ERR1142723_2.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1142723_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119555
Sequences flagged as poor quality	0
Sequence length	125
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	271	0.22667391577098406	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	238	0.19907155702396387	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	229	0.19154364100204926	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	222	0.1856885952072268	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	221	0.18485216009368075	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	220	0.1840157249801347	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	212	0.17732424407176614	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	208	0.17397850361758185	No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT	207	0.1731420685040358	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	203	0.16979632804985154	No Hit
GTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAA	195	0.16310484714148302	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	187	0.15641336623311447	No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	181	0.15139475555183807	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	172	0.14386683952992346	No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGA	172	0.14386683952992346	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	171	0.1430304044163774	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	167	0.13968466396219315	No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTG	154	0.12881100748609425	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	150	0.12546526703191	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	149	0.12462883191836392	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	149	0.12462883191836392	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	148	0.12379239680481786	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	147	0.1229559616912718	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	142	0.11877378612354147	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	137	0.11459161055581114	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	135	0.11291874032871899	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	129	0.1079001296474426	No Hit
TATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAA	128	0.10706369453389654	No Hit
GGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTG	128	0.10706369453389654	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	127	0.10622725942035047	No Hit
ACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCT	126	0.10539082430680441	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	126	0.10539082430680441	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	124	0.10371795407971227	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	121	0.10120864873907408	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	120	0.10037221362552799	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATC	30	3.5215453E-7	99.34033	2
CTGCGTA	40	1.9456165E-6	74.53657	9
TCGCAAT	40	1.9553663E-6	74.47396	1
ATCCTGC	45	3.9161787E-6	66.25473	6
TCCTGCG	45	3.9161787E-6	66.25473	7
GGATTAC	15	0.0042382935	59.479244	80-81
TGCAACG	15	0.004245337	59.454315	100-101
TTCTCTG	65	5.358688E-7	55.042393	7
GTACAAA	65	5.38992E-7	54.99616	1
CGCAACT	35	9.105988E-7	51.003593	24-25
GCTTTGA	50	5.256684E-4	47.703403	8
TATTCTC	75	1.4421475E-6	47.68336	5
CGTAATC	40	2.2555578E-6	44.712543	12-13
GTAAAAA	95	1.4572288E-7	43.90044	1
GTACAGA	55	8.4421213E-4	43.330303	1
AATCCTG	70	5.3667634E-5	42.57443	5
CAATCCT	70	5.3667634E-5	42.57443	4
AACGCCG	35	5.4366683E-5	42.502995	18-19
CCGCAAC	35	5.4366683E-5	42.502995	22-23
TCTCTGC	90	5.0408744E-6	39.75284	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content