##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142728_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470741
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.446211398624722	33.0	33.0	33.0	27.0	33.0
2	31.545616379282876	33.0	33.0	33.0	27.0	33.0
3	31.585281502992093	33.0	33.0	33.0	27.0	33.0
4	31.515170762691163	33.0	33.0	33.0	27.0	33.0
5	31.594719814080353	33.0	33.0	33.0	27.0	33.0
6	34.978327785342685	37.0	37.0	37.0	33.0	37.0
7	34.974070667309626	37.0	37.0	37.0	33.0	37.0
8	34.928357631903744	37.0	37.0	37.0	33.0	37.0
9	34.83586090865253	37.0	37.0	37.0	27.0	37.0
10-11	34.90767109727005	37.0	37.0	37.0	33.0	37.0
12-13	34.86389968156587	37.0	37.0	37.0	30.0	37.0
14-15	34.861053105635584	37.0	37.0	37.0	27.0	37.0
16-17	34.90772739149554	37.0	37.0	37.0	33.0	37.0
18-19	34.875384765720426	37.0	37.0	37.0	27.0	37.0
20-21	34.89976016535632	37.0	37.0	37.0	27.0	37.0
22-23	34.98678360287292	37.0	37.0	37.0	27.0	37.0
24-25	35.037995415738166	37.0	37.0	37.0	33.0	37.0
26-27	35.10322980152568	37.0	37.0	37.0	33.0	37.0
28-29	35.14406542026295	37.0	37.0	37.0	33.0	37.0
30-31	35.1836615038843	37.0	37.0	37.0	33.0	37.0
32-33	35.17727901330031	37.0	37.0	37.0	33.0	37.0
34-35	35.17183546791123	37.0	37.0	37.0	33.0	37.0
36-37	35.16735104866582	37.0	37.0	37.0	33.0	37.0
38-39	35.13857089142437	37.0	37.0	37.0	33.0	37.0
40-41	35.11930764475582	37.0	37.0	37.0	33.0	37.0
42-43	35.113205563144064	37.0	37.0	37.0	33.0	37.0
44-45	35.117833373341185	37.0	37.0	37.0	33.0	37.0
46-47	35.12403423538633	37.0	37.0	37.0	33.0	37.0
48-49	35.0870223753614	37.0	37.0	37.0	33.0	37.0
50-51	35.083339883290385	37.0	37.0	37.0	33.0	37.0
52-53	35.07066518531422	37.0	37.0	37.0	33.0	37.0
54-55	35.044530856670654	37.0	37.0	37.0	33.0	37.0
56-57	35.024322291876	37.0	37.0	37.0	33.0	37.0
58-59	34.9914815153131	37.0	37.0	37.0	27.0	37.0
60-61	34.981095761788325	37.0	37.0	37.0	27.0	37.0
62-63	34.92551530459424	37.0	37.0	37.0	27.0	37.0
64-65	34.906308352151186	37.0	37.0	37.0	27.0	37.0
66-67	34.850120342183914	37.0	37.0	37.0	27.0	37.0
68-69	34.8209694927784	37.0	37.0	37.0	27.0	37.0
70-71	34.7738225478554	37.0	37.0	37.0	27.0	37.0
72-73	34.73956804272413	37.0	37.0	37.0	27.0	37.0
74-75	34.690533648014515	37.0	37.0	37.0	27.0	37.0
76-77	34.63634036550884	37.0	37.0	37.0	27.0	37.0
78-79	34.571406569642335	37.0	37.0	37.0	27.0	37.0
80-81	34.527421660743386	37.0	37.0	37.0	27.0	37.0
82-83	34.489350831986165	37.0	37.0	37.0	27.0	37.0
84-85	34.46264400169095	37.0	37.0	37.0	27.0	37.0
86-87	34.39166335628296	37.0	37.0	37.0	27.0	37.0
88-89	34.33605846951933	37.0	37.0	37.0	27.0	37.0
90-91	34.263293403378924	37.0	37.0	37.0	27.0	37.0
92-93	34.22541057609173	37.0	37.0	37.0	27.0	37.0
94-95	34.177728304949	37.0	37.0	37.0	27.0	37.0
96-97	34.091860492287694	37.0	37.0	37.0	27.0	37.0
98-99	34.01740978584827	37.0	37.0	37.0	22.0	37.0
100-101	33.94891777006889	37.0	37.0	37.0	22.0	37.0
102-103	33.886369574776786	37.0	37.0	37.0	22.0	37.0
104-105	33.738146879069376	37.0	33.0	37.0	22.0	37.0
106-107	33.66326281330923	37.0	33.0	37.0	22.0	37.0
108-109	33.56461727361755	37.0	33.0	37.0	22.0	37.0
110-111	33.464658910101306	37.0	33.0	37.0	22.0	37.0
112-113	33.37915116805207	37.0	33.0	37.0	22.0	37.0
114-115	33.25282161528314	37.0	33.0	37.0	22.0	37.0
116-117	33.15074743861274	37.0	33.0	37.0	18.0	37.0
118-119	33.046591437754515	37.0	33.0	37.0	14.0	37.0
120-121	32.9186123579633	37.0	33.0	37.0	14.0	37.0
122-123	32.8101578150193	37.0	33.0	37.0	14.0	37.0
124-125	31.204482932228125	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	845.0
1	44.0
2	21.0
3	21.0
4	10.0
5	23.0
6	16.0
7	12.0
8	13.0
9	14.0
10	9.0
11	16.0
12	13.0
13	14.0
14	47.0
15	173.0
16	266.0
17	473.0
18	944.0
19	1678.0
20	2487.0
21	3272.0
22	3490.0
23	3552.0
24	3889.0
25	4469.0
26	5213.0
27	6463.0
28	7549.0
29	9020.0
30	10978.0
31	13458.0
32	17017.0
33	23497.0
34	36138.0
35	70885.0
36	244712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.736189743600676	20.155889428455147	12.467318833940256	23.640601994003923
2	17.17512535732696	23.221672652292643	36.231569171257064	23.37163281912333
3	19.41709728677382	28.24583899565049	28.476307729009665	23.860755988566023
4	13.955796985673963	19.465942376311446	34.81557224263727	31.762688395377324
5	14.616462988529255	38.701828842187794	31.25926778135706	15.422440387925892
6	28.846514562444582	37.037250187572475	18.62253597981038	15.493699270172566
7	26.023241584998498	34.05674596491377	21.698541591607277	18.221470858480448
8	25.627519403805938	34.14110579843917	20.408476494801846	19.822898302953046
9	26.639252527707253	18.271544933820223	19.982263947422837	35.10693859104969
10-11	24.923548867436025	27.59328956779129	25.77274534444846	21.710416220324223
12-13	25.6060368637559	26.688114204943382	25.414799867391963	22.291049063908762
14-15	24.35028218478707	26.851947581311318	23.500370384186994	25.29739984971462
16-17	23.713794513894214	28.76903477601384	24.233602610970507	23.28356809912144
18-19	23.723024226273782	27.87651054747161	25.84040012866147	22.560065097593135
20-21	25.89136819507125	26.419554832375287	25.011128125725456	22.677948846828006
22-23	25.254466676818005	26.311199515291428	25.10943744203356	23.324896365857008
24-25	24.132295288979368	27.23588748746276	25.090449125960124	23.541368097597747
26-27	23.930314301199726	27.230066944867115	25.192332060170518	23.647286693762638
28-29	23.55674844666272	27.130382382876878	25.663176005144432	23.64969316531597
30-31	24.115907280989028	26.93523041347092	25.604726233620223	23.344136071919834
32-33	23.721992001669513	27.0195997427587	25.205601386718	24.052806868853786
34-35	23.99672513026018	27.150331000325785	25.488302667805137	23.3646412016089
36-37	23.949226451813463	27.252189910018863	25.044607306655536	23.753976331512135
38-39	23.89713319577443	27.046827521150213	25.217357974690014	23.838681308385343
40-41	23.565198137530633	27.319730295359367	25.484618872937777	23.630452694172224
42-43	23.893945684690152	27.212694154344053	25.349557663440965	23.543802497524833
44-45	23.787382346867304	27.259653358370215	25.184636379700535	23.768327915061942
46-47	23.663620763803433	27.149873749739196	25.205555863079145	23.98094962337823
48-49	23.73156160043605	27.371868027048663	25.265397979866798	23.631172392648487
50-51	23.422192886267577	27.571302338950932	25.212017331871266	23.794487442910224
52-53	23.486997031908054	27.173158569354133	25.291699136826058	24.048145261911756
54-55	23.490591648258057	27.094988422537458	25.462433130173263	23.951986799031218
56-57	23.842635962596447	26.809499071724208	25.02224881197731	24.325616153702033
58-59	23.64326601961179	26.980634130814096	25.458064392499786	23.91803545707433
60-61	23.855621526036693	27.077032158746484	25.439764763246174	23.62758155197065
62-63	23.45538378662658	27.127969330711554	25.26564684114207	24.151000041519797
64-65	23.410754171803568	26.80632653452318	25.56058776810372	24.222331525569533
66-67	23.91229347951087	26.633316722249905	25.43369835124349	24.020691446995738
68-69	23.8675989722917	26.58689469610095	25.263289028585667	24.28221730302169
70-71	23.528359524815983	26.65962501051457	25.556739812579792	24.255275652089658
72-73	23.935462780462924	26.81604102577208	25.336459674353673	23.91203651941132
74-75	23.917201046966543	26.607027624497654	25.587108425780592	23.888662902755208
76-77	23.748771530819006	26.397260580274008	25.713363934593797	24.140603954313193
78-79	23.85350993292864	26.584705941229775	25.567934374630653	23.993849751210934
80-81	23.651771710763786	26.440064013492666	25.867967379950745	24.040196895792807
82-83	23.796083458344825	26.61387260220155	25.745091546739037	23.84495239271459
84-85	23.747079081700548	26.68636124107756	25.401955618010426	24.164604059211474
86-87	23.42988586987761	26.77384949130027	25.67619063839209	24.120074000430037
88-89	23.62283670732486	26.187176703493197	25.714924324669518	24.47506226451242
90-91	24.139410267573442	26.231181668286418	25.471156845066705	24.158251219073428
92-93	23.698316363566697	26.719665699499956	25.514680994641907	24.06733694229144
94-95	23.086960316227074	26.80128738646499	25.820315077619956	24.291437219687985
96-97	23.365492464632403	26.628441782607677	25.595135479119975	24.41093027363994
98-99	23.138773681393005	26.881287854841744	25.575638377239894	24.404300086525353
100-101	23.374523875153386	27.126584555196427	25.330640037376934	24.168251532273256
102-103	23.5075440954695	27.055189977958914	25.62321135706448	23.814054569507103
104-105	23.419382717889007	27.1708009681354	25.472413037045865	23.937403276929725
106-107	23.34048886812353	27.189045539639782	25.708201051725254	23.76226454051143
108-109	23.335990710179527	27.21508680468706	25.456960606605694	23.99196187852772
110-111	23.113951533082876	27.55111163142155	25.360202637264393	23.974734198231182
112-113	23.25597730189711	27.604779713038425	25.247914622439705	23.891328362624755
114-115	23.270446275679237	27.641570377843834	25.256566211270286	23.831417135206642
116-117	23.2150725081895	27.729247417567564	25.250580555076635	23.8050995191663
118-119	23.008093309849787	27.40519227597069	25.52462845801429	24.06208595616523
120-121	23.098724358933424	27.360084971786048	25.711202355029663	23.829988314250865
122-123	23.21725980484493	27.61958276531054	25.463277792144627	23.6998796376999
124-125	23.216777708350854	27.388212301623206	25.396700364154274	23.998309625871666
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1555.0
1	988.0
2	331.5
3	212.5
4	171.0
5	146.0
6	139.5
7	129.0
8	128.5
9	136.0
10	115.0
11	104.5
12	110.5
13	117.0
14	119.0
15	136.5
16	156.0
17	167.5
18	202.5
19	239.0
20	271.0
21	325.0
22	423.5
23	583.0
24	763.5
25	946.0
26	1217.0
27	1586.5
28	2028.5
29	2713.0
30	3786.5
31	4827.5
32	5986.5
33	7293.0
34	8484.5
35	9500.0
36	10264.0
37	11222.5
38	11591.5
39	11491.0
40	11552.5
41	11492.5
42	11682.0
43	12429.0
44	13593.5
45	14675.5
46	15910.5
47	17035.5
48	18310.0
49	19404.0
50	19724.5
51	19145.0
52	18454.5
53	18649.0
54	18261.0
55	17191.0
56	16544.5
57	15270.5
58	13808.5
59	12629.0
60	10899.5
61	8954.5
62	7140.5
63	5514.0
64	4246.0
65	3493.5
66	2776.0
67	2114.5
68	1716.0
69	1442.0
70	1193.0
71	1039.5
72	919.5
73	887.5
74	705.0
75	559.0
76	472.0
77	349.5
78	234.5
79	120.0
80	83.0
81	64.0
82	42.5
83	27.0
84	18.5
85	11.0
86	10.5
87	6.5
88	2.5
89	1.0
90	0.5
91	1.5
92	1.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3046261107487982
2	0.27254902377315765
3	0.26830040298168206
4	0.2795592480790923
5	0.2916678173347977
6	0.33755292188273384
7	0.35433497400906233
8	0.3466874565844063
9	0.3483869049009965
10-11	0.3506174308165212
12-13	0.3589022413598985
14-15	0.34700610314376695
16-17	0.28402029991014166
18-19	0.27446090312932164
20-21	0.2570415578842718
22-23	0.2507748422168454
24-25	0.24217138511410732
26-27	0.24971268701897645
28-29	0.23516116080817265
30-31	0.23516116080817265
32-33	0.24280867823282867
34-35	0.2345238676894513
36-37	0.23091254001669706
38-39	0.2387724884809269
40-41	0.22135314323587707
42-43	0.22857579858138552
44-45	0.21965369491928682
46-47	0.22071585011715572
48-49	0.22708878130436905
50-51	0.23070010897712329
52-53	0.22921309170010687
54-55	0.22910687618031997
56-57	0.2236898846711886
58-59	0.22825715202202485
60-61	0.2308063244969102
62-63	0.2305938934573364
64-65	0.23367414353115618
66-67	0.24960647149918957
68-69	0.2448267731087796
70-71	0.24525163518792711
72-73	0.2511997042959929
74-75	0.25364266125109136
76-77	0.2448267731087796
78-79	0.24780080766281248
80-81	0.2448267731087796
82-83	0.2373916867236973
84-85	0.22698256578458217
86-87	0.21540507412781124
88-89	0.2062705394261388
90-91	0.21657344484546703
92-93	0.2090321429405979
94-95	0.202234349674237
96-97	0.18906362522066272
98-99	0.19894166856084344
100-101	0.19809194440254832
102-103	0.19894166856084344
104-105	0.2064829704657126
106-107	0.2000038237587123
108-109	0.20807620326251589
110-111	0.19883545304105657
112-113	0.19586141848702363
114-115	0.19766708232340077
116-117	0.19798572888276145
118-119	0.19299359945277764
120-121	0.19926031512020412
122-123	0.1932060304923514
124-125	0.21721073796418838
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	470741.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.32194935873022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.46040566068494	65.87688246068113
2	8.855996759619735	13.341018788983133
3	1.7660855192447373	3.990750121312166
4	0.6338201215640585	1.90962268395969
5	0.31974046684854995	1.204173762595162
6	0.18921290033438917	0.8551130698203199
7	0.12592112231269942	0.6639237078622134
8	0.09973034992902255	0.6009507493505823
9	0.06530705664002441	0.442714933170688
>10	0.4207760629746057	6.256897654189611
>50	0.0440701413890521	2.2680442343905733
>100	0.01828094747682771	2.066910210761778
>500	3.2644549065763764E-4	0.21662477940532074
>1k	3.2644549065763764E-4	0.3063728435176273
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1246	0.2646890753089278	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	881	0.18715174586449873	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.1243103957377835E-4	0.0
3	0.0	0.0	0.0	2.1243103957377835E-4	0.0
4	0.0	0.0	0.0	2.1243103957377835E-4	0.0
5	0.0	0.0	0.0	2.1243103957377835E-4	0.0
6	0.0	0.0	0.0	2.1243103957377835E-4	0.0
7	0.0	0.0	0.0	2.1243103957377835E-4	0.0
8	0.0	0.0	0.0	2.1243103957377835E-4	0.0
9	0.0	0.0	0.0	2.1243103957377835E-4	0.0
10-11	0.0	0.0	0.0	2.1243103957377835E-4	0.0
12-13	0.0	0.0	0.0	3.186465593606675E-4	0.0
14-15	0.0	0.0	0.0	5.310775989344459E-4	0.0
16-17	0.0	0.0	0.0	9.559396780820026E-4	0.0
18-19	0.0	0.0	0.0	0.0010621551978688918	0.0
20-21	0.0	0.0	0.0	0.0014870172770164486	0.0
22-23	0.0	0.0	0.0	0.002336741435311562	0.0
24-25	0.0	0.0	0.0	0.005416991509131348	0.0
26-27	0.0	0.0	0.0	0.00902831918188558	0.0
28-29	0.0	0.0	0.0	0.017631776284623604	0.0
30-31	0.0	0.0	0.0	0.03834380264306699	0.0
32-33	0.0	0.0	0.0	0.07254520001444531	0.0
34-35	0.0	0.0	0.0	0.10472850250987273	2.1243103957377835E-4
36-37	0.0	0.0	0.0	0.16155380559585847	3.186465593606675E-4
38-39	0.0	0.0	0.0	0.242490031673468	4.248620791475567E-4
40-41	0.0	0.0	0.0	0.3320297148538156	4.248620791475567E-4
42-43	0.0	0.0	0.0	0.4146653892480153	4.248620791475567E-4
44-45	0.0	0.0	0.0	0.49581404636519866	4.248620791475567E-4
46-47	0.0	0.0	0.0	0.5964001436033828	4.248620791475567E-4
48-49	0.0	0.0	0.0	0.7027218789100588	4.248620791475567E-4
50-51	0.0	0.0	0.0	0.8069193038209972	4.248620791475567E-4
52-53	0.0	0.0	0.0	0.8989019439564432	5.310775989344459E-4
54-55	0.0	0.0	0.0	0.993008894487627	6.372931187213351E-4
56-57	0.0	0.0	0.0	1.09157689684986	6.372931187213351E-4
58-59	0.0	0.0	0.0	1.212343942847553	6.372931187213351E-4
60-61	0.0	0.0	0.0	1.3331109888452461	6.372931187213351E-4
62-63	0.0	0.0	0.0	1.453028310684644	6.372931187213351E-4
64-65	0.0	0.0	0.0	1.5761320981176485	6.372931187213351E-4
66-67	0.0	0.0	0.0	1.7039093684212763	6.372931187213351E-4
68-69	0.0	0.0	0.0	1.8577094410726918	6.372931187213351E-4
70-71	0.0	0.0	0.0	2.017670013871747	6.372931187213351E-4
72-73	0.0	0.0	0.0	2.1684960519691296	8.497241582951134E-4
74-75	0.0	0.0	0.0	2.3148610382354624	8.497241582951134E-4
76-77	0.0	0.0	0.0	2.4696170505649606	8.497241582951134E-4
78-79	0.0	0.0	0.0	2.650714511801606	8.497241582951134E-4
80-81	0.0	0.0	0.0	2.835954378309941	8.497241582951134E-4
82-83	0.0	0.0	0.0	3.028629331203358	8.497241582951134E-4
84-85	0.0	0.0	0.0	3.211744887315955	8.497241582951134E-4
86-87	0.0	0.0	0.0	3.3856196932070928	8.497241582951134E-4
88-89	0.0	0.0	0.0	3.5874291808021823	8.497241582951134E-4
90-91	0.0	0.0	0.0	3.8064455826027475	8.497241582951134E-4
92-93	0.0	0.0	0.0	4.041925389970281	8.497241582951134E-4
94-95	0.0	0.0	0.0	4.254462645063846	8.497241582951134E-4
96-97	0.0	0.0	0.0	4.473585262384199	8.497241582951134E-4
98-99	0.0	0.0	0.0	4.717243664775323	8.497241582951134E-4
100-101	0.0	0.0	0.0	4.99181078342443	8.497241582951134E-4
102-103	0.0	0.0	0.0	5.2801859196458345	8.497241582951134E-4
104-105	0.0	0.0	0.0	5.563993788516403	8.497241582951134E-4
106-107	0.0	0.0	0.0	5.847270579788036	8.497241582951134E-4
108-109	0.0	0.0	0.0	6.138938397122834	8.497241582951134E-4
110-111	0.0	0.0	0.0	6.46034655999796	8.497241582951134E-4
112-113	0.0	0.0	0.0	6.792163843812203	8.497241582951134E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2755	0.0	30.271593	1
GGTATCA	2035	0.0	30.150997	1
TCTAACG	80	0.005328217	29.785086	2
GCCGTGT	80	0.0053309957	29.78191	8
ATGGGTA	485	0.0	29.478022	5
GGGTACC	285	0.0	29.262539	7
TGGGTAC	360	0.0	26.47563	6
CTAACGC	90	0.009468762	26.47563	3
CATGGGT	1365	0.0	25.74828	4
CATGGGG	1970	0.0	25.098093	4
TATCAAC	3315	0.0	23.899948	2
GTACATG	4945	0.0	23.852163	1
GTAAGTA	150	1.6711118E-4	23.828068	2
ATGGGGG	1085	0.0	23.608456	5
TACATGG	4950	0.0	23.587383	2
ACATGGG	4785	0.0	23.40479	3
CAACGCA	3200	0.0	23.2696	5
ATCAACG	3225	0.0	23.089212	3
AACGCAG	3235	0.0	23.01784	6
GGTACCT	390	0.0	22.909163	8
>>END_MODULE