FastQCFastQC Report
Sun 1 Jan 2017
ERR1142733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176238
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.7603354554636345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.7194816100954391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.431802448961064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4390.24909497384219068No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.236611854424131No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3520.19972991068895474No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC2290.12993792485162112No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC2230.12653343773760484No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA2030.11518514735755059No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1900.10780875861051532No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1900.10780875861051532No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1900.10780875861051532No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.1066739295725099No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGACT300.00423480659.4851044
ATAAGAC350.00779307750.9872323
GGTATCA15300.034.6319271
GCACCGG700.002786031733.991496
GCGTAAT350.002789798433.9914910-11
CATGGGT1102.0335216E-532.446424
CTAACGC750.003906368331.7253883
CCGCAGA2450.031.5904161
GTATCAA20400.030.643421
AAGACTC800.00535659229.7425525
TCTAACG800.00535659229.7425522
CTCAACG2451.6370905E-1129.1603851
AGCACCG850.00720286927.992995
CACCGGC850.00720286927.992997
TATCAAC23500.026.8315772
ATCAACG23500.026.8315773
GTGTTGT900.00947965426.460351
GTTTAAG450.00953179926.43782252-53
TCGGGGC450.00953179926.43782228-29
CGCCTAC900.00951911226.4378227
Produced by FastQC (version 0.11.5)