FastQCFastQC Report
Sun 1 Jan 2017
ERR1142733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176238
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5260.29846003699542667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3830.2173197607780388No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3260.1849771331948842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3180.1804378170428625No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA2480.14071880071267262No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2330.13220758292763196No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2130.1208592925475777No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1950.11064583120552887No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA1890.10724134409151262No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1880.1066739295725099No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1870.1061065150535072No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1800.1021346134204882No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGT902.3660273E-433.100061
TATTCTC1102.011415E-532.498245
CCCCAGG750.003875940331.7760561
AGTAGTA750.003875940331.7760562
CTAACGC953.249349E-431.3579523
CGCCTAC1004.3814775E-429.7985347
TAACGCC1004.3887924E-429.7900564
TCTAACG1004.3887924E-429.7900562
GGTATCA13050.027.3931521
ACGCCTA1107.6644955E-427.081876
AGTAGTG900.00944527626.480055
TAGTAGT900.00944527626.480054
GCATACA900.00944527626.480054
GTCATAC450.00952369926.44242552-53
ACTGCAA450.00955011926.427404106-107
CCTATTT1701.37559855E-524.5329883
CTATTGA2052.21343E-623.2573939
AGGGCTA2052.2189197E-623.2507765
CATGGGC2602.9654984E-822.9154264
GTATCAA15900.022.4830591
Produced by FastQC (version 0.11.5)