##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142727_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	287296
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251514117843616	33.0	33.0	33.0	27.0	33.0
2	31.365253954110045	33.0	33.0	33.0	27.0	33.0
3	31.423952996212964	33.0	33.0	33.0	27.0	33.0
4	31.373290961238585	33.0	33.0	33.0	27.0	33.0
5	31.418498691245265	33.0	33.0	33.0	27.0	33.0
6	34.73022248830475	37.0	37.0	37.0	27.0	37.0
7	34.73021204611272	37.0	37.0	37.0	27.0	37.0
8	34.69783428937402	37.0	37.0	37.0	27.0	37.0
9	34.597999972154156	37.0	37.0	37.0	27.0	37.0
10-11	34.666445060147026	37.0	37.0	37.0	27.0	37.0
12-13	34.63275158721319	37.0	37.0	37.0	27.0	37.0
14-15	34.62149316384495	37.0	37.0	37.0	27.0	37.0
16-17	34.67137899587881	37.0	37.0	37.0	27.0	37.0
18-19	34.64214085820896	37.0	37.0	37.0	27.0	37.0
20-21	34.647005179327245	37.0	37.0	37.0	27.0	37.0
22-23	34.73708822956115	37.0	37.0	37.0	27.0	37.0
24-25	34.810724827355756	37.0	37.0	37.0	27.0	37.0
26-27	34.874453525284025	37.0	37.0	37.0	27.0	37.0
28-29	34.922492829694804	37.0	37.0	37.0	27.0	37.0
30-31	34.970579123969706	37.0	37.0	37.0	27.0	37.0
32-33	34.95255938126532	37.0	37.0	37.0	27.0	37.0
34-35	34.97519631321007	37.0	37.0	37.0	27.0	37.0
36-37	34.972667562374696	37.0	37.0	37.0	27.0	37.0
38-39	34.95251935286255	37.0	37.0	37.0	27.0	37.0
40-41	34.92515558866117	37.0	37.0	37.0	27.0	37.0
42-43	34.919598602138564	37.0	37.0	37.0	27.0	37.0
44-45	34.91896858988639	37.0	37.0	37.0	27.0	37.0
46-47	34.92594571452439	37.0	37.0	37.0	27.0	37.0
48-49	34.89664492370238	37.0	37.0	37.0	27.0	37.0
50-51	34.89422755624861	37.0	37.0	37.0	27.0	37.0
52-53	34.86747814101136	37.0	37.0	37.0	27.0	37.0
54-55	34.848654349521055	37.0	37.0	37.0	27.0	37.0
56-57	34.810434186344395	37.0	37.0	37.0	27.0	37.0
58-59	34.77739857150813	37.0	37.0	37.0	27.0	37.0
60-61	34.769208412229894	37.0	37.0	37.0	27.0	37.0
62-63	34.72273021552684	37.0	37.0	37.0	27.0	37.0
64-65	34.676694071619515	37.0	37.0	37.0	27.0	37.0
66-67	34.62351720873246	37.0	37.0	37.0	27.0	37.0
68-69	34.57486703608822	37.0	37.0	37.0	27.0	37.0
70-71	34.52376468868345	37.0	37.0	37.0	27.0	37.0
72-73	34.4806941273112	37.0	37.0	37.0	27.0	37.0
74-75	34.43310731788817	37.0	37.0	37.0	27.0	37.0
76-77	34.36459087491646	37.0	37.0	37.0	27.0	37.0
78-79	34.31609211405659	37.0	37.0	37.0	27.0	37.0
80-81	34.24292193417242	37.0	37.0	37.0	27.0	37.0
82-83	34.21643879483181	37.0	37.0	37.0	27.0	37.0
84-85	34.16961948652261	37.0	37.0	37.0	27.0	37.0
86-87	34.093863123746935	37.0	37.0	37.0	27.0	37.0
88-89	34.0360499276008	37.0	37.0	37.0	27.0	37.0
90-91	33.971299635219424	37.0	37.0	37.0	27.0	37.0
92-93	33.90508395522388	37.0	37.0	37.0	22.0	37.0
94-95	33.86982937458231	37.0	37.0	37.0	22.0	37.0
96-97	33.77557988973045	37.0	35.0	37.0	22.0	37.0
98-99	33.69437792381377	37.0	33.0	37.0	22.0	37.0
100-101	33.63789262642014	37.0	33.0	37.0	22.0	37.0
102-103	33.57101212686567	37.0	33.0	37.0	22.0	37.0
104-105	33.403251350523504	37.0	33.0	37.0	22.0	37.0
106-107	33.33259251782134	37.0	33.0	37.0	22.0	37.0
108-109	33.22801918578748	37.0	33.0	37.0	18.0	37.0
110-111	33.14206950323012	37.0	33.0	37.0	14.0	37.0
112-113	33.04411478057474	37.0	33.0	37.0	14.0	37.0
114-115	32.919104338382716	37.0	33.0	37.0	14.0	37.0
116-117	32.82049697872577	37.0	33.0	37.0	14.0	37.0
118-119	32.692910795834266	37.0	33.0	37.0	14.0	37.0
120-121	32.552489418578745	37.0	33.0	37.0	14.0	37.0
122-123	32.43615991869014	37.0	33.0	37.0	14.0	37.0
124-125	30.81857213466251	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	528.0
1	24.0
2	16.0
3	15.0
4	8.0
5	7.0
6	10.0
7	7.0
8	9.0
9	9.0
10	11.0
11	8.0
12	13.0
13	10.0
14	30.0
15	124.0
16	185.0
17	288.0
18	575.0
19	1111.0
20	1833.0
21	2486.0
22	2612.0
23	2714.0
24	2684.0
25	3120.0
26	3503.0
27	4343.0
28	5135.0
29	6269.0
30	7390.0
31	8993.0
32	11400.0
33	15363.0
34	22949.0
35	44151.0
36	139363.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.533847711482736	20.063540830220298	12.27420312118144	24.128408337115527
2	17.76567004767855	22.682564170581706	35.89294315571968	23.65882262602006
3	19.68435233594628	27.50678830944919	28.221916641654044	24.58694271295049
4	14.57822115334271	19.50127230015812	33.83061827853774	32.08988826796143
5	15.366355159762199	37.61821145931152	30.93937309962752	16.076060281298766
6	29.33596929063176	36.31648253387217	18.07842904396483	16.269119131531244
7	26.08460267618572	33.86307513368704	21.128664387030526	18.92365780309672
8	25.733478183412338	33.90749389568698	19.914207766712195	20.444820154188484
9	26.579952917507843	18.19766264067117	19.709331973482914	35.513052468338074
10-11	25.418157748122066	27.473798009329848	24.80478403551699	22.30326020703109
12-13	26.23891989136035	26.349131494144462	24.668710209856165	22.74323840463902
14-15	24.699217064945362	26.627126961922077	22.98123086542005	25.692425107712513
16-17	24.07295575530151	28.207173400820317	23.639235535387034	24.080635308491143
18-19	24.070848662119808	27.326368770462622	25.379060237767803	23.22372232964977
20-21	26.207742534383538	25.87184650336594	24.68006993617103	23.24034102607949
22-23	26.427640452182832	25.53866393547025	24.373834254297883	23.659861358049035
24-25	24.912069506961167	26.291566349139888	24.828151714993545	23.968212428905407
26-27	24.299104556780033	26.597909003985176	24.981679357346753	24.121307081888038
28-29	24.146503148823335	26.393943094394924	25.33974146503149	24.119812291750257
30-31	24.73918717114904	26.096460621624264	25.159453461919583	24.004898745307113
32-33	24.314252316066785	26.65142976778268	24.546120697175358	24.488197218975174
34-35	24.725973507137862	26.527578849673155	24.944741116273065	23.80170652691592
36-37	24.349312678808175	26.753017933151906	24.716872514130205	24.180796873909706
38-39	24.28324197583417	26.67313321912192	24.895412818428277	24.14821198661563
40-41	24.14337445228992	26.66598057547933	25.157161396556056	24.033483575674694
42-43	24.29514934355822	26.769148108477747	24.915480721928958	24.020221826035076
44-45	24.154008777376973	27.017471026981017	24.69631531575532	24.13220487988669
46-47	24.296590879359513	26.637014555698002	24.627996825479883	24.4383977394626
48-49	24.32369728432276	26.72503419018114	24.897778335984817	24.05349018951129
50-51	23.959283714357984	27.081214510628275	24.830828543267593	24.12867323174614
52-53	23.79840106618846	26.930329677614058	24.74701573639746	24.52425351980002
54-55	23.82110360611812	26.67955230545942	24.94190995869153	24.557434129730936
56-57	24.210845811412383	26.414860659512414	24.515762151299906	24.858531377775297
58-59	24.102778795332043	26.725975543810073	24.831754670574075	24.33949099028381
60-61	24.073066147506953	26.63816172802032	25.085916048245565	24.20285607622716
62-63	23.958942081749495	26.705614935480664	24.739593782000178	24.595849200769663
64-65	23.714115249884863	26.412187905600604	25.047450909243157	24.826245935271377
66-67	24.25256531274918	26.25648416494075	24.86621638833976	24.624734133970307
68-69	24.331143067512066	26.144480774397966	24.735405885452472	24.7889702726375
70-71	24.0895180735504	26.255761936499184	24.876386615906956	24.778333374043456
72-73	24.38049839301231	26.1710421166872	25.175093610086506	24.273365880213984
74-75	24.427193207456305	26.083277060333383	25.15586312312532	24.333666609084993
76-77	24.281347486548167	25.933253773841674	25.07554662256	24.709852117050158
78-79	24.07715969145894	26.113628393314826	25.104641201502574	24.704570713723655
80-81	24.02565453612816	26.123868977622074	25.38916242406055	24.461314062189217
82-83	24.497657070483275	25.834853998681123	25.5906171168185	24.076871814017103
84-85	24.234283581100176	26.26601820484462	25.04003460944014	24.459663604615056
86-87	23.908595708163492	26.424114937356208	25.16286687562465	24.504422478855652
88-89	24.32163748809661	25.74394017085073	25.225597611298888	24.70882472975377
90-91	24.27719249284867	25.876473871485384	25.235121747017374	24.611211888648572
92-93	24.27389691455241	26.11581721369659	25.230697857749547	24.379588014001456
94-95	23.29386117893268	26.30327868852459	25.587547959539584	24.81531217300314
96-97	23.55395751195772	26.273150368191338	25.271544380875323	24.90134773897562
98-99	23.366746537478246	26.74573528232065	25.13820440649709	24.749313773704017
100-101	23.653545668884888	26.936759720420206	24.930593339750835	24.47910127094407
102-103	23.789809250429876	27.00175437984591	25.10158312726055	24.106853242463664
104-105	23.62950147200402	27.283873533228224	25.07796040239427	24.008664592373485
106-107	23.65238864222161	27.037853448245798	25.05990352654764	24.249854382984957
108-109	23.558117429560575	27.288202105116287	24.90438028132167	24.249300184001466
110-111	23.43120021205653	27.946295288717753	24.57710766054214	24.045396838683576
112-113	23.478626308281978	27.821895783853158	24.47694877777429	24.22252913009057
114-115	23.37139628485132	27.67231216735608	24.78951443578708	24.166777112005526
116-117	23.631636037682544	27.670546299235117	24.557306835105734	24.140510827976602
118-119	23.293174791665578	27.395170163755477	24.798902135569517	24.51275290900943
120-121	23.25234642307813	27.519505568286057	25.143959290300756	24.08418871833506
122-123	23.583900505528728	27.63632622384512	25.02218990372459	23.757583366901557
124-125	23.545397144342232	27.446547989074126	24.774906770762474	24.233148095821168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	831.0
1	508.5
2	144.0
3	89.0
4	73.0
5	59.0
6	48.0
7	52.0
8	65.0
9	60.5
10	51.0
11	49.5
12	50.0
13	51.0
14	50.5
15	64.0
16	84.5
17	104.0
18	116.0
19	140.0
20	172.0
21	205.0
22	269.5
23	327.0
24	394.5
25	493.5
26	592.0
27	717.0
28	906.0
29	1259.5
30	1812.5
31	2290.5
32	2762.0
33	3338.5
34	3927.5
35	4515.0
36	4948.5
37	5711.0
38	6222.0
39	6207.0
40	6292.5
41	6322.0
42	6469.5
43	7090.0
44	8198.0
45	9246.0
46	10400.0
47	11617.0
48	12376.0
49	12646.5
50	12466.0
51	12376.5
52	12562.0
53	12758.5
54	12533.5
55	11920.0
56	11169.0
57	9910.5
58	8845.5
59	8015.5
60	6942.0
61	5736.5
62	4639.0
63	3659.5
64	2778.0
65	2158.5
66	1640.0
67	1224.5
68	1004.0
69	863.0
70	725.5
71	656.5
72	573.5
73	529.5
74	435.0
75	341.5
76	265.5
77	182.5
78	125.0
79	85.5
80	61.0
81	42.5
82	29.5
83	18.0
84	10.0
85	5.0
86	3.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3014312764535531
2	0.27637001559367347
3	0.26940855424370685
4	0.2808949654711517
5	0.2920333036310982
6	0.3477249944308309
7	0.3456365560258409
8	0.3560787480507908
9	0.34459233682334595
10-11	0.3517278347070617
12-13	0.35799314992203163
14-15	0.3485951770995767
16-17	0.28576798841612827
18-19	0.27776230786366674
20-21	0.26070672755624863
22-23	0.2542673758075295
24-25	0.24573958565382042
26-27	0.2554856315437737
28-29	0.2370377589663622
30-31	0.24086656270884385
32-33	0.2474799509913121
34-35	0.24034445310759633
36-37	0.2352973936288706
38-39	0.24121463577634217
40-41	0.22555134773891736
42-43	0.23494932056137224
44-45	0.22624749387391402
46-47	0.22224465359768322
48-49	0.23112051681889062
50-51	0.23616757629761642
52-53	0.2323387725551348
54-55	0.23390510135887724
56-57	0.22972822454889733
58-59	0.23007629761639561
60-61	0.23634161283136554
62-63	0.23547143016261976
64-65	0.2380819781688572
66-67	0.25496352194252614
68-69	0.2523529739362887
70-71	0.24956838939630208
72-73	0.255833704611272
74-75	0.2598365448875028
76-77	0.2499164624638004
78-79	0.25113471820004457
80-81	0.25026453553129874
82-83	0.23982234350634885
84-85	0.2328608821563823
86-87	0.22120043439518822
88-89	0.21336879037647583
90-91	0.2213744709289374
92-93	0.21284668077522834
94-95	0.20745154822900425
96-97	0.19370266206282025
98-99	0.20292659835152593
100-101	0.20188237914903098
102-103	0.20362274448652262
104-105	0.2130207173089775
106-107	0.20362274448652262
108-109	0.213542826910225
110-111	0.20188237914903098
112-113	0.196661283136556
114-115	0.20118623301403432
116-117	0.20362274448652262
118-119	0.19405073513031856
120-121	0.20292659835152593
122-123	0.19718339273780353
124-125	0.22172254399643573
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	287296.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.94248567610313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.41428661447993	58.886732314533496
2	9.423990944720366	12.994267214362187
3	2.244003991415801	4.641216391059063
4	0.8705067878039378	2.4005960699649407
5	0.4375656542708256	1.5083431925960546
6	0.27227554520414976	1.126281172511416
7	0.18495270195605032	0.8925769303753097
8	0.1467957395342475	0.8096370536122258
9	0.1157915829644172	0.7184663594943875
>10	0.8038184530728725	10.87751986016667
>50	0.06478824812323081	2.8913940928820128
>100	0.020106697693416456	1.8344122331189119
>500	0.0011170387607453587	0.4185571153233118
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	569	0.19805357540654936	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	518	0.18030184896413454	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4807306749832926E-4
2	0.0	0.0	0.0	0.0	3.4807306749832926E-4
3	0.0	0.0	0.0	0.0	3.4807306749832926E-4
4	0.0	0.0	0.0	0.0	3.4807306749832926E-4
5	0.0	0.0	0.0	0.0	3.4807306749832926E-4
6	0.0	0.0	0.0	0.0	6.961461349966585E-4
7	0.0	0.0	0.0	0.0	6.961461349966585E-4
8	0.0	0.0	0.0	0.0	6.961461349966585E-4
9	0.0	0.0	0.0	0.0	6.961461349966585E-4
10-11	0.0	0.0	0.0	0.0	6.961461349966585E-4
12-13	0.0	0.0	0.0	0.0	6.961461349966585E-4
14-15	0.0	0.0	0.0	3.4807306749832926E-4	6.961461349966585E-4
16-17	0.0	0.0	0.0	3.4807306749832926E-4	6.961461349966585E-4
18-19	0.0	0.0	0.0	6.961461349966585E-4	6.961461349966585E-4
20-21	0.0	0.0	0.0	0.0015663288037424815	6.961461349966585E-4
22-23	0.0	0.0	0.0	0.003132657607484963	6.961461349966585E-4
24-25	0.0	0.0	0.0	0.00678742481621742	0.0010442192024949877
26-27	0.0	0.0	0.0	0.012704666963689017	0.0010442192024949877
28-29	0.0	0.0	0.0	0.026627589663622185	0.0010442192024949877
30-31	0.0	0.0	0.0	0.050644631321006904	0.0010442192024949877
32-33	0.0	0.0	0.0	0.08684423034083315	0.0010442192024949877
34-35	0.0	0.0	0.0	0.1298312541768768	0.0010442192024949877
36-37	0.0	0.0	0.0	0.1914401871240811	0.0010442192024949877
38-39	0.0	0.0	0.0	0.284375696146135	0.0010442192024949877
40-41	0.0	0.0	0.0	0.3743525840944531	0.0010442192024949877
42-43	0.0	0.0	0.0	0.4437931610603698	0.0010442192024949877
44-45	0.0	0.0	0.0	0.5248941857874805	0.0010442192024949877
46-47	0.0	0.0	0.0	0.6218325350857652	0.0010442192024949877
48-49	0.0	0.0	0.0	0.7109392403653374	0.0010442192024949877
50-51	0.0	0.0	0.0	0.8054410781911339	0.0010442192024949877
52-53	0.0	0.0	0.0	0.8971583314769436	0.0010442192024949877
54-55	0.0	0.0	0.0	0.9895717308977501	0.0010442192024949877
56-57	0.0	0.0	0.0	1.0892946647360215	0.0010442192024949877
58-59	0.0	0.0	0.0	1.2119904210291825	0.0010442192024949877
60-61	0.0	0.0	0.0	1.3285948986411227	0.0010442192024949877
62-63	0.0	0.0	0.0	1.4335389284918691	0.0010442192024949877
64-65	0.0	0.0	0.0	1.5350022276676318	0.0010442192024949877
66-67	0.0	0.0	0.0	1.643949097794609	0.001392292269993317
68-69	0.0	0.0	0.0	1.7563766985965694	0.001392292269993317
70-71	0.0	0.0	0.0	1.8896886834484294	0.001392292269993317
72-73	0.0	0.0	0.0	2.0183016818890622	0.001392292269993317
74-75	0.0	0.0	0.0	2.1335138672310094	0.001392292269993317
76-77	0.0	0.0	0.0	2.2589942080641565	0.001392292269993317
78-79	0.0	0.0	0.0	2.4107540654934283	0.001392292269993317
80-81	0.0	0.0	0.0	2.5640802517264425	0.001392292269993317
82-83	0.0	0.0	0.0	2.711315159278236	0.001392292269993317
84-85	0.0	0.0	0.0	2.865859601247494	0.001392292269993317
86-87	0.0	0.0	0.0	3.025625139229227	0.001392292269993317
88-89	0.0	0.0	0.0	3.2008799287146354	0.001392292269993317
90-91	0.0	0.0	0.0	3.38744709289374	0.001392292269993317
92-93	0.0	0.0	0.0	3.595246714190243	0.001392292269993317
94-95	0.0	0.0	0.0	3.7861647917130767	0.001392292269993317
96-97	0.0	0.0	0.0	3.9795193807083984	0.001392292269993317
98-99	0.0	0.0	0.0	4.18540460013366	0.001392292269993317
100-101	0.0	0.0	0.0	4.422268322566273	0.001392292269993317
102-103	0.0	0.0	0.0	4.6549551681889065	0.001392292269993317
104-105	0.0	0.0	0.0	4.904697594118957	0.001392292269993317
106-107	0.0	0.0	0.0	5.157572677656494	0.001392292269993317
108-109	0.0	0.0	0.0	5.405922811316552	0.001392292269993317
110-111	0.0	0.0	0.0	5.673416963689018	0.001392292269993317
112-113	0.0	0.0	0.0	5.951701381153931	0.001392292269993317
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	775	0.0	49.20524	1
GTATCAA	1115	0.0	34.20095	2
ACGCAAG	75	0.0038752647	31.783943	8
GAGTTGA	105	5.8400165E-4	28.37852	9
GGGTAAA	130	6.300622E-5	27.500525	1
CAACGCA	1370	0.0	26.534954	6
ATCAACG	1370	0.0	26.530312	4
TATCAAC	1505	0.0	26.525976	3
TAAAACG	90	0.009451632	26.481987	4
AACGCAG	1355	0.0	26.388884	7
TATTTAG	185	9.1048605E-7	25.766258	2
TCAACGC	1440	0.0	25.240644	5
CGCAGAG	1595	0.0	22.791779	9
ACGCAGA	1570	0.0	22.775118	8
ATCCTAG	160	2.5797356E-4	22.344175	1
GTACATG	2175	0.0	21.368225	1
TACATGG	2265	0.0	21.308353	2
GTGGTAT	365	1.09139364E-10	21.221865	1
GGTAAAA	170	3.886267E-4	21.029814	2
TTAAACA	145	0.003828787	20.54637	4
>>END_MODULE