FastQCFastQC Report
Sun 1 Jan 2017
ERR1142713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1142713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659838
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15320.23217820131608063No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12460.18883422900772615No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28650.030.5803931
AACGCAG33850.025.358316
ATCAACG33650.025.1509063
GGTATCA21150.025.0801431
TCAACGC34150.024.7826654
CAACGCA34150.024.6081395
TATCAAC35300.024.478092
ACGCAGA37150.023.1057557
ATGGGGG12900.023.1009925
CGCAGAG37450.022.9206648
GGGTATA1602.5799655E-422.3502121
CATGGGG25500.022.204134
TCTATAC1903.2600852E-521.9581013
GTACATG51800.021.861211
CATGGGT11300.021.6249834
ACATGGG52350.021.2899823
TACATGG53000.021.250552
GCAGAGT41400.020.7337889
GTATTGA2357.2793628E-620.2895531
ATGGGTA6050.019.7026655
Produced by FastQC (version 0.11.5)