##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142724_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283272
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218853257646362	33.0	33.0	33.0	27.0	33.0
2	31.36992007681663	33.0	33.0	33.0	27.0	33.0
3	31.409800474455647	33.0	33.0	33.0	27.0	33.0
4	31.343073088762743	33.0	33.0	33.0	27.0	33.0
5	31.39487842074049	33.0	33.0	33.0	27.0	33.0
6	34.682856053545706	37.0	37.0	37.0	27.0	37.0
7	34.70061989889576	37.0	37.0	37.0	27.0	37.0
8	34.641531813945605	37.0	37.0	37.0	27.0	37.0
9	34.5638608828264	37.0	37.0	37.0	27.0	37.0
10-11	34.66229983902397	37.0	37.0	37.0	27.0	37.0
12-13	34.6222023355644	37.0	37.0	37.0	27.0	37.0
14-15	34.60887945155187	37.0	37.0	37.0	27.0	37.0
16-17	34.650127792369176	37.0	37.0	37.0	27.0	37.0
18-19	34.61722478748341	37.0	37.0	37.0	27.0	37.0
20-21	34.64665409924031	37.0	37.0	37.0	27.0	37.0
22-23	34.735799867265385	37.0	37.0	37.0	27.0	37.0
24-25	34.80172237284306	37.0	37.0	37.0	27.0	37.0
26-27	34.85375893134514	37.0	37.0	37.0	27.0	37.0
28-29	34.90424397751984	37.0	37.0	37.0	27.0	37.0
30-31	34.94398669829704	37.0	37.0	37.0	27.0	37.0
32-33	34.92941413200034	37.0	37.0	37.0	27.0	37.0
34-35	34.932547163150616	37.0	37.0	37.0	27.0	37.0
36-37	34.938615535598295	37.0	37.0	37.0	27.0	37.0
38-39	34.89070398768675	37.0	37.0	37.0	27.0	37.0
40-41	34.86817793498828	37.0	37.0	37.0	27.0	37.0
42-43	34.85984671976051	37.0	37.0	37.0	27.0	37.0
44-45	34.86972591713971	37.0	37.0	37.0	27.0	37.0
46-47	34.87165868846903	37.0	37.0	37.0	27.0	37.0
48-49	34.84569247931317	37.0	37.0	37.0	27.0	37.0
50-51	34.823410008754834	37.0	37.0	37.0	27.0	37.0
52-53	34.79290399333503	37.0	37.0	37.0	27.0	37.0
54-55	34.77026674009433	37.0	37.0	37.0	27.0	37.0
56-57	34.750917845745434	37.0	37.0	37.0	27.0	37.0
58-59	34.71298787031546	37.0	37.0	37.0	27.0	37.0
60-61	34.68415515829309	37.0	37.0	37.0	27.0	37.0
62-63	34.63345653647378	37.0	37.0	37.0	27.0	37.0
64-65	34.58984121268604	37.0	37.0	37.0	27.0	37.0
66-67	34.54254391538875	37.0	37.0	37.0	27.0	37.0
68-69	34.48907940071733	37.0	37.0	37.0	27.0	37.0
70-71	34.435332471970405	37.0	37.0	37.0	27.0	37.0
72-73	34.389277796605384	37.0	37.0	37.0	27.0	37.0
74-75	34.332291931429864	37.0	37.0	37.0	27.0	37.0
76-77	34.26371826371826	37.0	37.0	37.0	27.0	37.0
78-79	34.1977127990059	37.0	37.0	37.0	27.0	37.0
80-81	34.1507438080714	37.0	37.0	37.0	27.0	37.0
82-83	34.10256926205202	37.0	37.0	37.0	27.0	37.0
84-85	34.05746949927985	37.0	37.0	37.0	27.0	37.0
86-87	33.9748422011353	37.0	37.0	37.0	27.0	37.0
88-89	33.90830721003135	37.0	37.0	37.0	27.0	37.0
90-91	33.83510548165721	37.0	37.0	37.0	22.0	37.0
92-93	33.77826258860742	37.0	37.0	37.0	22.0	37.0
94-95	33.72548822333305	37.0	35.0	37.0	22.0	37.0
96-97	33.63123252562907	37.0	33.0	37.0	22.0	37.0
98-99	33.55216540992403	37.0	33.0	37.0	22.0	37.0
100-101	33.493928097376376	37.0	33.0	37.0	22.0	37.0
102-103	33.42550446214239	37.0	33.0	37.0	22.0	37.0
104-105	33.264989127058094	37.0	33.0	37.0	14.0	37.0
106-107	33.17356639554916	37.0	33.0	37.0	14.0	37.0
108-109	33.07076414188483	37.0	33.0	37.0	14.0	37.0
110-111	32.964945352876384	37.0	33.0	37.0	14.0	37.0
112-113	32.87548010392838	37.0	33.0	37.0	14.0	37.0
114-115	32.75062131096614	37.0	33.0	37.0	14.0	37.0
116-117	32.652344742861985	37.0	33.0	37.0	14.0	37.0
118-119	32.515043844785225	37.0	33.0	37.0	14.0	37.0
120-121	32.36329570165777	37.0	33.0	37.0	14.0	37.0
122-123	32.24063620830862	37.0	33.0	37.0	14.0	37.0
124-125	30.644373605580505	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	527.0
1	20.0
2	16.0
3	15.0
4	11.0
5	16.0
6	5.0
7	4.0
8	4.0
9	8.0
10	6.0
11	11.0
12	7.0
13	9.0
14	28.0
15	121.0
16	185.0
17	333.0
18	704.0
19	1411.0
20	2129.0
21	2491.0
22	2584.0
23	2623.0
24	2784.0
25	2969.0
26	3560.0
27	4199.0
28	5112.0
29	6226.0
30	7378.0
31	9153.0
32	11575.0
33	15693.0
34	23579.0
35	44873.0
36	132903.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.611144554848124	19.920820968987034	12.267438154661152	24.200596321503692
2	17.132297758457117	22.98431061582249	35.95389473386103	23.929496891859362
3	19.68693472851039	27.721356332987607	27.929500136284634	24.66220880221737
4	14.54582789006546	19.556466602232536	33.62364095320029	32.27406455450171
5	15.334036767413533	37.83672775684786	30.92407160864516	15.905163867093448
6	29.36998026649094	36.328079330832104	18.122234385905244	16.17970601677171
7	26.081318556730253	33.529024120631505	21.453827839346957	18.93582948329129
8	25.35253684807256	34.39165249433106	20.21967120181406	20.036139455782312
9	26.002387945481146	18.921810728748525	19.79266681074646	35.28313451502386
10-11	24.850614989698503	27.727594019993585	25.299522924597994	22.122268065709918
12-13	25.683245989816417	26.642961671609637	24.808393481657863	22.865398856916084
14-15	24.25946298333537	26.90291573440758	23.078749098742403	25.758872183514647
16-17	23.888444353176936	28.54090397320512	24.04475963206605	23.525892041551895
18-19	23.93659672009275	27.634767370852547	25.574426281739253	22.85420962731545
20-21	25.618818232817585	26.330058806977558	24.962615185048197	23.088507775156664
22-23	25.465613193774715	26.19325270077772	24.645072241933057	23.696061863514505
24-25	24.419232177961167	26.97887489671012	24.751706163066192	23.85018676226252
26-27	24.218159682468336	26.815180788131137	24.927757299919133	24.038902229481394
28-29	24.03302942534674	26.70167535691348	25.33665491256969	23.92864030517009
30-31	24.54213296766488	26.5643384792321	25.26792586367054	23.625602689432476
32-33	23.86991179410958	27.068680273553277	24.946607567835372	24.11480036450177
34-35	24.119572525567076	26.834990622456562	25.18082734703988	23.864609504936478
36-37	24.133202881037768	26.99424818608206	24.742707616420713	24.12984131645946
38-39	24.082658900216927	26.950842761241827	24.907372347239573	24.059125991301674
40-41	23.836010006121143	26.98061402484547	25.16992360956313	24.013452359470257
42-43	24.00954670827871	26.984843096770998	24.990401984356563	24.015208210593734
44-45	23.98460926097917	27.088408296846666	24.90168502056521	24.02529742160895
46-47	24.01073473113638	26.858410523857714	25.093230113857494	24.03762463114841
48-49	24.05862330070979	27.06727809269049	25.100312082032993	23.773786524566727
50-51	23.62723103706954	27.516418734342047	24.894375168079716	23.961975060508696
52-53	23.609570366753186	27.230570255288804	24.85036349771675	24.309495880241258
54-55	23.580489358314313	27.06225784194045	24.94559736744334	24.411655432301895
56-57	23.98351833046137	26.708938245045417	24.777525768088967	24.53001765640425
58-59	23.823028827611097	26.841488830937227	25.105887598147277	24.2295947433044
60-61	23.83091584009512	26.912379287526583	25.0959861001511	24.160718772227195
62-63	23.47406377279306	27.008602356005195	25.056883123316947	24.460450747884796
64-65	23.499114446117453	26.671538245134762	25.225811816703615	24.60353549204417
66-67	24.001656385045816	26.46340557012561	25.054593460110496	24.48034458471808
68-69	23.99745182353878	26.44157774592557	24.91780361345579	24.643166817079862
70-71	23.83203037906591	26.32758004409636	25.190488492831687	24.649901084006046
72-73	24.144130752542036	26.54831551341537	25.16961659747513	24.137937136567462
74-75	24.232074059260857	26.237344286723026	25.241345624631677	24.28923602938444
76-77	24.00552799031717	26.277949618136905	25.367176053397127	24.349346338148795
78-79	23.903303097118062	26.35222418448928	25.48990808804012	24.254564630352533
80-81	23.796993944713215	26.361908671694458	25.459455059579632	24.3816423240127
82-83	24.21738568868113	26.15585229011888	25.50332720558963	24.123434815610352
84-85	23.931965664385647	26.49209898733998	25.132155316995846	24.44378003127853
86-87	23.646861738165956	26.434953661526595	25.417435436593504	24.50074916371394
88-89	24.016103897022795	25.89500980844782	25.483037311017124	24.605848983512253
90-91	24.199465779837613	26.015991226052964	25.36077550370593	24.423767490403495
92-93	23.80659255131913	26.427870200854358	25.432531154094473	24.333006093732035
94-95	23.301867269456693	26.56185053708096	25.537213617253094	24.599068576209252
96-97	23.473558181197596	26.532720015421376	25.364798593683958	24.628923209697074
98-99	23.415051724717323	26.904104549546602	25.12712306573965	24.55372065999643
100-101	23.66981434087207	27.009803834868258	24.90683438540762	24.413547438852053
102-103	23.570070481698636	27.088495653481193	25.15321147162603	24.18822239319414
104-105	23.503836588567	27.266488132930984	25.097197114687287	24.132478163814724
106-107	23.461098327340387	27.148307265642824	25.260573730913517	24.13002067610327
108-109	23.489447357063515	27.238943249916865	25.08578665478035	24.185822738239267
110-111	23.34207044874108	27.761034587657672	24.840644919385078	24.05625004421617
112-113	23.290520440179268	27.778092202771106	24.66333926417477	24.268048092874857
114-115	23.192509519623776	27.765643045501413	24.853778932493352	24.188068502381455
116-117	23.423574792760636	27.722409795387055	24.71856655978239	24.135448852069917
118-119	23.2180724532333	27.411113646131234	25.07140797965381	24.299405920981656
120-121	23.202722469749844	27.48983407296827	25.29834497469794	24.00909848258394
122-123	23.205325702279236	27.611162892702453	25.165853391085037	24.01765801393328
124-125	23.189174941312373	27.67278574726552	24.953253337720533	24.184785973701576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	526.0
2	145.5
3	103.0
4	88.5
5	74.0
6	64.5
7	56.5
8	57.5
9	69.0
10	58.0
11	48.5
12	70.0
13	76.5
14	67.0
15	76.0
16	94.0
17	114.5
18	131.0
19	148.0
20	190.0
21	241.0
22	307.0
23	358.5
24	452.5
25	587.5
26	730.5
27	887.0
28	1099.0
29	1496.5
30	1973.5
31	2437.5
32	2968.5
33	3467.5
34	3939.0
35	4424.5
36	4970.5
37	5758.0
38	6233.5
39	6311.0
40	6446.5
41	6590.5
42	6911.5
43	7556.0
44	8379.5
45	9203.5
46	10138.0
47	11145.5
48	12065.5
49	12714.5
50	13291.5
51	13024.0
52	12054.5
53	11764.5
54	11516.0
55	10987.0
56	10300.5
57	9209.5
58	8347.0
59	7518.0
60	6374.0
61	5413.0
62	4540.0
63	3543.5
64	2582.5
65	1988.5
66	1615.0
67	1230.0
68	949.0
69	781.0
70	678.5
71	605.5
72	524.0
73	478.5
74	396.0
75	333.0
76	269.5
77	170.5
78	109.5
79	73.0
80	46.5
81	31.0
82	19.5
83	11.5
84	4.0
85	1.0
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3085373775028947
2	0.27817786438476094
3	0.27358863565760116
4	0.28700330424468357
5	0.2993589200485752
6	0.3569007879352707
7	0.36149001666243047
8	0.3643141574176057
9	0.36043096387923973
10-11	0.3641376486204072
12-13	0.3717275268999407
14-15	0.36272557824281965
16-17	0.2930046033494309
18-19	0.2804724787483408
20-21	0.2603504758677172
22-23	0.25223107119658844
24-25	0.24481770171425346
26-27	0.2532901239797792
28-29	0.23846338501510916
30-31	0.24164054336468127
32-33	0.24517071930865034
34-35	0.24075799937868905
36-37	0.2349332090711401
38-39	0.24322912253946735
40-41	0.22769634838600358
42-43	0.23352113869355245
44-45	0.22398966364483605
46-47	0.22522522522522523
48-49	0.2308735067355757
50-51	0.23581575305713234
52-53	0.23669829704312464
54-55	0.2319325595187664
56-57	0.23122652432997262
58-59	0.23334462989635404
60-61	0.23969894659549834
62-63	0.2382868762179107
64-65	0.24022847298709368
66-67	0.2573498263153436
68-69	0.2531136151825807
70-71	0.25028947442740546
72-73	0.2552317207489621
74-75	0.2617625462453049
76-77	0.25064249202180233
78-79	0.2538196503713745
80-81	0.25028947442740546
82-83	0.23916942020390297
84-85	0.2294614363579881
86-87	0.2201064701064701
88-89	0.21445818859611965
90-91	0.21781185574289022
92-93	0.21339913581292894
94-95	0.20704481911378464
96-97	0.19327713293230533
98-99	0.20245559038662486
100-101	0.20351464316981557
102-103	0.20280860798102177
104-105	0.21145753904374592
106-107	0.2042206783586094
108-109	0.2096924510717614
110-111	0.20122002880623568
112-113	0.2008670112118388
114-115	0.19998446722584654
116-117	0.20033748482024347
118-119	0.19963144963144963
120-121	0.20739783670818152
122-123	0.20033748482024347
124-125	0.22293061086164534
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	283272.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.66756343775864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.63410657589917	66.3105931864315
2	8.400952299677746	12.713591821469002
3	1.870019361021489	4.244994258897913
4	0.6667312116256541	2.0179970500647144
5	0.3639720980867825	1.3770440910777861
6	0.2013292327484178	0.9140455494519707
7	0.13368969583850954	0.7081181478584555
8	0.10267370509561805	0.6215255274969921
9	0.0747370976387381	0.508965666905986
>10	0.5099472301372621	7.305497194989212
>50	0.031125988122771904	1.5807052664541437
>100	0.00969497990709288	1.249042876643662
>500	0.0010205242007466188	0.4478793622586238
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	598	0.21110452144934905	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	562	0.19839588805106045	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	470	0.16591826936654522	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	335	0.11826089412296309	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.5301759439690475E-4	0.0
22-23	0.0	0.0	0.0	0.0024711231607783333	3.5301759439690475E-4
24-25	0.0	0.0	0.0	0.00670733429354119	3.5301759439690475E-4
26-27	0.0	0.0	0.0	0.01482673896467	3.5301759439690475E-4
28-29	0.0	0.0	0.0	0.02982998672653845	3.5301759439690475E-4
30-31	0.0	0.0	0.0	0.0522466039707419	3.5301759439690475E-4
32-33	0.0	0.0	0.0	0.09037250416560762	3.5301759439690475E-4
34-35	0.0	0.0	0.0	0.13926544098957894	3.5301759439690475E-4
36-37	0.0	0.0	0.0	0.19574825609308366	3.5301759439690475E-4
38-39	0.0	0.0	0.0	0.2801194611539439	3.5301759439690475E-4
40-41	0.0	0.0	0.0	0.37384563246632213	3.5301759439690475E-4
42-43	0.0	3.5301759439690475E-4	0.0	0.4553926967720071	3.5301759439690475E-4
44-45	0.0	3.5301759439690475E-4	0.0	0.541175972210455	3.5301759439690475E-4
46-47	0.0	3.5301759439690475E-4	0.0	0.6414329690191759	3.5301759439690475E-4
48-49	0.0	3.5301759439690475E-4	0.0	0.735512157925951	3.5301759439690475E-4
50-51	0.0	3.5301759439690475E-4	0.0	0.822884012539185	3.5301759439690475E-4
52-53	0.0	3.5301759439690475E-4	0.0	0.9120209551244034	3.5301759439690475E-4
54-55	0.0	3.5301759439690475E-4	0.0	0.9918029314581038	3.5301759439690475E-4
56-57	0.0	3.5301759439690475E-4	0.0	1.086588155553673	3.5301759439690475E-4
58-59	0.0	3.5301759439690475E-4	0.0	1.2002598209494761	3.5301759439690475E-4
60-61	0.0	3.5301759439690475E-4	0.0	1.3077536784433337	3.5301759439690475E-4
62-63	0.0	3.5301759439690475E-4	0.0	1.4134824479652066	3.5301759439690475E-4
64-65	0.0	3.5301759439690475E-4	0.0	1.5089737072495693	3.5301759439690475E-4
66-67	0.0	3.5301759439690475E-4	0.0	1.6244104606173573	3.5301759439690475E-4
68-69	0.0	3.5301759439690475E-4	0.0	1.7467310570758847	3.5301759439690475E-4
70-71	0.0	3.5301759439690475E-4	0.0	1.8879380948346465	3.5301759439690475E-4
72-73	0.0	3.5301759439690475E-4	0.0	2.0127298144539525	3.5301759439690475E-4
74-75	0.0	3.5301759439690475E-4	0.0	2.137521534073258	3.5301759439690475E-4
76-77	0.0	3.5301759439690475E-4	0.0	2.2660199384337316	3.5301759439690475E-4
78-79	0.0	3.5301759439690475E-4	0.0	2.4195825919963854	3.5301759439690475E-4
80-81	0.0	3.5301759439690475E-4	0.0	2.579676071055381	3.5301759439690475E-4
82-83	0.0	3.5301759439690475E-4	0.0	2.7526546923098647	3.5301759439690475E-4
84-85	0.0	3.5301759439690475E-4	0.0	2.909217995424892	3.5301759439690475E-4
86-87	0.0	3.5301759439690475E-4	0.0	3.065251772148324	5.295263915953572E-4
88-89	0.0	3.5301759439690475E-4	0.0	3.239642463780395	7.060351887938095E-4
90-91	0.0	3.5301759439690475E-4	0.0	3.414915699398458	7.060351887938095E-4
92-93	0.0	3.5301759439690475E-4	0.0	3.601838515631619	7.060351887938095E-4
94-95	0.0	3.5301759439690475E-4	0.0	3.7933505605919398	7.060351887938095E-4
96-97	0.0	3.5301759439690475E-4	0.0	3.982744499985879	7.060351887938095E-4
98-99	0.0	3.5301759439690475E-4	0.0	4.187141687141687	7.060351887938095E-4
100-101	0.0	3.5301759439690475E-4	0.0	4.416779632296874	7.060351887938095E-4
102-103	0.0	3.5301759439690475E-4	0.0	4.656302070095173	7.060351887938095E-4
104-105	0.0	3.5301759439690475E-4	0.0	4.905709000536587	7.060351887938095E-4
106-107	0.0	3.5301759439690475E-4	0.0	5.143289841565704	7.060351887938095E-4
108-109	0.0	3.5301759439690475E-4	0.0	5.395520912762292	7.060351887938095E-4
110-111	0.0	3.5301759439690475E-4	0.0	5.682700725804175	7.060351887938095E-4
112-113	0.0	3.5301759439690475E-4	0.0	5.980647575475162	7.060351887938095E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTACT	75	0.0038823758	31.771902	1
GTCTAGC	80	0.005323725	29.78616	1
GTAAGTA	80	0.005323725	29.78616	5
GTACAAA	195	4.701542E-8	27.494913	1
TATACTG	90	0.009460828	26.476585	5
GCACCGC	115	9.958463E-4	25.901007	6
ACATAGC	120	0.0012763881	24.821798	8
GTAAAAA	275	2.07001E-9	23.828924	1
GAAATAA	140	0.0031277228	21.275827	1
CATGGGC	485	0.0	20.881018	4
GTACTGG	145	0.0038331905	20.54218	1
TAACCTC	210	7.055329E-5	19.857437	4
GTATCAA	2360	0.0	19.689156	1
GGTATCA	1975	0.0	19.60608	1
CATGGGT	335	0.0	19.561058	4
ACAGCGG	190	8.2329597E-4	18.81231	8
ATGTGAG	165	0.008087802	18.052217	7
GTATGTG	165	0.008087802	18.052217	9
AACGCAG	2570	0.0	17.616716	6
TGTAGCT	305	3.4862333E-6	17.578716	2
>>END_MODULE