FastQCFastQC Report
Sun 1 Jan 2017
ERR1142727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287296
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.5906799955446647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16730.5823262419247048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9180.31953107596346625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.1642904878592114No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.078.4371951
GTATCAA15700.051.176131
GTGGTAT2300.046.577691
GTGTTAT1053.2455137E-739.677291
TATCAAC21450.037.438022
CAACGCA22450.036.56535
ATCAACG22200.036.173223
CCGCAGA2800.036.1346781
AACGCAG24000.034.4516456
TCAACGC23800.034.241284
TGTTATC902.3921196E-433.0471422
GTGTATA902.3945665E-433.041399
TGGTATC3250.032.9454572
ACGCAGA26200.031.7802667
CGCAGAG27250.030.5557088
TCACCGT800.005362258329.7424286
TAAGTGT1004.43705E-429.7424286
GCAGAGT28750.028.754639
CATGGGG12800.028.3482514
TTAAGAT1055.903562E-428.326123
Produced by FastQC (version 0.11.5)