195361284567
14-15	21.128960285586793	30.410531012940652	22.311468094600624	26.14904060687193
16-17	22.115812917594653	32.761692650334076	21.469933184855233	23.652561247216035
18-19	21.908362989323845	30.894128113879006	23.22064056939502	23.976868327402133
20-21	22.67674522009782	32.19208537127612	21.787461093819473	23.34370831480658
22-23	23.49933303690529	29.879946642952422	22.23210315695865	24.38861716318364
24-25	22.098710538016896	31.325033348154737	21.765228990662518	24.81102712316585
26-27	22.054246331702977	30.880391285015563	22.49888839484215	24.566473988439306
28-29	22.67674522009782	30.168963983992885	22.69897732325478	24.455313472654513
30-31	22.78284063125139	29.91775950211158	23.449655478995332	23.849744387641696
32-33	21.943085815918188	30.013339261894174	22.321031569586484	25.722543352601157
34-35	22.560569015336743	29.317626139142032	23.094020893531898	25.02778395198933
36-37	21.76039119804401	28.917537230495665	22.82729495443432	26.494776617026005
38-39	22.459417389370692	28.619079386257507	23.415610406937958	25.505892817433846
40-41	22.23210315695865	28.968430413517122	22.67674522009782	26.122721209426413
42-43	22.187638950644732	28.479324144064027	24.0329035126723	25.30013339261894
44-45	21.16025783507446	28.71749277617248	24.00533451878195	26.116914869971104
46-47	23.427428317403866	27.250500111135807	22.538341853745276	26.783729717715048
48-49	21.226939319848857	29.25094465436764	24.51655923538564	25.005556790397865
50-51	21.871527006001333	28.406312513891972	24.116470326739275	25.605690153367416
52-53	22.755555555555556	27.71111111111111	23.91111111111111	25.622222222222224
54-55	22.133333333333333	29.42222222222222	22.666666666666664	25.77777777777778
56-57	22.555555555555557	28.71111111111111	22.77777777777778	25.955555555555556
58-59	22.893976439208714	27.739497666148033	22.78284063125139	26.583685263391864
60-61	21.777777777777775	29.06666666666667	23.333333333333332	25.822222222222223
62-63	22.88888888888889	28.000000000000004	23.466666666666665	25.644444444444442
64-65	23.227383863080682	29.07312736163592	21.738164036452545	25.96132473883085
66-67	23.182929539897756	28.406312513891972	22.471660368970884	25.939097577239384
68-69	24.066666666666666	28.42222222222222	21.688888888888886	25.822222222222223
70-71	23.400000000000002	28.77777777777778	22.26666666666667	25.555555555555554
72-73	22.955555555555556	28.97777777777778	22.555555555555557	25.511111111111113
74-75	24.299688750555802	28.701645175633615	22.632281013783903	24.36638506002668
76-77	24.627695043342964	28.295176705934654	22.338297399422093	24.73883085130029
78-79	23.21031569586483	29.07959092930191	22.321031569586484	25.389061805246776
80-81	22.916203600800177	29.495443431873753	22.538341853745276	25.050011113580794
82-83	24.18315181151367	28.006223605245612	22.893976439208714	24.91664814403201
84-85	24.2	29.06666666666667	22.77777777777778	23.955555555555556
86-87	23.044444444444444	30.288888888888888	21.88888888888889	24.77777777777778
88-89	24.555555555555557	29.577777777777776	21.91111111111111	23.955555555555556
90-91	22.53333333333333	30.355555555555558	22.26666666666667	24.844444444444445
92-93	23.11624805512336	29.806623694154254	21.560346743720828	25.516781507001557
94-95	22.644444444444446	29.622222222222224	22.11111111111111	25.622222222222224
96-97	23.755555555555556	29.644444444444446	22.42222222222222	24.177777777777777
98-99	22.733333333333334	30.88888888888889	22.066666666666666	24.31111111111111
100-101	23.48888888888889	30.488888888888887	21.488888888888887	24.53333333333333
102-103	22.382751722605025	31.14025338964214	21.871527006001333	24.6054678817515
104-105	21.133333333333333	30.866666666666664	22.377777777777776	25.622222222222224
106-107	22.444444444444443	32.4	19.955555555555556	25.2
108-109	22.538341853745276	31.45143365192265	21.76039119804401	24.249833296288063
110-111	21.88888888888889	32.84444444444444	20.4	24.866666666666667
112-113	21.977777777777778	31.666666666666664	22.62222222222222	23.733333333333334
114-115	21.688888888888886	33.24444444444444	20.466666666666665	24.6
116-117	21.58257390531229	33.474105356745945	20.071126917092688	24.872193820849077
118-119	20.822222222222223	35.06666666666667	19.933333333333334	24.177777777777777
120-121	21.46666666666667	33.955555555555556	19.466666666666665	25.11111111111111
122-123	21.133333333333333	33.24444444444444	20.711111111111112	24.91111111111111
124-125	21.133333333333333	35.333333333333336	19.11111111111111	24.422222222222224
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	6.0
2	3.5
3	1.5
4	0.0
5	1.5
6	2.5
7	1.5
8	2.0
9	3.5
10	2.5
11	1.5
12	1.5
13	0.5
14	2.0
15	2.0
16	0.5
17	3.0
18	3.5
19	3.5
20	4.0
21	4.5
22	4.5
23	3.0
24	5.5
25	7.5
26	8.5
27	12.5
28	16.5
29	16.0
30	13.5
31	14.5
32	23.0
33	30.5
34	34.5
35	39.0
36	38.5
37	44.0
38	56.5
39	51.0
40	46.0
41	52.0
42	53.5
43	62.0
44	72.0
45	76.5
46	75.5
47	81.5
48	87.0
49	86.0
50	83.5
51	78.0
52	73.0
53	74.0
54	84.5
55	91.5
56	84.0
57	72.5
58	66.5
59	64.0
60	57.5
61	45.0
62	35.5
63	29.5
64	26.5
65	21.0
66	16.0
67	14.0
68	11.5
69	11.0
70	11.0
71	6.0
72	4.0
73	4.5
74	4.5
75	4.5
76	2.5
77	1.5
78	1.5
79	2.0
80	2.0
81	1.5
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3553976010661928
2	0.3109729009329187
3	0.3109729009329187
4	0.3109729009329187
5	0.3109729009329187
6	0.3998223011994669
7	0.444247001332741
8	0.3998223011994669
9	0.444247001332741
10-11	0.444247001332741
12-13	0.3998223011994669
14-15	0.444247001332741
16-17	0.26654820079964464
18-19	0.13327410039982232
20-21	0.0888494002665482
22-23	0.0888494002665482
24-25	0.0888494002665482
26-27	0.0888494002665482
28-29	0.0888494002665482
30-31	0.06663705019991116
32-33	0.0888494002665482
34-35	0.06663705019991116
36-37	0.06663705019991116
38-39	0.11106175033318524
40-41	0.0888494002665482
42-43	0.0888494002665482
44-45	0.06663705019991116
46-47	0.06663705019991116
48-49	0.06663705019991116
50-51	0.06663705019991116
52-53	0.0444247001332741
54-55	0.0444247001332741
56-57	0.0444247001332741
58-59	0.06663705019991116
60-61	0.0444247001332741
62-63	0.0444247001332741
64-65	0.06663705019991116
66-67	0.06663705019991116
68-69	0.0444247001332741
70-71	0.0444247001332741
72-73	0.0444247001332741
74-75	0.0888494002665482
76-77	0.06663705019991116
78-79	0.0888494002665482
80-81	0.06663705019991116
82-83	0.06663705019991116
84-85	0.0444247001332741
86-87	0.0444247001332741
88-89	0.0444247001332741
90-91	0.0444247001332741
92-93	0.06663705019991116
94-95	0.0444247001332741
96-97	0.0444247001332741
98-99	0.0444247001332741
100-101	0.0444247001332741
102-103	0.06663705019991116
104-105	0.0444247001332741
106-107	0.0444247001332741
108-109	0.06663705019991116
110-111	0.0444247001332741
112-113	0.0444247001332741
114-115	0.0444247001332741
116-117	0.06663705019991116
118-119	0.0444247001332741
120-121	0.0444247001332741
122-123	0.0444247001332741
124-125	0.0444247001332741
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2251.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.64549089293646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.45313921747044	96.13505108840515
2	1.2283894449499546	2.3989338071968014
3	0.22747952684258416	0.6663705019991115
4	0.04549590536851684	0.1776988005330964
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.04549590536851684	0.6219458018658374
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.6219458018658374	No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	4	0.1776988005330964	No Hit
GAAGGCAGGGAAATGTTAAATTCAGGAAGATAGTAGTTAAATGTGCATAA	3	0.13327410039982232	No Hit
GGGCTCATTGGCTCTTATTAGCCCCACTTGAGTGTGGAAGAGTCACTCAA	3	0.13327410039982232	No Hit
GAACTAGTACTGTATAAATGTGTATTATATGTTAACATTATCATTTGTAA	3	0.13327410039982232	No Hit
AGTGGGGAAAATGGAGAGTAAAGAAGAGGAAACTGCAGTAAAATATACAA	3	0.13327410039982232	No Hit
GTGGACTCGAATGGAATCATCATCAAATGAAATAGAATGCAATCATCAAA	3	0.13327410039982232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0444247001332741	0.0
2	0.0	0.0	0.0	0.0444247001332741	0.0
3	0.0	0.0	0.0	0.0444247001332741	0.0
4	0.0	0.0	0.0	0.0444247001332741	0.0
5	0.0	0.0	0.0	0.0888494002665482	0.0
6	0.0	0.0	0.0	0.1332741003998223	0.0
7	0.0	0.0	0.0	0.1332741003998223	0.0
8	0.0	0.0	0.0	0.1332741003998223	0.0
9	0.0	0.0	0.0	0.1776988005330964	0.0
10-11	0.0	0.0	0.0	0.22212350066637052	0.0
12-13	0.0	0.0	0.0	0.22212350066637052	0.0
14-15	0.0	0.0	0.0	0.22212350066637052	0.0
16-17	0.0	0.0	0.0	0.22212350066637052	0.0
18-19	0.0	0.0	0.0	0.22212350066637052	0.0
20-21	0.0	0.0	0.0	0.2665482007996446	0.0
22-23	0.0	0.0	0.0	0.2665482007996446	0.0
24-25	0.0	0.0	0.0	0.3553976010661928	0.0
26-27	0.0	0.0	0.0	0.3776099511328298	0.0
28-29	0.0	0.0	0.0	0.44424700133274103	0.0
30-31	0.0	0.0	0.0	0.5330964015992892	0.0
32-33	0.0	0.0	0.0	0.7996446023989339	0.0
34-35	0.0	0.0	0.0	1.0217681030653043	0.0
36-37	0.0	0.0	0.0	1.243891603731675	0.0
38-39	0.0	0.0	0.0	1.4882274544646823	0.0
40-41	0.0	0.0	0.0	1.8658374055975124	0.0
42-43	0.0	0.0	0.0	2.176810306530431	0.0
44-45	0.0	0.0	0.0	2.665482007996446	0.0
46-47	0.0	0.0	0.0	3.2430031097290093	0.0
48-49	0.0	0.0	0.0	3.4429142603287426	0.0
50-51	0.0	0.0	0.0	3.7094624611283873	0.0
52-53	0.0	0.0	0.0	4.087072412261217	0.0
54-55	0.0	0.0	0.0	4.553531763660596	0.0
56-57	0.0	0.0	0.0	4.9977787649933365	0.0
58-59	0.0	0.0	0.0	5.3087516659262555	0.0
60-61	0.0	0.0	0.0	5.575299866725899	0.0
62-63	0.0	0.0	0.0	5.752998667258996	0.0
64-65	0.0	0.0	0.0	6.152820968458463	0.0
66-67	0.0	0.0	0.0	6.619280319857841	0.0
68-69	0.0	0.0	0.0	6.7969791203909375	0.0
70-71	0.0	0.0	0.0	6.908040870724122	0.0
72-73	0.0	0.0	0.0	7.085739671257219	0.0
74-75	0.0	0.0	0.0	7.285650821856953	0.0
76-77	0.0	0.0	0.0	7.574411372723234	0.0
78-79	0.0	0.0	0.0	7.996446023989337	0.0
80-81	0.0	0.0	0.0	8.151932474455798	0.0
82-83	0.0	0.0	0.0	8.196357174589071	0.0
84-85	0.0	0.0	0.0	8.329631274988895	0.0
86-87	0.0	0.0	0.0	8.685028876055087	0.0
88-89	0.0	0.0	0.0	8.929364726788094	0.0
90-91	0.0	0.0	0.0	9.195912927587738	0.0
92-93	0.0	0.0	0.0	9.351399378054198	0.0
94-95	0.0	0.0	0.0	9.840071079520214	0.0
96-97	0.0	0.0	0.0	10.106619280319858	0.0
98-99	0.0	0.0	0.0	10.39537983118614	0.0
100-101	0.0	0.0	0.0	10.61750333185251	0.0
102-103	0.0	0.0	0.0	10.77298978231897	0.0
104-105	0.0	0.0	0.0	11.039537983118613	0.0
106-107	0.0	0.0	0.0	11.195024433585072	0.0
108-109	0.0	0.0	0.0	11.394935584184807	0.0
110-111	0.0	0.0	0.0	11.661483784984451	0.0
112-113	0.0	0.0	0.0	11.928031985784095	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE