FastQCFastQC Report
Sun 1 Jan 2017
ERR1142728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470741
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.5678281687807095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.48774166686139514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14960.3177968352023724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6270.13319426181275903No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5230.11110143369708607No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4780.10154203691626607No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT655.47152E-754.9273951
GGTATCA20900.047.54681
GTATCAA32150.039.793171
CCGCAGA3300.037.8666151
ATCAACG40850.031.5927943
TATCAAC42050.030.9740852
CAACGCA42450.030.5421165
TCAACGC42900.030.2217454
AACGCAG43950.029.4997236
TAGTACT1704.4328044E-727.9873264
ACGCAGA46900.027.5144677
CGCAGAG49000.026.3352768
GCAGAGT51550.025.1479199
CATGGGT12050.024.6776224
GAGTACT22700.024.62499812-13
CTCAACG2802.5956979E-923.3768391
ATGGGTG6700.023.0791025
CAGAGTA51750.022.23539410-11
AGTACTT23400.021.72823712-13
GTACATG41650.021.2873881
Produced by FastQC (version 0.11.5)