FastQCFastQC Report
Sun 1 Jan 2017
ERR1142734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378969
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25080.661795555837021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.5462188200090244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.3446192168752589No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8160.21532104209051403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.10238304452343068No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.061.972921
GTATCAA25600.043.4575922
CCGCAGA3050.040.967621
TATCAAC32000.034.766073
CTCAACG4150.034.409981
ATCAACG32500.034.231214
CAACGCA33250.033.633566
GTGGTAT7500.032.5269931
TCAACGC34850.032.089415
ACGTTAA750.00391206731.7252758
AACGCAG35550.031.6248827
ACGCAGA37000.030.0639848
CGCAGAG38350.029.005679
TGGTATC8750.027.8765962
GAGTACT18200.026.80088612-13
CAGAGTA40150.025.92741410-11
TATTCTC2201.5367914E-724.3347285
GCAGAGT40500.023.7939579
GTACTTT21200.023.00830814-15
GTCCTAG1653.2095617E-421.636581
Produced by FastQC (version 0.11.5)