##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142734_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	378969
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267090448031368	33.0	33.0	33.0	27.0	33.0
2	31.38967567268035	33.0	33.0	33.0	27.0	33.0
3	31.433027503568894	33.0	33.0	33.0	27.0	33.0
4	31.375740495924468	33.0	33.0	33.0	27.0	33.0
5	31.432489200963666	33.0	33.0	33.0	27.0	33.0
6	34.73286997089472	37.0	37.0	37.0	27.0	37.0
7	34.73619741984173	37.0	37.0	37.0	27.0	37.0
8	34.70297570513683	37.0	37.0	37.0	27.0	37.0
9	34.61495003549103	37.0	37.0	37.0	27.0	37.0
10-11	34.68063086954342	37.0	37.0	37.0	27.0	37.0
12-13	34.64839736231724	37.0	37.0	37.0	27.0	37.0
14-15	34.63646762663964	37.0	37.0	37.0	27.0	37.0
16-17	34.686115487018725	37.0	37.0	37.0	27.0	37.0
18-19	34.663391992484875	37.0	37.0	37.0	27.0	37.0
20-21	34.68367597349651	37.0	37.0	37.0	27.0	37.0
22-23	34.762422520047814	37.0	37.0	37.0	27.0	37.0
24-25	34.83111547382504	37.0	37.0	37.0	27.0	37.0
26-27	34.903790283637974	37.0	37.0	37.0	27.0	37.0
28-29	34.95857708678019	37.0	37.0	37.0	27.0	37.0
30-31	34.992351617150746	37.0	37.0	37.0	27.0	37.0
32-33	34.98922471231156	37.0	37.0	37.0	27.0	37.0
34-35	34.9930904638638	37.0	37.0	37.0	27.0	37.0
36-37	34.97951547488053	37.0	37.0	37.0	27.0	37.0
38-39	34.95115563542137	37.0	37.0	37.0	27.0	37.0
40-41	34.92980560415232	37.0	37.0	37.0	27.0	37.0
42-43	34.925358010813554	37.0	37.0	37.0	27.0	37.0
44-45	34.94122342460729	37.0	37.0	37.0	27.0	37.0
46-47	34.949213524061335	37.0	37.0	37.0	27.0	37.0
48-49	34.93752919104201	37.0	37.0	37.0	27.0	37.0
50-51	34.93405529212152	37.0	37.0	37.0	27.0	37.0
52-53	34.9033971116371	37.0	37.0	37.0	27.0	37.0
54-55	34.90256327034665	37.0	37.0	37.0	27.0	37.0
56-57	34.88195340515979	37.0	37.0	37.0	27.0	37.0
58-59	34.84698748446443	37.0	37.0	37.0	27.0	37.0
60-61	34.83657766202513	37.0	37.0	37.0	27.0	37.0
62-63	34.78746018803649	37.0	37.0	37.0	27.0	37.0
64-65	34.76378410899044	37.0	37.0	37.0	27.0	37.0
66-67	34.72256305924759	37.0	37.0	37.0	27.0	37.0
68-69	34.67593391543899	37.0	37.0	37.0	27.0	37.0
70-71	34.637541593111834	37.0	37.0	37.0	27.0	37.0
72-73	34.5800764706348	37.0	37.0	37.0	27.0	37.0
74-75	34.532761254878366	37.0	37.0	37.0	27.0	37.0
76-77	34.47876211510704	37.0	37.0	37.0	27.0	37.0
78-79	34.41923086057171	37.0	37.0	37.0	27.0	37.0
80-81	34.36697460742171	37.0	37.0	37.0	27.0	37.0
82-83	34.33030933928633	37.0	37.0	37.0	27.0	37.0
84-85	34.283422918497294	37.0	37.0	37.0	27.0	37.0
86-87	34.19788294029327	37.0	37.0	37.0	27.0	37.0
88-89	34.14199974140365	37.0	37.0	37.0	27.0	37.0
90-91	34.06585499077761	37.0	37.0	37.0	27.0	37.0
92-93	34.02339373405212	37.0	37.0	37.0	27.0	37.0
94-95	33.965495858500304	37.0	37.0	37.0	27.0	37.0
96-97	33.87978304294019	37.0	37.0	37.0	22.0	37.0
98-99	33.80229385517021	37.0	35.0	37.0	22.0	37.0
100-101	33.72961244851162	37.0	33.0	37.0	22.0	37.0
102-103	33.65718831883346	37.0	33.0	37.0	22.0	37.0
104-105	33.48050104362098	37.0	33.0	37.0	22.0	37.0
106-107	33.402745343286654	37.0	33.0	37.0	22.0	37.0
108-109	33.31366945581301	37.0	33.0	37.0	22.0	37.0
110-111	33.20961477060129	37.0	33.0	37.0	18.0	37.0
112-113	33.11319527454752	37.0	33.0	37.0	14.0	37.0
114-115	32.99689684380517	37.0	33.0	37.0	14.0	37.0
116-117	32.88422140069504	37.0	33.0	37.0	14.0	37.0
118-119	32.750951924827625	37.0	33.0	37.0	14.0	37.0
120-121	32.61906250907066	37.0	33.0	37.0	14.0	37.0
122-123	32.50978444147147	37.0	33.0	37.0	14.0	37.0
124-125	30.95884491871367	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	667.0
1	34.0
2	23.0
3	14.0
4	19.0
5	8.0
6	9.0
7	16.0
8	8.0
9	5.0
10	15.0
11	12.0
12	7.0
13	11.0
14	24.0
15	138.0
16	196.0
17	352.0
18	698.0
19	1314.0
20	2056.0
21	2654.0
22	2932.0
23	3164.0
24	3595.0
25	3910.0
26	4746.0
27	5693.0
28	6907.0
29	8322.0
30	10163.0
31	12376.0
32	15563.0
33	20797.0
34	31143.0
35	58915.0
36	182463.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.74458010539382	20.661900741356355	12.772185770510374	23.821333382739446
2	17.683978789386227	22.97260824927763	36.2525269630931	23.09088599824303
3	19.818077430859635	27.572011016951393	28.530835028825575	24.079076523363398
4	14.769203086465488	20.3073234755549	33.485663177281246	31.437810260698374
5	15.237326487673844	37.573672440486426	31.05106456180281	16.13793651003692
6	28.267586652439718	36.20828887412063	18.530423339961395	16.99370113347825
7	25.494105684598516	34.155254043405044	21.282556427610604	19.068083844385836
8	25.210046206193482	34.43619176232276	20.203095417775483	20.150666613708278
9	26.007334736730613	19.03854046682819	19.963458712316797	34.9906660841244
10-11	25.303921386903642	27.751709930172886	24.767971147659058	22.176397535264414
12-13	25.980268184267775	26.944216771910963	24.590090131841155	22.485424911980108
14-15	24.308995078861344	26.936250269755718	23.35786620910801	25.39688844227492
16-17	23.6683407777472	28.620684180444123	24.189361544486548	23.52161349732213
18-19	23.82013255897022	27.654552911137863	25.68032385664581	22.84499067324611
20-21	26.090309200353946	26.055390736290203	25.025957380956477	22.828342682399374
22-23	26.528679689463175	25.750486040390946	24.599463041091905	23.121371229053974
24-25	25.05005567619466	26.673790398302998	24.82576392764475	23.45038999785759
26-27	24.172259004832732	26.789658428713892	25.338119334368482	23.6999632320849
28-29	23.859579059840364	26.711160362438047	25.561482758803088	23.867777818918505
30-31	24.54013610120047	26.399030946757613	25.39704999457817	23.66378295746375
32-33	24.252691606261745	26.79135141711863	24.988395113648536	23.967561862971092
34-35	24.32706917550353	26.71689101000123	25.199317118196475	23.756722696298766
36-37	24.34591841187602	26.86716573240608	25.041357395337027	23.745558460380874
38-39	24.143590150493267	26.858023221984183	25.148509614113042	23.849877013409507
40-41	23.904046374236067	26.69202757701492	25.431386704959262	23.97253934378975
42-43	24.060706158022725	26.781428475086383	25.1778221791432	23.98004318774769
44-45	24.1636144865065	26.873553795501724	25.048460246730752	23.914371471261024
46-47	24.112202668218057	26.754769869497956	25.063267707686006	24.069759754597982
48-49	24.04977091363477	26.876822277905234	25.25635855156592	23.817048256894076
50-51	23.85383579867181	27.1518265896871	25.12489784691716	23.86943976472393
52-53	23.654851868747976	26.972780345253916	25.004925587268843	24.367442198729265
54-55	23.478658709935907	26.747521014681517	25.12413108242304	24.64968919295954
56-57	23.83157427070083	26.636906729571557	24.88846634949767	24.643052650229944
58-59	23.803775081815477	26.893524181018808	25.249942150672705	24.05275858649301
60-61	23.985618017941775	26.673049255247122	25.358491663845633	23.98284106296547
62-63	23.729252837693455	26.794649374279338	25.08952089790651	24.3865768901207
64-65	23.4543184931688	26.632030212790713	25.326749850575748	24.58690144346474
66-67	24.09128482531957	26.381076452033142	25.07833010402132	24.449308618625967
68-69	23.81155172778636	26.360115961656472	25.137545760416447	24.69078655014072
70-71	24.002713829819594	26.404030010831512	25.090229551384883	24.503026607964006
72-73	24.228668774956585	26.570008054884315	25.214829981099456	23.98649318905964
74-75	24.20356891071639	26.372004248514276	25.418601667396356	24.00582517337298
76-77	23.961946983413636	26.261765923425777	25.336627417164063	24.439659675996523
78-79	23.810934561392834	26.42951046028425	25.342746575179408	24.41680840314351
80-81	23.658402713944675	26.610015937150756	25.45487008907611	24.27671125982846
82-83	23.989277500869516	26.29154670859744	25.675941954621134	24.04323383591191
84-85	23.973828172005188	26.65223548826883	25.175574194725698	24.198362145000285
86-87	23.550535413727587	27.003124293464104	25.338376980449034	24.10796331235927
88-89	23.965953306106908	26.262968138735783	25.328244133516137	24.442834421641173
90-91	24.15327293593751	25.93795780644274	25.53006383507597	24.378705422543778
92-93	23.957037411139126	26.33825106196761	25.576992486570887	24.12771904032238
94-95	23.27687977653011	26.55873025105991	25.6956002712732	24.468789701136785
96-97	23.26558613050501	26.54341222780614	25.52416402084231	24.66683762084654
98-99	23.044647318702065	27.060843770077014	25.341391900716758	24.553117010504163
100-101	23.518781924512737	27.083619750631883	25.12346788777377	24.27413043708161
102-103	23.608175329920115	26.971159502479274	25.475266071006025	23.94539909659458
104-105	23.564073173601553	27.105882788444628	25.43862780327158	23.891416234682236
106-107	23.514038014610428	26.942438720014383	25.397847301724113	24.145675963651072
108-109	23.316155337033344	27.25114160080593	25.25667839776624	24.176024664394482
110-111	23.061080155724323	27.90955298658894	25.153972754853164	23.875394102833575
112-113	23.03943211410594	27.916562016682313	24.82266784755912	24.221338021652635
114-115	23.160345121594954	28.0415922547248	24.863084230132177	23.93497839354807
116-117	23.14484082394528	27.758976704589017	25.020820719722707	24.075361751742992
118-119	23.056790032068484	27.54095803602351	25.20495541344021	24.197296518467798
120-121	23.13696044164071	27.496787634906116	25.423291894264278	23.942960029188896
122-123	23.08649155970337	27.83645520760354	25.43232560906291	23.64472762363019
124-125	23.27964957697764	27.676332034539886	25.032690194792572	24.0113281936899
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1340.0
1	823.5
2	242.0
3	153.5
4	120.5
5	110.0
6	97.0
7	84.0
8	78.0
9	82.0
10	94.0
11	84.5
12	73.5
13	78.0
14	90.0
15	97.5
16	102.0
17	121.5
18	145.0
19	163.5
20	202.5
21	247.5
22	297.5
23	378.0
24	474.5
25	575.0
26	744.5
27	936.5
28	1196.0
29	1731.5
30	2446.0
31	3109.0
32	3920.5
33	4775.5
34	5416.0
35	6141.5
36	6984.0
37	8034.0
38	8630.5
39	8728.5
40	8857.5
41	8825.5
42	9225.0
43	10101.0
44	11307.5
45	12623.5
46	14208.0
47	15838.5
48	16636.0
49	16712.5
50	16654.5
51	16683.0
52	16434.0
53	16230.0
54	15948.5
55	15314.5
56	14521.5
57	12780.5
58	10966.5
59	9614.5
60	8176.0
61	6839.5
62	5494.0
63	4367.0
64	3390.5
65	2543.0
66	1944.5
67	1517.5
68	1224.0
69	1024.0
70	851.0
71	718.5
72	635.5
73	545.0
74	434.5
75	372.0
76	283.5
77	196.0
78	152.5
79	107.0
80	70.5
81	53.0
82	32.0
83	17.5
84	9.5
85	3.5
86	2.0
87	1.0
88	1.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30292715235283096
2	0.27574814826542543
3	0.26545706904786404
4	0.27997012948288647
5	0.29395544226572623
6	0.33828624504906735
7	0.3475218289622634
8	0.34673020748398947
9	0.34673020748398947
10-11	0.34884119809272
12-13	0.3576809712667791
14-15	0.34633439674485245
16-17	0.2789146341785212
18-19	0.2702067979175078
20-21	0.24949270256933945
22-23	0.24118067704746296
24-25	0.2348477052212714
26-27	0.24329166765619353
28-29	0.22772311191680583
30-31	0.22904248104726244
32-33	0.23629901126477362
34-35	0.22838279648203416
36-37	0.22759117500376022
38-39	0.2319450931342669
40-41	0.21848752800360977
42-43	0.22495243674284707
44-45	0.21743203269924452
46-47	0.21479329443833137
48-49	0.220730455525386
50-51	0.2269314904385319
52-53	0.22204982465584255
54-55	0.22178595082975125
56-57	0.21954302330797507
58-59	0.2194110863949294
60-61	0.22613986896025795
62-63	0.2229733830471622
64-65	0.2245566260037101
66-67	0.24315973074314784
68-69	0.24091680322137166
70-71	0.23893774952568678
72-73	0.2473817119606089
74-75	0.2518675670041613
76-77	0.2421042354387826
78-79	0.24276392000401087
80-81	0.24315973074314784
82-83	0.23366027300386047
84-85	0.22495243674284707
86-87	0.21202261926437255
88-89	0.20648126891645494
90-91	0.21294617765569215
92-93	0.20568964743818097
94-95	0.19856505413371542
96-97	0.18471167826392132
98-99	0.19473888365539133
100-101	0.19341951452493475
102-103	0.19355145143798042
104-105	0.20384253065554173
106-107	0.1968498742641219
108-109	0.2037105937424961
110-111	0.19434307291625436
112-113	0.1910446500901129
114-115	0.19144046082924987
116-117	0.19526663130757396
118-119	0.18893365948138238
120-121	0.19605825278584793
122-123	0.1910446500901129
124-125	0.21043937630782464
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	378969.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.31341149775471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.87893035826475	61.95632911123708
2	8.739504230740296	12.464877229863022
3	1.9657853506608907	4.205605788838144
4	0.7318027664085884	2.0874940726436377
5	0.3540238563210697	1.2623324472923219
6	0.22920837757044488	0.9807378809048319
7	0.16362732933897928	0.8168176148600483
8	0.10997405185155379	0.6274099851012215
9	0.08102085776908509	0.5200086392989041
>10	0.6616509396787548	9.372606447779077
>50	0.06523571038478111	3.155431268297316
>100	0.01839981574949493	2.008489707204353
>500	4.1817763067033935E-4	0.21352318193887404
>1k	4.1817763067033935E-4	0.32833662474120157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1101	0.2905250825265391	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	716	0.18893365948138238	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.638738260913162E-4	0.0
3	0.0	0.0	0.0	2.638738260913162E-4	0.0
4	0.0	0.0	0.0	2.638738260913162E-4	0.0
5	0.0	0.0	0.0	5.277476521826324E-4	0.0
6	0.0	0.0	0.0	5.277476521826324E-4	0.0
7	0.0	0.0	0.0	5.277476521826324E-4	0.0
8	0.0	0.0	0.0	5.277476521826324E-4	0.0
9	0.0	0.0	0.0	5.277476521826324E-4	0.0
10-11	0.0	0.0	0.0	6.596845652282904E-4	0.0
12-13	0.0	0.0	0.0	7.916214782739485E-4	0.0
14-15	0.0	0.0	0.0	7.916214782739485E-4	0.0
16-17	0.0	0.0	0.0	0.0011874322174109228	0.0
18-19	0.0	0.0	0.0	0.0013193691304565808	0.0
20-21	0.0	0.0	0.0	0.001583242956547897	0.0
22-23	0.0	0.0	0.0	0.004221981217461059	0.0
24-25	0.0	0.0	0.0	0.010159142304515673	0.0
26-27	0.0	0.0	0.0	0.014644997348068046	0.0
28-29	0.0	0.0	0.0	0.02163765373948793	0.0
30-31	0.0	0.0	0.0	0.04037269539197137	0.0
32-33	0.0	0.0	0.0	0.07573178808820774	0.0
34-35	0.0	0.0	0.0	0.11491705126276819	2.638738260913162E-4
36-37	0.0	0.0	0.0	0.17442059904635998	2.638738260913162E-4
38-39	0.0	0.0	0.0	0.2630822046130422	2.638738260913162E-4
40-41	0.0	0.0	0.0	0.34712601822312644	2.638738260913162E-4
42-43	0.0	0.0	0.0	0.4268159137027039	2.638738260913162E-4
44-45	0.0	0.0	0.0	0.5148178347041579	2.638738260913162E-4
46-47	0.0	0.0	0.0	0.615881510097132	2.638738260913162E-4
48-49	0.0	0.0	0.0	0.7275001385337587	2.638738260913162E-4
50-51	0.0	0.0	0.0	0.8257931387527739	2.638738260913162E-4
52-53	0.0	0.0	0.0	0.916037987276004	3.958107391369743E-4
54-55	0.0	0.0	0.0	1.006018961973143	5.277476521826324E-4
56-57	0.0	0.0	0.0	1.10972137562703	5.277476521826324E-4
58-59	0.0	0.0	0.0	1.2185693288896982	5.277476521826324E-4
60-61	0.0	0.0	0.0	1.3394235412395208	5.277476521826324E-4
62-63	0.0	0.0	0.0	1.4464243777195496	5.277476521826324E-4
64-65	0.0	0.0	0.0	1.550390665199528	5.277476521826324E-4
66-67	0.0	0.0	0.0	1.6634605996796572	5.277476521826324E-4
68-69	0.0	0.0	0.0	1.796584944942726	5.277476521826324E-4
70-71	0.0	0.0	0.0	1.9282579841622929	5.277476521826324E-4
72-73	0.0	0.0	0.0	2.0582158435122664	5.277476521826324E-4
74-75	0.0	0.0	0.0	2.1861946491665547	5.277476521826324E-4
76-77	0.0	0.0	0.0	2.31456926555998	5.277476521826324E-4
78-79	2.638738260913162E-4	0.0	0.0	2.465637030997258	5.277476521826324E-4
80-81	2.638738260913162E-4	0.0	0.0	2.6292388031738745	5.277476521826324E-4
82-83	2.638738260913162E-4	0.0	0.0	2.7989096733505905	5.277476521826324E-4
84-85	2.638738260913162E-4	0.0	0.0	2.962511445527207	6.596845652282904E-4
86-87	2.638738260913162E-4	0.0	0.0	3.136008486182247	9.235583913196066E-4
88-89	2.638738260913162E-4	0.0	0.0	3.319664669141803	0.0010554953043652648
90-91	3.958107391369743E-4	0.0	0.0	3.49843918631867	0.0010554953043652648
92-93	5.277476521826324E-4	0.0	0.0	3.687372845800052	0.0010554953043652648
94-95	5.277476521826324E-4	0.0	0.0	3.8875211428903156	0.0011874322174109228
96-97	5.277476521826324E-4	0.0	0.0	4.088197187632762	0.0013193691304565808
98-99	5.277476521826324E-4	0.0	0.0	4.295733951853581	0.0013193691304565808
100-101	5.277476521826324E-4	0.0	0.0	4.536254944335816	0.0013193691304565808
102-103	5.277476521826324E-4	0.0	0.0	4.774269135470183	0.0013193691304565808
104-105	5.277476521826324E-4	0.0	0.0	5.028379629996121	0.0013193691304565808
106-107	5.277476521826324E-4	0.0	0.0	5.285392736609063	0.0013193691304565808
108-109	5.277476521826324E-4	0.0	0.0	5.547815256656877	0.0013193691304565808
110-111	5.277476521826324E-4	0.0	0.0	5.84216650966174	0.0013193691304565808
112-113	5.277476521826324E-4	0.0	0.0	6.1433784821449775	0.0013193691304565808
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1445	0.0	47.001286	1
GTATCAA	1980	0.0	34.292355	2
GTCTAGG	120	3.6574304E-5	29.788095	1
TACCGAC	80	0.0053277346	29.78415	7
TATCAAC	2435	0.0	27.639942	3
ATCAACG	2450	0.0	26.498302	4
CAACGCA	2495	0.0	26.266054	6
TCTAGGA	205	7.664676E-8	26.14847	2
AACGCAG	2545	0.0	25.98067	7
TCAACGC	2620	0.0	24.785513	5
GTACAAA	265	1.3515091E-9	24.72974	1
ACGCAGA	2705	0.0	24.00349	8
CGCAGAG	2825	0.0	22.986921	9
CTAACGC	130	0.0020375676	22.907848	3
ACGCCTA	165	3.1821043E-4	21.664068	6
TTACACC	165	3.1878104E-4	21.65833	4
TCTAACG	140	0.0031332902	21.271574	2
CAGAGTA	2965	0.0	21.097807	10-11
GTACATG	3455	0.0	20.17486	1
GCAGAGT	2930	0.0	20.129839	9
>>END_MODULE