##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446115
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.54159129372471	14.0	14.0	33.0	14.0	33.0
2	27.263938670522176	27.0	27.0	33.0	14.0	33.0
3	31.01323201416675	33.0	27.0	33.0	27.0	33.0
4	30.500848435941407	33.0	33.0	33.0	27.0	33.0
5	32.230924761552515	33.0	33.0	33.0	33.0	33.0
6	34.78130078567185	37.0	37.0	37.0	27.0	37.0
7	34.98177375788754	37.0	37.0	37.0	33.0	37.0
8	35.341981327684564	37.0	37.0	37.0	33.0	37.0
9	35.85940844849422	37.0	37.0	37.0	33.0	37.0
10-11	35.97332862602692	37.0	37.0	37.0	33.0	37.0
12-13	36.05766450354729	37.0	37.0	37.0	35.0	37.0
14-15	36.055116954148595	37.0	37.0	37.0	37.0	37.0
16-17	36.06430404716273	37.0	37.0	37.0	37.0	37.0
18-19	36.07314705849389	37.0	37.0	37.0	37.0	37.0
20-21	36.08966297927664	37.0	37.0	37.0	37.0	37.0
22-23	36.07867814352801	37.0	37.0	37.0	37.0	37.0
24-25	36.06434999943961	37.0	37.0	37.0	37.0	37.0
26-27	35.96007419611535	37.0	37.0	37.0	35.0	37.0
28-29	35.92994407271668	37.0	37.0	37.0	33.0	37.0
30-31	35.93747127982695	37.0	37.0	37.0	33.0	37.0
32-33	35.884807728948815	37.0	37.0	37.0	33.0	37.0
34-35	35.81319839054952	37.0	37.0	37.0	33.0	37.0
36-37	35.74473622272284	37.0	37.0	37.0	33.0	37.0
38-39	35.684481579861696	37.0	37.0	37.0	33.0	37.0
40-41	35.60616432982527	37.0	37.0	37.0	33.0	37.0
42-43	35.519974670208356	37.0	37.0	37.0	33.0	37.0
44-45	35.402408571780825	37.0	37.0	37.0	33.0	37.0
46-47	35.262753998408485	37.0	37.0	37.0	33.0	37.0
48-49	35.12989027492911	37.0	37.0	37.0	33.0	37.0
50-51	35.03473543817177	37.0	37.0	37.0	33.0	37.0
52-53	34.95691918003206	37.0	37.0	37.0	27.0	37.0
54-55	34.8849523105029	37.0	37.0	37.0	27.0	37.0
56-57	34.83875458121785	37.0	37.0	37.0	27.0	37.0
58-59	34.78395032670948	37.0	37.0	37.0	27.0	37.0
60-61	34.745266355087814	37.0	37.0	37.0	27.0	37.0
62-63	34.72212321934927	37.0	37.0	37.0	27.0	37.0
64-65	34.69970523295562	37.0	37.0	37.0	27.0	37.0
66-67	34.690259238088835	37.0	37.0	37.0	27.0	37.0
68-69	34.64613384441232	37.0	37.0	37.0	27.0	37.0
70-71	34.60081705389865	37.0	37.0	37.0	27.0	37.0
72-73	34.56857424655078	37.0	37.0	37.0	27.0	37.0
74-75	34.47435862950136	37.0	37.0	37.0	27.0	37.0
76-77	34.40370644340585	37.0	37.0	37.0	27.0	37.0
78-79	34.40081481232418	37.0	37.0	37.0	27.0	37.0
80-81	34.36825258061262	37.0	37.0	37.0	27.0	37.0
82-83	34.3266747363348	37.0	37.0	37.0	27.0	37.0
84-85	34.21895139145736	37.0	37.0	37.0	27.0	37.0
86-87	34.10422760947289	37.0	37.0	37.0	27.0	37.0
88-89	34.034562836936665	37.0	37.0	37.0	27.0	37.0
90-91	33.97411093552111	37.0	37.0	37.0	27.0	37.0
92-93	33.87296100781189	37.0	37.0	37.0	24.5	37.0
94-95	33.904111047599834	37.0	37.0	37.0	24.5	37.0
96-97	33.881046366968164	37.0	37.0	37.0	22.0	37.0
98-99	33.81888974815911	37.0	37.0	37.0	22.0	37.0
100-101	33.72947110050099	37.0	33.0	37.0	22.0	37.0
102-103	33.63148067202403	37.0	33.0	37.0	22.0	37.0
104-105	33.53161628728019	37.0	33.0	37.0	22.0	37.0
106-107	33.440054694417356	37.0	33.0	37.0	18.0	37.0
108-109	33.32034340921063	37.0	33.0	37.0	14.0	37.0
110-111	33.27273124642749	37.0	33.0	37.0	14.0	37.0
112-113	33.20218329354539	37.0	33.0	37.0	14.0	37.0
114-115	33.09139795792565	37.0	33.0	37.0	14.0	37.0
116-117	33.00099750064445	37.0	33.0	37.0	14.0	37.0
118-119	32.894547370072736	37.0	33.0	37.0	14.0	37.0
120-121	32.8000874214048	37.0	33.0	37.0	14.0	37.0
122-123	32.68013404615402	37.0	33.0	37.0	14.0	37.0
124-125	31.438240139874246	37.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	7.0
16	14.0
17	6.0
18	83.0
19	317.0
20	1246.0
21	2820.0
22	4395.0
23	5833.0
24	4833.0
25	3753.0
26	3585.0
27	4294.0
28	5642.0
29	7153.0
30	9551.0
31	13066.0
32	18533.0
33	27700.0
34	46147.0
35	94641.0
36	192496.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.56189391153015	13.623478331075686	13.159688893274604	29.65493886411956
2	17.109713863017383	20.587292514262018	39.00474093002925	23.298252692691346
3	19.883662284388553	26.694686347690617	29.31172455532766	24.109926812593166
4	14.057200754069035	18.909110472010454	34.83646520052094	32.19722357339956
5	15.658772234613702	35.370044497248344	33.02502830114662	15.946154966991335
6	29.035981995552685	35.22702819023026	19.16962556488057	16.56736424933649
7	25.581296302522894	32.5434024859061	23.109960436210393	18.765340775360613
8	25.54251706398574	32.204252266792196	21.67086961882026	20.5823610504018
9	26.5028075720386	16.17206325723188	21.41577844277821	35.90935072795131
10-11	24.968673996615223	25.559216793876015	27.2828754917454	22.189233717763358
12-13	26.165226455062037	24.593434428342466	27.285677459847797	21.9556616567477
14-15	24.27625163915134	24.70293534178407	26.291090862221623	24.729722156842968
16-17	22.824944240834764	26.926577227844838	27.006713515573338	23.24176501574706
18-19	22.62107304170449	25.49376281900407	29.74703831971577	22.13812581957567
20-21	23.9915895277067	24.406284989929624	29.65464834385203	21.947477138511648
22-23	23.955146094616858	24.282191811528417	29.53565784607108	22.227004247783643
24-25	23.02803088889636	24.98683074991874	29.61063851249117	22.37449984869372
26-27	22.656601997242866	25.140490680654093	29.694249240666643	22.5086580814364
28-29	22.47839682593053	25.049594835412393	30.044607332190132	22.427401006466944
30-31	22.875155509229682	24.743395761182654	29.968057563632694	22.413391165954966
32-33	22.67108556211466	25.035164739097247	29.67847940383018	22.61527029495791
34-35	22.7865012384699	24.895262432332473	29.806440043486543	22.511796285711082
36-37	22.894801730514896	25.068705027907917	29.484207930779405	22.552285310797785
38-39	22.75933258837757	25.000028019900856	29.564357789476396	22.67628160224518
40-41	22.47574235619423	24.994591977296395	29.712069025423872	22.817596641085505
42-43	22.751166194808892	24.961276280012285	29.348769022817294	22.938788502361533
44-45	22.836850180839225	25.13244918064757	29.113153685881095	22.917546952632115
46-47	22.92438376246457	25.048726279912444	28.8654594280168	23.16143052960619
48-49	22.91158109456082	25.335059345684407	28.722750860204204	23.030608699550566
50-51	22.774957129888033	25.654932024253835	28.50610268652702	23.064008159331113
52-53	22.7171245082546	25.427300135615255	28.39357564753483	23.461999708595318
54-55	22.70944198119763	25.48294830469342	28.263515603634946	23.544094110474003
56-57	22.935229705345034	25.40914338231174	27.997489436580253	23.658137475762974
58-59	22.695110975619322	25.4529416973111	28.34784577398251	23.504101553087065
60-61	22.862628316273064	25.54276984944448	28.403246251803065	23.191355582479385
62-63	22.532867085841094	25.48838304024747	28.496575994978873	23.482173878932564
64-65	22.511011734642413	25.29336606032077	28.562142048574916	23.6334801564619
66-67	22.823400718873742	25.229173227949325	28.44908650133542	23.498339551841514
68-69	22.678681505889735	25.25279356219809	28.591842910460308	23.476682021451868
70-71	22.584600486649638	25.112780100938892	28.803337282271364	23.499282130140113
72-73	22.790337458208597	25.272659821637717	28.914886200739943	23.02211651941374
74-75	22.78262215571952	25.283278334002595	28.976159977387088	22.957939532890798
76-77	22.609505221478166	25.17010465390181	28.88503774494958	23.335352379670447
78-79	22.349134404372027	25.454429298669158	28.853486479761674	23.34294981719714
80-81	22.442643712943973	25.52099794895935	28.824294184235004	23.21206415386167
82-83	22.58004137965045	25.202807360466966	29.121209200359548	23.095942059523036
84-85	22.57100803475069	25.399886467918215	28.75906742145308	23.270038075878023
86-87	22.3549447823007	25.48580012057296	28.792575220573298	23.36667987655305
88-89	22.5169831959292	25.267424270500282	28.736499636696056	23.479092896874462
90-91	22.67912423168379	25.22006370855579	28.64675400421733	23.454058055543094
92-93	22.52238501096523	25.486140260696434	28.56381271338432	23.427662014954016
94-95	22.16981819363762	25.461857202259996	28.6818282181497	23.686496385952683
96-97	22.321822848368694	25.417885522791206	28.379341649574663	23.880949979265438
98-99	22.33112538246865	25.52548109792318	28.25706376158614	23.886329758022036
100-101	22.488483911099156	25.793685484684442	27.891911278482006	23.825919325734397
102-103	22.502818792548076	25.720165070284885	28.07282011508295	23.70419602208409
104-105	22.459257100585084	25.95586092492546	27.832500168127506	23.752381806361946
106-107	22.397500030823686	25.81556674673353	27.837824811611227	23.949108410831556
108-109	22.507916402786574	25.96378350810135	27.52688774680962	24.001412342302455
110-111	22.309710697717165	26.28558554822396	27.578372549964637	23.826331204094238
112-113	22.2249745287111	26.210040339348183	27.461131765863655	24.103853366077065
114-115	22.24841234081377	26.292600039901636	27.319842993788345	24.139144625496247
116-117	22.26583339684475	26.337550491578387	27.408240942898004	23.988375168678857
118-119	22.15455104833657	26.061758103075128	27.57752265969382	24.20616818889448
120-121	22.025262544411195	26.16264864440783	27.709783351826324	24.10230545935465
122-123	22.03927238492317	26.335810273135852	27.72581060937202	23.899106732568956
124-125	22.098275328727635	26.203279879134033	27.508215388891628	24.190229403246704
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	173.0
2	298.0
3	395.5
4	291.5
5	254.0
6	359.5
7	786.0
8	1349.0
9	1587.5
10	1352.5
11	1006.5
12	877.5
13	845.0
14	851.5
15	831.0
16	825.0
17	825.5
18	834.5
19	833.5
20	803.5
21	804.0
22	859.5
23	932.5
24	1015.0
25	1125.0
26	1325.0
27	1603.0
28	1952.0
29	2523.0
30	3333.0
31	4179.5
32	5111.0
33	5917.5
34	6705.5
35	7659.0
36	8653.0
37	9841.0
38	10791.5
39	11299.0
40	11584.5
41	11698.5
42	11906.5
43	12753.0
44	14077.0
45	15284.0
46	16744.5
47	17953.5
48	18799.5
49	19213.0
50	18857.0
51	18284.5
52	17451.0
53	16808.0
54	16299.5
55	15387.0
56	14193.0
57	12544.0
58	11300.5
59	10335.5
60	8766.5
61	7235.5
62	5954.5
63	4709.0
64	3529.5
65	2668.0
66	2033.5
67	1537.0
68	1243.5
69	1100.5
70	945.5
71	796.5
72	673.5
73	603.5
74	482.0
75	365.0
76	303.5
77	209.5
78	136.5
79	93.0
80	66.0
81	35.5
82	18.5
83	14.0
84	8.0
85	6.5
86	5.0
87	1.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04998711094672899
2	0.0
3	0.0
4	4.4831489638321955E-4
5	0.004483148963832196
6	6.724723445748294E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.001008708516862244
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.1207872409580489E-4
34-35	0.0
36-37	0.001120787240958049
38-39	7.845510686706343E-4
40-41	0.004146912791544781
42-43	0.0013449446891496588
44-45	1.1207872409580489E-4
46-47	1.1207872409580489E-4
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.2415744819160978E-4
56-57	0.0
58-59	7.845510686706343E-4
60-61	1.1207872409580489E-4
62-63	0.0
64-65	0.0
66-67	1.1207872409580489E-4
68-69	0.0
70-71	7.845510686706343E-4
72-73	1.1207872409580489E-4
74-75	0.07890342176344664
76-77	0.08069668134897953
78-79	0.0013449446891496588
80-81	0.0
82-83	4.4831489638321955E-4
84-85	0.09549107292962578
86-87	0.16621274783407863
88-89	0.20073299485558657
90-91	0.07509274514418927
92-93	0.13808098808603164
94-95	0.0019053383096286833
96-97	0.0
98-99	0.0
100-101	0.0
102-103	4.4831489638321955E-4
104-105	0.005603936204790244
106-107	0.006612644721652489
108-109	0.010647478789101465
110-111	0.00549185748069444
112-113	0.00549185748069444
114-115	0.004034834067448976
116-117	2.2415744819160978E-4
118-119	7.845510686706343E-4
120-121	0.0
122-123	0.0
124-125	2.2415744819160978E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	446115.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.94468705171026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.7136695285458	64.12015156393743
2	9.085099902854035	13.435897383001297
3	1.9775565408123381	4.386893986122931
4	0.7422130483240896	2.195308463360828
5	0.38935776526623306	1.4395469051882432
6	0.22948724261347553	1.0181617402448013
7	0.1575635553142949	0.8155691451929262
8	0.10233561019114631	0.6053739735864101
9	0.07934485242674116	0.5280417253673557
>10	0.4771376996823073	6.51993420720382
>50	0.03198158922109003	1.6093959066791361
>100	0.012862160870558276	1.5502741904229613
>500	3.4762596947454796E-4	0.14960364430876527
>1k	0.0010428779084236438	1.625847165383059
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2542	0.5698082333030721	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2420	0.5424610246236957	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.3055266018851641	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.1304596348475169	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	493	0.11050962195846363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.2415744819160978E-4	0.0
2	0.0	0.0	0.0	2.2415744819160978E-4	0.0
3	0.0	0.0	0.0	2.2415744819160978E-4	0.0
4	0.0	0.0	0.0	2.2415744819160978E-4	0.0
5	0.0	0.0	0.0	2.2415744819160978E-4	0.0
6	0.0	0.0	0.0	2.2415744819160978E-4	0.0
7	0.0	0.0	0.0	2.2415744819160978E-4	0.0
8	0.0	0.0	0.0	2.2415744819160978E-4	0.0
9	0.0	0.0	0.0	2.2415744819160978E-4	0.0
10-11	0.0	0.0	0.0	2.2415744819160978E-4	0.0
12-13	0.0	0.0	0.0	2.2415744819160978E-4	0.0
14-15	0.0	0.0	0.0	4.4831489638321955E-4	0.0
16-17	0.0	0.0	0.0	4.4831489638321955E-4	0.0
18-19	0.0	0.0	0.0	8.966297927664392E-4	0.0
20-21	0.0	0.0	0.0	0.0014570234132454635	0.0
22-23	0.0	0.0	0.0	0.003026125550586732	0.0
24-25	0.0	0.0	0.0	0.0059401723770776595	0.0
26-27	0.0	0.0	0.0	0.012104502202346927	0.0
28-29	0.0	0.0	0.0	0.023536532060119027	0.0
30-31	0.0	0.0	0.0	0.04371070239736391	0.0
32-33	0.0	0.0	0.0	0.07453235152371027	0.0
34-35	0.0	0.0	0.0	0.10479360702957757	0.0
36-37	0.0	0.0	0.0	0.15298745839077368	0.0
38-39	0.0	0.0	0.0	0.22819228225905874	0.0
40-41	0.0	0.0	0.0	0.30586283805745157	0.0
42-43	0.0	0.0	0.0	0.3788260874438205	0.0
44-45	0.0	0.0	0.0	0.453246360243435	0.0
46-47	0.0	0.0	0.0	0.5374174820393844	0.0
48-49	0.0	0.0	0.0	0.6288737209015612	0.0
50-51	0.0	0.0	0.0	0.7127086065252233	0.0
52-53	0.0	0.0	0.0	0.7931811304260112	0.0
54-55	0.0	0.0	0.0	0.8725328670858411	0.0
56-57	0.0	0.0	0.0	0.962195846362485	0.0
58-59	0.0	0.0	0.0	1.0626183831523264	0.0
60-61	0.0	0.0	0.0	1.1729038476625981	0.0
62-63	0.0	0.0	0.0	1.2732143057283436	0.0
64-65	0.0	0.0	0.0	1.3683691424856819	0.0
66-67	0.0	0.0	0.0	1.472266119722493	0.0
68-69	0.0	0.0	0.0	1.6018291247772436	0.0
70-71	0.0	0.0	0.0	1.7360994362440176	0.0
72-73	0.0	0.0	0.0	1.8598343476457864	0.0
74-75	0.0	0.0	0.0	1.9729217802584536	0.0
76-77	0.0	0.0	0.0	2.103157257657779	0.0
78-79	0.0	0.0	0.0	2.250204543671475	0.0
80-81	0.0	0.0	0.0	2.411934142541721	0.0
82-83	0.0	0.0	0.0	2.5727671116192012	0.0
84-85	0.0	0.0	0.0	2.731134348766574	0.0
86-87	0.0	0.0	0.0	2.880199051813994	0.0
88-89	0.0	0.0	0.0	3.045178933683019	0.0
90-91	0.0	0.0	0.0	3.2264102305459357	0.0
92-93	0.0	0.0	0.0	3.4212030530244446	0.0
94-95	0.0	0.0	0.0	3.6182374499848695	0.0
96-97	0.0	0.0	0.0	3.8221086491151386	0.0
98-99	0.0	0.0	0.0	4.022281250350246	0.0
100-101	0.0	0.0	0.0	4.255068760297233	0.0
102-103	0.0	0.0	0.0	4.500184929894758	0.0
104-105	0.0	0.0	0.0	4.75393116124766	0.0
106-107	0.0	0.0	0.0	4.996917835087365	0.0
108-109	0.0	0.0	0.0	5.257052553713729	0.0
110-111	0.0	0.0	0.0	5.537697678849623	0.0
112-113	0.0	0.0	0.0	5.824955448707172	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1935	0.0	49.816677	1
GTATCAA	2940	0.0	37.847305	1
GTCGACG	65	0.001941379	36.600967	9
CCGCAGA	300	0.0	35.701954	1
ATCAACG	3695	0.0	30.744318	3
TATCAAC	3760	0.0	30.371017	2
CAACGCA	3895	0.0	29.623762	5
TCAACGC	3935	0.0	29.322634	4
AACGCAG	4110	0.0	28.0741	6
AGTCGAC	90	0.009541129	26.434032	8
ACGCAGA	4425	0.0	26.075605	7
CGCAGAG	4535	0.0	25.311972	8
GCAGAGT	4810	0.0	23.864822	9
CTCAACG	310	3.3469405E-10	23.033516	1
GAGTACT	2275	0.0	22.744888	12-13
ATGGGAG	425	0.0	22.391182	5
CAGAGTA	4860	0.0	22.08955	10-11
CATGGGT	460	0.0	21.980474	4
GTACTAG	140	0.0031516708	21.251162	1
GTAAGTA	200	4.9153692E-5	20.816803	2
>>END_MODULE