FastQCFastQC Report
Sun 1 Jan 2017
ERR1142735_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142735_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818689
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28740.3510490552578574No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16100.1966558729871783No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18950.062.876231
GTATCAA30650.045.0944631
ATCAACG40250.033.746963
CAACGCA41050.033.3795435
TATCAAC41350.033.1373672
TCAACGC41350.032.993294
AACGCAG43150.032.033146
GTGGTAT9300.031.3890951
TGGTATC9800.030.3955232
ACGCAGA50400.027.0722167
CGCAGAG51200.026.7655898
GCAGAGT54800.024.8985399
CAGAGTA53850.023.23552510-11
AGAGTAC52100.021.78593310-11
GAGTACA57550.021.27577812-13
CTAACGC1500.004670487319.858413
GTACTAG1500.004670487319.858411
AGTACAT56900.019.58160412-13
GTACATG66300.019.498981
GTAATAC2201.01115744E-418.9557533
Produced by FastQC (version 0.11.5)