FastQCFastQC Report
Sun 1 Jan 2017
ERR1142724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283272
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5980.21110452144934905No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5620.19839588805106045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4700.16591826936654522No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3350.11826089412296309No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT750.003882375831.7719021
GTCTAGC800.00532372529.786161
GTAAGTA800.00532372529.786165
GTACAAA1954.701542E-827.4949131
TATACTG900.00946082826.4765855
GCACCGC1159.958463E-425.9010076
ACATAGC1200.001276388124.8217988
GTAAAAA2752.07001E-923.8289241
GAAATAA1400.003127722821.2758271
CATGGGC4850.020.8810184
GTACTGG1450.003833190520.542181
TAACCTC2107.055329E-519.8574374
GTATCAA23600.019.6891561
GGTATCA19750.019.606081
CATGGGT3350.019.5610584
ACAGCGG1908.2329597E-418.812318
ATGTGAG1650.00808780218.0522177
GTATGTG1650.00808780218.0522179
AACGCAG25700.017.6167166
TGTAGCT3053.4862333E-617.5787162
Produced by FastQC (version 0.11.5)