FastQCFastQC Report
Sun 1 Jan 2017
ERR1142726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446115
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12830.28759400602983537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8260.18515405220626968No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.037.306561
GTATCAA21450.030.8066371
GACGTTA800.005338889529.7725647
CGTTAAC850.007179073528.0212389
ACGTTAA900.009487675526.4645028
TATCAAC26300.025.1255662
CATGGGG20550.024.6238374
ATCAACG25700.024.5539473
CAACGCA26250.023.81275
AACGCAG26800.023.7736176
TCAACGC26700.023.6343254
GTACATG40400.023.4295731
TACATGG40550.023.1960932
ACATGGG39650.023.122043
GTGTTAC1350.0025449522.0487961
TGGGGGA6500.021.9858916
ATGGGGG12550.021.82045
ACGCAGA29050.021.3173627
CGCAGAG29650.020.8859838
GTCCTAC1500.00468696519.8439141
Produced by FastQC (version 0.11.5)