FastQCFastQC Report
Sun 1 Jan 2017
ERR1142728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470741
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12460.2646890753089278No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8810.18715174586449873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27550.030.2715931
GGTATCA20350.030.1509971
TCTAACG800.00532821729.7850862
GCCGTGT800.005330995729.781918
ATGGGTA4850.029.4780225
GGGTACC2850.029.2625397
TGGGTAC3600.026.475636
CTAACGC900.00946876226.475633
CATGGGT13650.025.748284
CATGGGG19700.025.0980934
TATCAAC33150.023.8999482
GTACATG49450.023.8521631
GTAAGTA1501.6711118E-423.8280682
ATGGGGG10850.023.6084565
TACATGG49500.023.5873832
ACATGGG47850.023.404793
CAACGCA32000.023.26965
ATCAACG32250.023.0892123
AACGCAG32350.023.017846
GGTACCT3900.022.9091638
Produced by FastQC (version 0.11.5)