##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142733_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	176238
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.508863014786822	14.0	14.0	33.0	14.0	33.0
2	27.278674292717803	27.0	27.0	33.0	14.0	33.0
3	31.01280654569389	33.0	27.0	33.0	27.0	33.0
4	30.50639476162916	33.0	33.0	33.0	27.0	33.0
5	32.216593470193715	33.0	33.0	33.0	33.0	33.0
6	34.83368513033511	37.0	37.0	37.0	27.0	37.0
7	35.04287951520104	37.0	37.0	37.0	33.0	37.0
8	35.39998184273539	37.0	37.0	37.0	33.0	37.0
9	35.900889705965795	37.0	37.0	37.0	33.0	37.0
10-11	36.00081707690737	37.0	37.0	37.0	33.0	37.0
12-13	36.07040763059045	37.0	37.0	37.0	35.0	37.0
14-15	36.07396815669719	37.0	37.0	37.0	37.0	37.0
16-17	36.088130823091504	37.0	37.0	37.0	37.0	37.0
18-19	36.10345952632236	37.0	37.0	37.0	37.0	37.0
20-21	36.10881591938174	37.0	37.0	37.0	37.0	37.0
22-23	36.11131538033795	37.0	37.0	37.0	37.0	37.0
24-25	36.09263609437238	37.0	37.0	37.0	37.0	37.0
26-27	35.97833611366448	37.0	37.0	37.0	35.0	37.0
28-29	35.91975340165004	37.0	37.0	37.0	33.0	37.0
30-31	35.92497928937006	37.0	37.0	37.0	33.0	37.0
32-33	35.85608949261794	37.0	37.0	37.0	33.0	37.0
34-35	35.73962482552004	37.0	37.0	37.0	33.0	37.0
36-37	35.666033999477975	37.0	37.0	37.0	33.0	37.0
38-39	35.56415472259104	37.0	37.0	37.0	33.0	37.0
40-41	35.46588420204496	37.0	37.0	37.0	33.0	37.0
42-43	35.33798896946175	37.0	37.0	37.0	33.0	37.0
44-45	35.19022855456825	37.0	37.0	37.0	33.0	37.0
46-47	34.95037392616803	37.0	37.0	37.0	30.0	37.0
48-49	34.752309377092345	37.0	37.0	37.0	27.0	37.0
50-51	34.635529794936396	37.0	37.0	37.0	27.0	37.0
52-53	34.54936790022583	37.0	37.0	37.0	27.0	37.0
54-55	34.4779786425175	37.0	37.0	37.0	27.0	37.0
56-57	34.42932568458562	37.0	37.0	37.0	27.0	37.0
58-59	34.34992169679638	37.0	37.0	37.0	27.0	37.0
60-61	34.311028268591336	37.0	37.0	37.0	27.0	37.0
62-63	34.30249435422553	37.0	37.0	37.0	27.0	37.0
64-65	34.27774940705183	37.0	37.0	37.0	27.0	37.0
66-67	34.26480100772818	37.0	37.0	37.0	27.0	37.0
68-69	34.24503796003132	37.0	37.0	37.0	27.0	37.0
70-71	34.20588919529273	37.0	37.0	37.0	27.0	37.0
72-73	34.17024421520898	37.0	37.0	37.0	27.0	37.0
74-75	34.082468026191854	37.0	37.0	37.0	27.0	37.0
76-77	33.99940137768245	37.0	37.0	37.0	27.0	37.0
78-79	33.99732180347031	37.0	37.0	37.0	27.0	37.0
80-81	33.941700995245064	37.0	37.0	37.0	22.0	37.0
82-83	33.91883702720185	37.0	37.0	37.0	22.0	37.0
84-85	33.8217467288553	37.0	37.0	37.0	22.0	37.0
86-87	33.685249492164004	37.0	37.0	37.0	22.0	37.0
88-89	33.62476026736572	37.0	37.0	37.0	22.0	37.0
90-91	33.572141081946	37.0	33.0	37.0	14.0	37.0
92-93	33.44434230983103	37.0	33.0	37.0	14.0	37.0
94-95	33.4668289472191	37.0	33.0	37.0	14.0	37.0
96-97	33.476999852472225	37.0	33.0	37.0	14.0	37.0
98-99	33.40668868235001	37.0	33.0	37.0	14.0	37.0
100-101	33.307995437987266	37.0	33.0	37.0	14.0	37.0
102-103	33.1918513600926	37.0	33.0	37.0	14.0	37.0
104-105	33.090057195383515	37.0	33.0	37.0	14.0	37.0
106-107	32.962800304134184	37.0	33.0	37.0	14.0	37.0
108-109	32.84580510446101	37.0	33.0	37.0	14.0	37.0
110-111	32.81491222097391	37.0	33.0	37.0	14.0	37.0
112-113	32.74863253100921	37.0	33.0	37.0	14.0	37.0
114-115	32.65033931388236	37.0	33.0	37.0	14.0	37.0
116-117	32.53245894755955	37.0	33.0	37.0	14.0	37.0
118-119	32.40758519729003	37.0	33.0	37.0	14.0	37.0
120-121	32.31561297790488	37.0	33.0	37.0	14.0	37.0
122-123	32.186585753356255	37.0	33.0	37.0	14.0	37.0
124-125	30.917670990365302	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	11.0
16	4.0
17	7.0
18	34.0
19	179.0
20	788.0
21	1747.0
22	2724.0
23	3512.0
24	2593.0
25	1716.0
26	1550.0
27	1621.0
28	2092.0
29	2772.0
30	3609.0
31	4889.0
32	7020.0
33	10672.0
34	17879.0
35	37079.0
36	73740.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.58258263372103	14.329422316071499	13.459235166232425	28.628759883975047
2	17.363451696001995	20.879719470261804	39.358708110623134	22.398120723113063
3	19.814682418093714	27.950839206073606	29.696206266525948	22.538272109306735
4	14.00330237123873	19.49023190362977	35.13734346363136	31.369122261500138
5	15.149245261604813	35.715015321756894	33.163659062535466	15.972080354102827
6	27.275358042624664	35.66581175242289	19.642411312104223	17.416418892848228
7	23.818926678695853	33.48767008250207	23.99085327795368	18.702549960848398
8	25.933680591018966	31.561297790487863	21.561184307584064	20.94383731090911
9	26.78707202759904	15.885904288518935	21.82446464440132	35.5025590394807
10-11	25.192920936460922	25.696501322075832	26.51613159477525	22.594446146688004
12-13	26.854026940841365	25.17447996459333	26.787923149377548	21.18356994518776
14-15	24.67515518787095	24.62805978279372	26.99985247222506	23.69693255711027
16-17	22.060225377046947	26.732883941034284	28.055810892089106	23.151079789829662
18-19	22.021924897014262	24.97049444501186	31.497463657100056	21.51011700087382
20-21	23.779058880938734	23.644297168018067	31.420189857975338	21.156454093067858
22-23	24.182639385376593	23.391947253146313	30.929198016318843	21.49621534515825
24-25	22.826802392219612	24.543231312202817	31.15673123843893	21.47323505713864
26-27	22.098525857079633	24.96652254337884	31.320430327171213	21.614521272370318
28-29	21.72147890920233	24.57755989060248	31.92926610606112	21.77169509413407
30-31	22.672749350310376	24.028302636207854	31.724429464701142	21.574518548780627
32-33	22.35420951840556	24.48882899496418	31.106319597134547	22.050641889495708
34-35	22.08150342150955	24.45386352545989	31.770106333480864	21.6945267195497
36-37	22.418391143743936	24.6364270839835	31.224430738153735	21.72075103411883
38-39	22.29992737011734	24.532728840872466	31.35653328491341	21.81081050409678
40-41	21.655360643691466	24.236027769975628	31.591042565687	22.517569020645904
42-43	22.324830554397074	24.480172949417405	30.747319652852845	22.44767684333268
44-45	22.54564849805377	24.697851768631054	30.508176443218833	22.248323290096348
46-47	22.41173867156913	24.654728265186847	30.021618493174003	22.911914570070017
48-49	22.881841600562876	24.68792201454851	29.890545739284374	22.539690645604242
50-51	22.30563215651562	25.103836856977495	29.457608461285307	23.132922525221574
52-53	22.02532938412828	25.192353521941918	28.75968860291197	24.022628491017826
54-55	21.908441993213724	25.32910042102157	28.7446521181584	24.017805467606305
56-57	22.67501900838639	24.881410365528435	28.35710800167955	24.086462624405634
58-59	22.385197206035055	24.978579980367346	29.130091864931885	23.506130948665717
60-61	22.69686446736799	24.9795730773159	29.430656271632678	22.89290618368343
62-63	22.233286805342775	24.887935632496962	29.15120462102384	23.72757294113642
64-65	21.582746059306164	25.005390437930526	29.503569037324528	23.908294465438782
66-67	22.565791713478365	24.483652787707534	29.183547248606995	23.767008250207105
68-69	21.892554386681645	24.38662490495807	29.579886290130393	24.140934418229893
70-71	22.149214266057257	24.54429133579026	29.572194182249422	23.734300215903062
72-73	22.825383855922105	24.53358526537977	29.921469830570025	22.7195610481281
74-75	22.550208272403836	24.526737519982508	30.02217597101507	22.900878236598587
76-77	22.110470002953136	24.379557483928124	30.13504918107267	23.37492333204607
78-79	21.638475462019894	24.906801636432757	30.15484830086758	23.299874600679765
80-81	21.565723623736087	25.13220758292763	30.068997605510727	23.233071187825555
82-83	22.04241435562806	24.636924604581885	30.436768565146465	22.88389247464359
84-85	22.481456660267842	24.86511353181049	29.533037245703508	23.120392562218157
86-87	21.627090704977903	25.30388938625286	29.807733299228374	23.261286609540864
88-89	22.282846663444314	24.35586136305479	29.78505018338972	23.576241790111172
90-91	22.517276428534924	24.152077998035285	29.847479089417227	23.48316648401256
92-93	22.40762101746152	24.613465767357816	29.58258280441169	23.39633041076898
94-95	21.05636460097653	24.855237138731194	29.93846337166251	24.14993488862977
96-97	21.486285591075706	24.637705829616767	29.39207208434049	24.483936494967033
98-99	21.494796808860745	25.085112177850405	28.92906183683428	24.491029176454568
100-101	21.902484140764194	25.179019280745358	28.66549779275752	24.25299878573293
102-103	22.166962195900417	25.19384353500248	28.679764522306545	23.959429746790555
104-105	22.15154498486366	25.42082431347404	28.450362165674125	23.977268535988173
106-107	22.010094733885452	25.15540726491725	28.451658481363896	24.3828395198334
108-109	22.03994314103237	25.668248919707988	27.922462980669653	24.369344958589988
110-111	21.56799881973092	26.276024082028698	27.917052051001246	24.238925047239135
112-113	21.424092741649478	26.405608531959384	27.468415124424407	24.70188360196673
114-115	21.76647827863271	26.1791275137319	27.330167052521677	24.724227155113716
116-117	22.199840554258365	25.644095542612018	27.450201576867318	24.705862326262302
118-119	21.521770585381493	25.667076156619977	27.840304592434556	24.970848665563974
120-121	21.742473246405428	25.66500981627118	28.34263893144498	24.249878005878415
122-123	21.47011427728413	26.259943939445524	28.174684233820173	24.095257549450174
124-125	21.363217249450315	25.842967586353645	28.259876586991982	24.533938577204058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	64.5
2	104.0
3	140.5
4	111.0
5	108.5
6	141.0
7	325.5
8	564.5
9	676.5
10	615.5
11	483.5
12	464.0
13	466.5
14	459.0
15	453.0
16	479.5
17	489.5
18	465.0
19	450.5
20	436.0
21	444.5
22	444.0
23	463.5
24	476.0
25	489.0
26	505.0
27	507.0
28	602.0
29	837.0
30	1204.5
31	1544.5
32	1992.0
33	2451.0
34	2858.5
35	3208.5
36	3546.5
37	4296.5
38	4813.5
39	4541.5
40	4122.0
41	3852.0
42	3876.0
43	4314.5
44	4908.0
45	5628.5
46	6453.0
47	7258.5
48	7825.5
49	8075.5
50	8593.5
51	8461.0
52	7625.0
53	7312.5
54	7060.5
55	6546.0
56	6190.0
57	5223.5
58	4090.0
59	3574.0
60	2927.0
61	2321.0
62	1801.0
63	1377.5
64	1016.5
65	697.5
66	465.0
67	335.5
68	275.5
69	238.0
70	212.0
71	165.5
72	135.5
73	134.0
74	105.0
75	82.5
76	72.5
77	45.0
78	31.5
79	25.5
80	11.5
81	8.0
82	6.5
83	1.5
84	0.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039151601811187146
2	0.0
3	0.0
4	5.674145190027123E-4
5	0.01021346134204882
6	0.0011348290380054245
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.674145190027123E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.837072595013561E-4
34-35	0.0
36-37	5.674145190027123E-4
38-39	0.0011348290380054245
40-41	0.002553365335512205
42-43	8.511217785040683E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.674145190027123E-4
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	8.511217785040683E-4
72-73	0.0
74-75	0.08312622703389734
76-77	0.08738183592641768
78-79	5.674145190027123E-4
80-81	0.0
82-83	2.837072595013561E-4
84-85	0.09305598111644481
86-87	0.17618220815034216
88-89	0.2173197607780388
90-91	0.07433130198935531
92-93	0.14242104426968077
94-95	8.511217785040683E-4
96-97	0.0
98-99	0.0
100-101	0.0
102-103	2.837072595013561E-4
104-105	0.0036881943735176297
106-107	0.004255608892520342
108-109	0.007092681487533903
110-111	0.003971901633018985
112-113	0.0036881943735176297
114-115	0.0034044871140162733
116-117	0.0014185362975067805
118-119	0.0014185362975067805
120-121	0.0
122-123	0.0
124-125	2.837072595013561E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	176238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.453012403681385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18765417933567	39.16068044349119
2	11.18467098846882	11.062313462476878
3	3.14152945900981	4.660742859088279
4	1.445700189317882	2.85976917577367
5	0.9110205954907923	2.2526356404407677
6	0.5691010269060869	1.6886256085520719
7	0.4612472032585624	1.5967044564736323
8	0.34995123630313807	1.384491426366618
9	0.2581607480924789	1.1490144009804923
>10	2.2591933910848487	21.89141955764364
>50	0.16178073547128677	5.412567096766872
>100	0.06654810395272791	4.969416357425754
>500	0.0011473811026332396	0.431802448961064
>1k	0.0022947622052664793	1.4798170655590737
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1340	0.7603354554636345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.7194816100954391	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	761	0.431802448961064	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	439	0.24909497384219068	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.236611854424131	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	352	0.19972991068895474	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	229	0.12993792485162112	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	223	0.12653343773760484	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	203	0.11518514735755059	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	190	0.10780875861051532	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	190	0.10780875861051532	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	190	0.10780875861051532	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	188	0.1066739295725099	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	5.674145190027123E-4	2.837072595013561E-4
16-17	0.0	0.0	0.0	5.674145190027123E-4	0.0011348290380054245
18-19	0.0	0.0	0.0	5.674145190027123E-4	0.0011348290380054245
20-21	0.0	0.0	0.0	5.674145190027123E-4	0.0011348290380054245
22-23	0.0	0.0	0.0	0.0034044871140162733	0.0011348290380054245
24-25	0.0	0.0	0.0	0.011915704899056957	0.0011348290380054245
26-27	0.0	0.0	0.0	0.017022435570081366	0.0011348290380054245
28-29	0.0	0.0	0.0	0.03007296950714375	0.0011348290380054245
30-31	0.0	0.0	0.0	0.059578524495284786	0.0011348290380054245
32-33	0.0	0.0	0.0	0.10185090616098685	0.0011348290380054245
34-35	0.0	0.0	0.0	0.15008114027621738	0.0011348290380054245
36-37	0.0	0.0	0.0	0.2385978052406405	0.0011348290380054245
38-39	0.0	0.0	0.0	0.35775485423121006	0.0011348290380054245
40-41	0.0	0.0	0.0	0.477195610481281	0.0011348290380054245
42-43	0.0	0.0	0.0	0.5756420295282516	0.0011348290380054245
44-45	0.0	0.0	0.0	0.6797625937652493	0.0011348290380054245
46-47	0.0	0.0	0.0	0.8057286169838513	0.0011348290380054245
48-49	0.0	0.0	0.0	0.9314109329429521	0.0011348290380054245
50-51	0.0	0.0	0.0	1.0497168601550175	0.0011348290380054245
52-53	0.0	0.0	0.0	1.174264347076113	0.0011348290380054245
54-55	0.0	0.0	0.0	1.282640520205631	0.0011348290380054245
56-57	0.0	0.0	0.0	1.4009464474176965	0.0011348290380054245
58-59	0.0	0.0	0.0	1.5334377376048298	0.0011348290380054245
60-61	0.0	0.0	0.0	1.669049807646478	0.0011348290380054245
62-63	0.0	0.0	0.0	1.7882068566370477	0.0011348290380054245
64-65	0.0	0.0	0.0	1.902540882216094	0.0011348290380054245
66-67	0.0	0.0	0.0	2.0412737321122574	0.0011348290380054245
68-69	0.0	0.0	0.0	2.1941919449834884	0.0011348290380054245
70-71	0.0	0.0	0.0	2.356472497418264	0.0011348290380054245
72-73	0.0	0.0	0.0	2.515915977258026	0.0011348290380054245
74-75	0.0	0.0	0.0	2.666564532053246	0.0011348290380054245
76-77	0.0	0.0	0.0	2.841611911165583	0.0011348290380054245
78-79	0.0	0.0	0.0	3.0177941193159254	0.0011348290380054245
80-81	0.0	0.0	0.0	3.224049296973411	0.0011348290380054245
82-83	0.0	0.0	0.0	3.4328578399664087	0.0011348290380054245
84-85	0.0	0.0	0.0	3.6172675586422907	0.0011348290380054245
86-87	0.0	0.0	0.0	3.8099047878437116	0.0011348290380054245
88-89	0.0	0.0	0.0	4.009350991273164	0.0011348290380054245
90-91	0.0	0.0	0.0	4.2385864569502605	0.0011348290380054245
92-93	0.0	0.0	0.0	4.468389337146359	0.0011348290380054245
94-95	0.0	0.0	0.0	4.680886074512875	0.0014185362975067807
96-97	0.0	0.0	0.0	4.9089867111519645	0.0017022435570081366
98-99	0.0	0.0	0.0	5.167727731817202	0.0017022435570081366
100-101	0.0	0.0	0.0	5.447463089685539	0.0017022435570081366
102-103	0.0	0.0	0.0	5.734291129041409	0.0017022435570081366
104-105	0.0	0.0	0.0	6.028211849884815	0.0017022435570081366
106-107	0.0	0.0	0.0	6.32837413043725	0.0017022435570081366
108-109	0.0	0.0	0.0	6.608393195565088	0.0017022435570081366
110-111	0.0	0.0	0.0	6.93096834961813	0.0017022435570081366
112-113	0.0	0.0	0.0	7.263189550494218	0.0017022435570081366
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGACT	30	0.004234806	59.485104	4
ATAAGAC	35	0.007793077	50.987232	3
GGTATCA	1530	0.0	34.631927	1
GCACCGG	70	0.0027860317	33.99149	6
GCGTAAT	35	0.0027897984	33.99149	10-11
CATGGGT	110	2.0335216E-5	32.44642	4
CTAACGC	75	0.0039063683	31.725388	3
CCGCAGA	245	0.0	31.590416	1
GTATCAA	2040	0.0	30.64342	1
AAGACTC	80	0.005356592	29.742552	5
TCTAACG	80	0.005356592	29.742552	2
CTCAACG	245	1.6370905E-11	29.160385	1
AGCACCG	85	0.007202869	27.99299	5
CACCGGC	85	0.007202869	27.99299	7
TATCAAC	2350	0.0	26.831577	2
ATCAACG	2350	0.0	26.831577	3
GTGTTGT	90	0.009479654	26.46035	1
GTTTAAG	45	0.009531799	26.437822	52-53
TCGGGGC	45	0.009531799	26.437822	28-29
CGCCTAC	90	0.009519112	26.437822	7
>>END_MODULE