FastQCFastQC Report
Sun 1 Jan 2017
ERR1142735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818689
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46450.5673705155437535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40650.49652554266638493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24040.29364019792619667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.16599710024197223No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.12239079797090227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29100.074.636761
GTATCAA46300.047.2723772
GTGGTAT10750.039.8544581
CTCAACG3900.038.1441761
TATCAAC60300.036.5929343
ATCAACG62100.035.4364934
TGGTATC12400.034.534362
CAACGCA64550.034.181556
TCAACGC65350.033.7631075
AACGCAG67500.032.687697
ACGCAGA73100.030.1022138
ATCGTAC800.005371967329.7379193
CGCAGAG76550.028.667869
GAGTACT33950.026.3639712-13
CAGAGTA80350.026.27583710-11
CCGCAGA6150.026.1241111
GCAGAGT81100.025.1528599
GTACTTT37450.023.90004714-15
GAGTACA53850.022.52986112-13
AGAGTAC77850.022.11587110-11
Produced by FastQC (version 0.11.5)