FastQCFastQC Report
Sun 1 Jan 2017
ERR1142769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT451.9991115059973346No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC321.4215904042647711No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG231.0217681030653043No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC90.3998223011994669No Hit
GTTTTGGTTGTAAGAAAGGCGGTCATTTCTCCAGGCGATCGCGGCGAGGT80.3553976010661928No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.3109729009329187No Hit
CCTTAATGGGCAGGGAGAACAGGTAGATCTCCTCCAAGGACTTGATTTTC70.3109729009329187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.26654820079964464No Hit
CTTCTTGGCCGCCTGGCGGCCATTGTGGCCAAGCAGGTACTTCTGGGCCG60.26654820079964464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.26654820079964464No Hit
GTAAAGTAACAATTCTTGATATCACGTGCCTCTTATGATCCATCTGAAAA60.26654820079964464No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG50.2221235006663705No Hit
ATTTCTTAAAGAATTGAGTACAAAAAACCTTACATAAATTAAAGAATGAA50.2221235006663705No Hit
GGGTTTATTGTACACGAGCTACACTTGGCCACAAGAGAACAGAGCTAGTC50.2221235006663705No Hit
GTGCTCACGCGCGGAGCCCACTGGGCGAACAGCAACGTTATAACGGCCAC40.1776988005330964No Hit
CTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCAACTGCCATGT30.13327410039982232No Hit
CAATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTA30.13327410039982232No Hit
GCATCAAATTCTGCTTGGACAATGGAGCCAAGTCCGTTGTCCTTATGAGC30.13327410039982232No Hit
CGTCAGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAA30.13327410039982232No Hit
GTTTCACGAAGAAGCCAATGAATCCCATGATAGCAAATCCTATAGCTGTA30.13327410039982232No Hit
GATCATAGCCTCTATCTACTTACTTCCTGATAGTGTCAAGTATTTGTATA30.13327410039982232No Hit
GTTATTCGATGATTCCATTCGATCTCATTCGATGATGATTCCATTCAACT30.13327410039982232No Hit
GTCCCGCTTCTGGTACTTTGTGTCACAGCTGAAGAAGATGAAGAAGTCAT30.13327410039982232No Hit
TCCCTACCTGGCACTGTGCACATTACCAGACCTAGATGCGAGGAACCTGG30.13327410039982232No Hit
CCCTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAA30.13327410039982232No Hit
GTGTGTCACTGTTCCAAGAGACAAAACAAATTTAAACAGCTAAACCATCT30.13327410039982232No Hit
GCTTCTGCACTGGCATGATTTTCAGAACCTCATCCTTTAGGGACGCACCC30.13327410039982232No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG30.13327410039982232RNA PCR Primer, Index 31 (96% over 29bp)
GAAGTCAACATAATAGGTCACAACGATGGATGCCAAAATCCAGAATCCAG30.13327410039982232No Hit
CCTCCGCAACCCCGGCCAGCAGACACTCTCTCCAAGCGCAGGTCTTGCCC30.13327410039982232No Hit
CTGTGACTAAAACCATAGCTCATATAGGAATAATCCTATTTACAAATGAT30.13327410039982232No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)