FastQCFastQC Report
Sun 1 Jan 2017
ERR1142725_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142725_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99145
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3390.34192344545867165No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3030.30561299107367995No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2390.2410610721670281No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1920.1936557567199556No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA1890.1906298855212063No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1850.18659539058954056No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG1590.16037117353371325No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1370.13818145140955168No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1370.13818145140955168No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1350.1361642039437188No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC1320.1331383327449695No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC1290.1301124615462202No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT1250.12607796661455445No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA1190.12002622421705582No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1190.12002622421705582No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT1140.11498310555247365No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT1120.1129658580866408No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC1080.10893136315497504No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC1050.10590549195622573No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC1040.10489686822330929No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1040.10489686822330929No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA1040.10489686822330929No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1000.10086237329164356No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGGG150.004240856559.46637796-97
ATGGGGA551.294592E-554.142465
GTACATA551.294592E-554.142461
TACATAA551.294592E-554.142462
GTACAAA751.447559E-647.6453671
ATGGGGC558.450776E-443.313975
GAACAAA700.002763781234.0324061
TACAAAA902.3640048E-433.087062
GCTTTGA750.003875233531.7635781
TGGGCTT1004.385101E-429.7783536
TTAGAAG400.005353156529.74823256-57
CCTGGTG850.007145686528.0266862
GCGGAGG557.731115E-427.04384654-55
AACAAGG600.00127698124.81529414-15
CTATGGA1250.001614034823.8226851
CATGGGC1852.6389647E-522.5349734
ACTATCT802.6106468E-422.29989236-37
CATGGGG4900.021.8779754
TGGACAT1751.5043042E-920.4091130-31
ATGGGCT1500.0046503519.8522365
Produced by FastQC (version 0.11.5)