FastQCFastQC Report
Sun 1 Jan 2017
ERR1142726_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142726_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446115
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25420.5698082333030721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24200.5424610246236957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.3055266018851641No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.1304596348475169No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.11050962195846363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.049.8166771
GTATCAA29400.037.8473051
GTCGACG650.00194137936.6009679
CCGCAGA3000.035.7019541
ATCAACG36950.030.7443183
TATCAAC37600.030.3710172
CAACGCA38950.029.6237625
TCAACGC39350.029.3226344
AACGCAG41100.028.07416
AGTCGAC900.00954112926.4340328
ACGCAGA44250.026.0756057
CGCAGAG45350.025.3119728
GCAGAGT48100.023.8648229
CTCAACG3103.3469405E-1023.0335161
GAGTACT22750.022.74488812-13
ATGGGAG4250.022.3911825
CAGAGTA48600.022.0895510-11
CATGGGT4600.021.9804744
GTACTAG1400.003151670821.2511621
GTAAGTA2004.9153692E-520.8168032
Produced by FastQC (version 0.11.5)