FastQCFastQC Report
Sun 1 Jan 2017
ERR1142724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283272
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.5351746731057077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13960.49281256177807903No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.27888389957355475No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5180.18286311389759666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.1493264424298907No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4070.14367816091954022No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2940.10378717275268999No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.10237510237510238No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACG1858.076313E-1032.1460381
GAATAAC750.003915156231.7174221
GGTATCA18550.030.7770141
GTATAGG800.005368612229.7350833
AAGCTAG800.005368612229.7350835
GTGTTAT1107.759442E-427.0318951
TAACGCC1150.00100587925.8565944
GTATCAA23050.025.0264912
GATAGCA1200.001289230824.7792381
GAGTACT13650.024.1801812-13
AGTGAAC1300.002054348122.8731423
TGTTATC1300.002054348122.8731422
CAACGCA27950.021.7028886
ATGGGTA1653.2179017E-421.6255135
ATCAACG26900.021.4446334
GTATTAA1400.003159039421.2393461
AACGCAG28950.021.1586427
TATCAAC27300.021.1304263
TCAACGC28850.020.8197145
CCGCAGA2607.689996E-720.5858291
Produced by FastQC (version 0.11.5)