FastQCFastQC Report
Sun 1 Jan 2017
ERR1142734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378969
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.2905250825265391No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7160.18893365948138238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.047.0012861
GTATCAA19800.034.2923552
GTCTAGG1203.6574304E-529.7880951
TACCGAC800.005327734629.784157
TATCAAC24350.027.6399423
ATCAACG24500.026.4983024
CAACGCA24950.026.2660546
TCTAGGA2057.664676E-826.148472
AACGCAG25450.025.980677
TCAACGC26200.024.7855135
GTACAAA2651.3515091E-924.729741
ACGCAGA27050.024.003498
CGCAGAG28250.022.9869219
CTAACGC1300.002037567622.9078483
ACGCCTA1653.1821043E-421.6640686
TTACACC1653.1878104E-421.658334
TCTAACG1400.003133290221.2715742
CAGAGTA29650.021.09780710-11
GTACATG34550.020.174861
GCAGAGT29300.020.1298399
Produced by FastQC (version 0.11.5)