##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1142713_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	659838
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202736126140053	33.0	33.0	33.0	27.0	33.0
2	31.34426480439138	33.0	33.0	33.0	27.0	33.0
3	31.392534228098413	33.0	33.0	33.0	27.0	33.0
4	31.369546767539912	33.0	33.0	33.0	27.0	33.0
5	31.38357142207633	33.0	33.0	33.0	27.0	33.0
6	34.68439374513138	37.0	37.0	37.0	27.0	37.0
7	34.6843755588493	37.0	37.0	37.0	27.0	37.0
8	34.66316732288835	37.0	37.0	37.0	27.0	37.0
9	34.600239755818855	37.0	37.0	37.0	27.0	37.0
10-11	34.66784195514657	37.0	37.0	37.0	27.0	37.0
12-13	34.637122293653896	37.0	37.0	37.0	27.0	37.0
14-15	34.62476092616673	37.0	37.0	37.0	27.0	37.0
16-17	34.66962193750587	37.0	37.0	37.0	27.0	37.0
18-19	34.62427595864439	37.0	37.0	37.0	27.0	37.0
20-21	34.640945959462776	37.0	37.0	37.0	27.0	37.0
22-23	34.71313867949406	37.0	37.0	37.0	27.0	37.0
24-25	34.74333321209146	37.0	37.0	37.0	27.0	37.0
26-27	34.79459276367836	37.0	37.0	37.0	27.0	37.0
28-29	34.810612604912116	37.0	37.0	37.0	27.0	37.0
30-31	34.81945871562414	37.0	37.0	37.0	27.0	37.0
32-33	34.81957162212544	37.0	37.0	37.0	27.0	37.0
34-35	34.82504872408076	37.0	37.0	37.0	27.0	37.0
36-37	34.805578035820915	37.0	37.0	37.0	27.0	37.0
38-39	34.780740878821774	37.0	37.0	37.0	27.0	37.0
40-41	34.753048475534904	37.0	37.0	37.0	27.0	37.0
42-43	34.73238734356009	37.0	37.0	37.0	27.0	37.0
44-45	34.747700950839445	37.0	37.0	37.0	27.0	37.0
46-47	34.734265834947365	37.0	37.0	37.0	27.0	37.0
48-49	34.707239504241954	37.0	37.0	37.0	27.0	37.0
50-51	34.6996997747932	37.0	37.0	37.0	27.0	37.0
52-53	34.65853588305008	37.0	37.0	37.0	27.0	37.0
54-55	34.625348949287556	37.0	37.0	37.0	27.0	37.0
56-57	34.59273791445779	37.0	37.0	37.0	27.0	37.0
58-59	34.551313352671414	37.0	37.0	37.0	27.0	37.0
60-61	34.53195178210409	37.0	37.0	37.0	27.0	37.0
62-63	34.49215034599402	37.0	37.0	37.0	27.0	37.0
64-65	34.45715615044905	37.0	37.0	37.0	27.0	37.0
66-67	34.41310973299507	37.0	37.0	37.0	27.0	37.0
68-69	34.37005598343836	37.0	37.0	37.0	27.0	37.0
70-71	34.33475489438317	37.0	37.0	37.0	27.0	37.0
72-73	34.27421124579064	37.0	37.0	37.0	27.0	37.0
74-75	34.22728306038755	37.0	37.0	37.0	27.0	37.0
76-77	34.176129595446156	37.0	37.0	37.0	27.0	37.0
78-79	34.12263237340075	37.0	37.0	37.0	27.0	37.0
80-81	34.0629700017277	37.0	37.0	37.0	27.0	37.0
82-83	34.027512055989504	37.0	37.0	37.0	27.0	37.0
84-85	33.97947374961733	37.0	37.0	37.0	27.0	37.0
86-87	33.89743012678869	37.0	37.0	37.0	27.0	37.0
88-89	33.82815403174719	37.0	37.0	37.0	24.5	37.0
90-91	33.75320912102668	37.0	33.0	37.0	22.0	37.0
92-93	33.70020595964464	37.0	33.0	37.0	22.0	37.0
94-95	33.650845359012365	37.0	33.0	37.0	22.0	37.0
96-97	33.53169944744013	37.0	33.0	37.0	22.0	37.0
98-99	33.45045298997633	37.0	33.0	37.0	22.0	37.0
100-101	33.36856470830719	37.0	33.0	37.0	22.0	37.0
102-103	33.28629224142895	37.0	33.0	37.0	18.0	37.0
104-105	33.12145935820611	37.0	33.0	37.0	14.0	37.0
106-107	33.017211042710485	37.0	33.0	37.0	14.0	37.0
108-109	32.92025542633192	37.0	33.0	37.0	14.0	37.0
110-111	32.816867170426676	37.0	33.0	37.0	14.0	37.0
112-113	32.70850041980002	37.0	33.0	37.0	14.0	37.0
114-115	32.593594943001165	37.0	33.0	37.0	14.0	37.0
116-117	32.50519142577421	37.0	33.0	37.0	14.0	37.0
118-119	32.372452026103375	37.0	33.0	37.0	14.0	37.0
120-121	32.244754015379534	37.0	33.0	37.0	14.0	37.0
122-123	32.10753700150643	37.0	33.0	37.0	14.0	37.0
124-125	30.482092574237917	35.0	27.5	37.0	14.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	1165.0
1	70.0
2	29.0
3	25.0
4	32.0
5	13.0
6	16.0
7	14.0
8	16.0
9	13.0
10	17.0
11	26.0
12	22.0
13	26.0
14	77.0
15	261.0
16	395.0
17	651.0
18	1384.0
19	2645.0
20	4085.0
21	5142.0
22	5664.0
23	6281.0
24	7146.0
25	8404.0
26	9343.0
27	11623.0
28	13426.0
29	16314.0
30	18773.0
31	22748.0
32	28643.0
33	38289.0
34	56904.0
35	104286.0
36	295870.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.989760101182846	19.352283834915077	12.252306485522613	24.405649578379464
2	17.492815491487253	22.494236436058255	35.968438313528765	24.044509758925734
3	19.896817919338663	26.849375436890256	28.22417408746923	25.029632556301856
4	14.756455265277587	18.681894861624038	34.017690238453476	32.54395963464491
5	15.568104090349449	37.22689203362265	31.195944610801195	16.00905926522671
6	30.330159985400574	35.502007421376	18.228298558306467	15.939534034916965
7	26.686072507277192	32.67283516897985	21.612114354542882	19.028977969200074
8	25.674154739996048	33.71534167820043	20.632557149158163	19.977946432645364
9	26.596534239366832	17.852895281954257	19.856186446606785	35.69438403207212
10-11	25.355568990231976	26.75442298698484	25.869863612685883	22.020144410097302
12-13	25.91417435117407	25.529118737522577	25.549729061697878	23.006977849605477
14-15	24.485157180955625	26.048183554078935	23.470338316047066	25.996320948918378
16-17	24.16418744086904	27.93519436750295	24.152788701569605	23.7478294900584
18-19	24.252631546953726	27.072355098700584	25.496048405418936	23.17896494892675
20-21	25.896834754492907	25.911952934598297	24.801944243544007	23.38926806736479
22-23	25.559619716116487	25.762979675398917	24.834603858293736	23.842796750190864
24-25	24.70854715656874	26.30314437891756	24.769769724149036	24.21853874036466
26-27	24.392372782858587	26.35098538352207	24.973279215673763	24.28336261794558
28-29	24.33425969739112	26.420135848162374	25.15591094448879	24.08969350995772
30-31	24.70256360399336	26.09822085032174	25.281106256797898	23.918109288887
32-33	24.240470032396384	26.238639427879118	25.051784839404334	24.469105700320164
34-35	24.524608840953974	26.419185781558557	25.031292723682213	24.024912653805256
36-37	24.58008738891256	26.51508419518427	24.690671655110762	24.21415676079241
38-39	24.436567084823313	26.35837614653813	24.841406318834064	24.363650449804492
40-41	24.27039879828157	26.466891609159006	25.088985943752224	24.173723648807204
42-43	24.507025138604085	26.377306903622692	24.990582516898307	24.125085440874912
44-45	24.30362328525777	26.39115867364917	24.963700319558694	24.341517721534366
46-47	24.16869491917999	26.21917277673781	25.120764875855755	24.49136742822644
48-49	24.363561247048583	26.457594925653567	24.99428513276339	24.18455869453446
50-51	24.04158055948592	26.72789675188904	24.98281653835083	24.247706150274215
52-53	24.105419772017324	26.377803549688267	25.098144650290617	24.41863202800379
54-55	23.984680207301736	26.19561519159614	25.29975818847932	24.519946412622804
56-57	24.237356742146876	26.065619710063043	24.86183985194062	24.835183695849462
58-59	24.186301552968626	26.245230514982865	25.084757090431864	24.48371084161664
60-61	24.160450949313013	26.243554856158134	25.276583916171546	24.319410278357303
62-63	23.76882964196149	26.368637946826624	25.178973686728835	24.683558724483053
64-65	23.980039798878945	25.937704121158724	25.29894730446142	24.783308775500902
66-67	24.1796824851813	25.929743826206252	25.15582139620529	24.73475229240716
68-69	24.187740988657392	25.815449404915636	25.119487835485078	24.877321770941897
70-71	23.918852812669012	25.854556724335954	25.365750278015785	24.860840184979246
72-73	24.124144731194722	26.084796478879124	25.199502582423715	24.591556207502435
74-75	24.097995511818322	25.886658978718362	25.389145407058635	24.626200102404685
76-77	24.02989246854424	25.67575574346805	25.493523501046756	24.80082828694096
78-79	24.098468494062207	25.72279757717918	25.42199589512048	24.756738033638133
80-81	24.009765427681828	25.7784198936407	25.519244106067617	24.69257057260986
82-83	24.026265019975998	25.85691716416777	25.564948578893798	24.551869236962435
84-85	23.886968679962056	26.023223262451424	25.228963079584748	24.860844978001772
86-87	23.719954711376147	25.990157090167948	25.49679661200999	24.793091586445914
88-89	23.838931474499887	25.60049508145685	25.49267059238926	25.067902851654004
90-91	24.226681996084622	25.58901567215296	25.40084048545857	24.783461846303855
92-93	24.03884704167173	26.028580974365205	25.232960758109584	24.69961122585348
94-95	23.54556187858518	26.088583999908888	25.43105640994491	24.93479771156102
96-97	23.689608575506362	25.96823661595457	25.328034374905105	25.014120433633963
98-99	23.43809197694009	26.05970278439379	25.359371524063256	25.142833714602865
100-101	23.678416270972306	26.322874449355933	25.172235239094555	24.82647404057721
102-103	23.846082595234154	26.18276818700166	25.396204755519992	24.574944462244197
104-105	23.67532458657401	26.286216298919573	25.353782091340587	24.684677023165825
106-107	23.64841779504148	26.271481133350445	25.507597833398883	24.572503238209197
108-109	23.696349589020294	26.245372918999983	25.330169517264945	24.72810797471478
110-111	23.42597745534003	26.630547429655177	25.21343933236101	24.730035782643785
112-113	23.565938909054466	26.633240987555716	25.23317372116029	24.567646382229526
114-115	23.486435372424626	26.67949244536833	25.182156173342374	24.651916008864667
116-117	23.439666181264908	26.781877457061686	25.264174465235566	24.51428189643784
118-119	23.206888802507226	26.381617307645588	25.48415065231651	24.92734323753067
120-121	23.299335868780364	26.419165732135006	25.631729482126463	24.649768916958163
122-123	23.31594455533344	26.615839099012778	25.502815585062404	24.565400760591373
124-125	23.308280954192647	26.58210064844579	25.245113115402695	24.86450528195887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2003.0
1	1227.0
2	355.5
3	236.0
4	180.0
5	141.5
6	138.0
7	120.0
8	119.0
9	124.0
10	123.0
11	125.5
12	123.0
13	133.5
14	137.5
15	151.5
16	173.5
17	202.5
18	228.5
19	286.0
20	364.5
21	420.0
22	527.5
23	684.0
24	894.0
25	1173.0
26	1514.0
27	2001.5
28	2566.0
29	3504.5
30	4651.0
31	5631.5
32	6741.5
33	7792.5
34	9001.5
35	10164.0
36	10995.0
37	12175.5
38	13297.0
39	14044.0
40	14546.5
41	15141.5
42	16032.0
43	17415.5
44	19334.5
45	21002.5
46	22699.0
47	24411.5
48	26288.5
49	27901.5
50	28577.0
51	28236.5
52	27668.5
53	27664.0
54	27089.5
55	26442.5
56	25663.0
57	23625.0
58	21581.5
59	19306.0
60	16494.0
61	13817.0
62	11294.0
63	8780.0
64	6718.0
65	5473.0
66	4305.0
67	3267.5
68	2660.0
69	2233.5
70	1853.0
71	1632.5
72	1494.0
73	1396.5
74	1100.0
75	886.5
76	704.5
77	496.5
78	385.5
79	248.5
80	171.5
81	124.0
82	78.0
83	45.5
84	31.5
85	24.0
86	12.5
87	5.0
88	3.0
89	1.5
90	1.0
91	1.0
92	1.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.305984196120866
2	0.2765830400795347
3	0.2694600795952946
4	0.28006874414629046
5	0.2952239792191417
6	0.3452362549595507
7	0.349176616078492
8	0.35584491951054653
9	0.3516014536901482
10-11	0.3539505151264401
12-13	0.3638014179237934
14-15	0.35334430572352604
16-17	0.28347867203768196
18-19	0.27468863569542823
20-21	0.2561234727311855
22-23	0.24937939312376675
24-25	0.24005892355396324
26-27	0.24892473607158122
28-29	0.23179932043925935
30-31	0.23429993422627976
32-33	0.24028625208005602
34-35	0.2330875154204517
36-37	0.22982913987978867
38-39	0.23520924833065085
40-41	0.21937202767952133
42-43	0.22550989788402606
44-45	0.21671986154177236
46-47	0.2167956377171366
48-49	0.2224030746940916
50-51	0.227555854618861
52-53	0.22710119756667546
54-55	0.22338816497382688
56-57	0.22028134178389241
58-59	0.22520679318256903
60-61	0.2263434358130329
62-63	0.22748007844349674
64-65	0.23157199191316657
66-67	0.24740921256429607
68-69	0.24430238937436158
70-71	0.24278686586707648
72-73	0.24831852666866716
74-75	0.2523346639629727
76-77	0.24430238937436158
78-79	0.24202910411343392
80-81	0.24210488028879817
82-83	0.2346030389277368
84-85	0.22573722641011884
86-87	0.2113397530909102
88-89	0.20618697316614076
90-91	0.2135372621764736
92-93	0.20580809228931948
94-95	0.19860935562971516
96-97	0.1845907631873278
98-99	0.19588141331660197
100-101	0.19565408479050916
102-103	0.19353235188031
104-105	0.20406524025594158
106-107	0.19626029419342322
108-109	0.20467144965885564
110-111	0.1937596804064028
112-113	0.19133484279474658
114-115	0.19224415689911767
116-117	0.19353235188031
118-119	0.19125906661938233
120-121	0.19830625092825815
122-123	0.1926988139513032
124-125	0.214825457157666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	659838.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.00510142801082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02356021651092	71.30357423393085
2	8.532405271464334	13.823367088797253
3	1.7892287544876377	4.348099702055537
4	0.6594887080044229	2.1368779873010437
5	0.31891437979594905	1.291684584111101
6	0.19557623161691795	0.9505603487421944
7	0.11271593870120677	0.6391396232931293
8	0.0786006460766179	0.509364264619489
9	0.0599442720580185	0.4370212654279257
>10	0.21405120202472291	2.8093100922805885
>50	0.010416809197707008	0.5743321812719225
>100	0.004654303099664938	0.6779219926788967
>500	0.0	0.0
>1k	4.432669618514921E-4	0.4987466354900372
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1532	0.23217820131608063	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	1246	0.18883422900772615	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.5155235072851215E-4	0.0
6	0.0	0.0	0.0	1.5155235072851215E-4	0.0
7	0.0	0.0	0.0	1.5155235072851215E-4	0.0
8	0.0	0.0	0.0	1.5155235072851215E-4	0.0
9	0.0	0.0	0.0	1.5155235072851215E-4	0.0
10-11	0.0	0.0	0.0	1.5155235072851215E-4	0.0
12-13	0.0	0.0	0.0	3.031047014570243E-4	0.0
14-15	0.0	0.0	0.0	3.031047014570243E-4	0.0
16-17	0.0	0.0	0.0	3.031047014570243E-4	0.0
18-19	0.0	0.0	0.0	3.031047014570243E-4	1.5155235072851215E-4
20-21	0.0	0.0	0.0	6.062094029140486E-4	1.5155235072851215E-4
22-23	0.0	0.0	0.0	0.0017428520333778899	1.5155235072851215E-4
24-25	0.0	0.0	0.0	0.0044707943464911085	1.5155235072851215E-4
26-27	0.0	0.0	0.0	0.008411155465432425	1.5155235072851215E-4
28-29	0.0	0.0	0.0	0.018262058262785714	1.5155235072851215E-4
30-31	0.0	0.0	0.0	0.03728187827921399	1.5155235072851215E-4
32-33	0.0	0.0	0.0	0.06812278165246621	1.5155235072851215E-4
34-35	0.0	0.0	0.0	0.10358603172293807	1.5155235072851215E-4
36-37	0.0	0.0	0.0	0.15617469742573176	1.5155235072851215E-4
38-39	0.0	0.0	0.0	0.22657076433912565	1.5155235072851215E-4
40-41	0.0	0.0	0.0	0.3075754958035154	1.5155235072851215E-4
42-43	0.0	0.0	0.0	0.38085105738075103	1.5155235072851215E-4
44-45	0.0	0.0	0.0	0.45503593306235773	1.5155235072851215E-4
46-47	0.0	0.0	0.0	0.5483921811111212	1.5155235072851215E-4
48-49	0.0	7.577617536425608E-5	0.0	0.6395509200743212	1.5155235072851215E-4
50-51	0.0	1.5155235072851215E-4	0.0	0.7405605618348746	1.5155235072851215E-4
52-53	0.0	1.5155235072851215E-4	0.0	0.8345230192865523	1.5155235072851215E-4
54-55	0.0	1.5155235072851215E-4	0.0	0.9290916861411438	1.5155235072851215E-4
56-57	0.0	1.5155235072851215E-4	0.0	1.0288131329205048	1.5155235072851215E-4
58-59	0.0	1.5155235072851215E-4	0.0	1.1385370348479475	1.5155235072851215E-4
60-61	0.0	1.5155235072851215E-4	0.0	1.2481093844246618	1.5155235072851215E-4
62-63	0.0	1.5155235072851215E-4	0.0	1.3591972575086613	1.5155235072851215E-4
64-65	0.0	1.5155235072851215E-4	0.0	1.4687696070853755	1.5155235072851215E-4
66-67	0.0	1.5155235072851215E-4	0.0	1.5880413071087145	3.031047014570243E-4
68-69	0.0	1.5155235072851215E-4	0.0	1.7292123218123236	3.031047014570243E-4
70-71	0.0	1.5155235072851215E-4	0.0	1.8830379578017635	3.031047014570243E-4
72-73	0.0	1.5155235072851215E-4	0.0	2.0218599110690807	7.577617536425607E-4
74-75	0.0	1.5155235072851215E-4	0.0	2.163409806649511	8.335379290068169E-4
76-77	0.0	1.5155235072851215E-4	0.0	2.316098800008487	9.093141043710729E-4
78-79	0.0	1.5155235072851215E-4	0.0	2.485534328122964	9.093141043710729E-4
80-81	0.0	1.5155235072851215E-4	0.0	2.6639872211057867	9.85090279735329E-4
82-83	0.0	1.5155235072851215E-4	0.0	2.8557009447773547	0.0010608664550995851
84-85	0.0	1.5155235072851215E-4	0.0	3.035214704215277	0.0010608664550995851
86-87	0.0	1.5155235072851215E-4	0.0	3.2182899438953196	0.0010608664550995851
88-89	0.0	1.5155235072851215E-4	0.0	3.419854570364241	0.0012124188058280972
90-91	0.0	1.5155235072851215E-4	0.0	3.630739666402966	0.0012124188058280972
92-93	0.0	1.5155235072851215E-4	0.0	3.8565526689884484	0.0012124188058280972
94-95	0.0	1.5155235072851215E-4	0.0	4.078879967507175	0.0012124188058280972
96-97	0.0	1.5155235072851215E-4	0.0	4.30204080395491	0.0012881949811923534
98-99	0.0	1.5155235072851215E-4	0.0	4.548464926239471	0.0013639711565566093
100-101	0.0	1.5155235072851215E-4	0.0	4.810347388298339	0.0015155235072851214
102-103	0.0	1.5155235072851215E-4	0.0	5.0768521970544285	0.0015155235072851214
104-105	0.0	1.5155235072851215E-4	0.0	5.362679930528403	0.0015155235072851214
106-107	0.0	1.5155235072851215E-4	0.0	5.640399613238401	0.0015155235072851214
108-109	0.0	1.5155235072851215E-4	0.0	5.925621137309461	0.0016670758580136337
110-111	0.0	1.5155235072851215E-4	0.0	6.230771795501321	0.0016670758580136337
112-113	0.0	1.5155235072851215E-4	0.0	6.561383248615569	0.0016670758580136337
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2865	0.0	30.580393	1
AACGCAG	3385	0.0	25.35831	6
ATCAACG	3365	0.0	25.150906	3
GGTATCA	2115	0.0	25.080143	1
TCAACGC	3415	0.0	24.782665	4
CAACGCA	3415	0.0	24.608139	5
TATCAAC	3530	0.0	24.47809	2
ACGCAGA	3715	0.0	23.105755	7
ATGGGGG	1290	0.0	23.100992	5
CGCAGAG	3745	0.0	22.920664	8
GGGTATA	160	2.5799655E-4	22.350212	1
CATGGGG	2550	0.0	22.20413	4
TCTATAC	190	3.2600852E-5	21.958101	3
GTACATG	5180	0.0	21.86121	1
CATGGGT	1130	0.0	21.624983	4
ACATGGG	5235	0.0	21.289982	3
TACATGG	5300	0.0	21.25055	2
GCAGAGT	4140	0.0	20.733788	9
GTATTGA	235	7.2793628E-6	20.289553	1
ATGGGTA	605	0.0	19.702665	5
>>END_MODULE